Method for allogeneic cell therapy
A method of manipulating allogeneic cells for use in allogeneic cell therapy providing a composition of highly activated allogeneic T-cells which are infused into immunocompetent cancer patients to elicit a novel anti-tumor immune mechanism, or “Mirror Effect”. In contrast to current allogeneic cell therapy protocols where T-cells in the graft mediate the beneficial graft vs. tumor (GVT) and detrimental graft vs. host (GVH) effects, the allogeneic cells of the present invention stimulate host T-cells to mediate the “mirror” of these effects. The mirror of the GVT effect is the host vs. tumor (HVT) effect. The “mirror” of the GVH effect is the host vs. graft (HVG) effect The anti-tumor HVT effect occurs in conjunction with a non-toxic HVG rejection effect. The highly activated allogeneic cells of the invention can be used to stimulate host immunity in a complete HLA mis-matched setting in a patient.
1. A method for stimulating a coupled host vs. tumor and host vs. graft effect in a host which mirrors the coupled graft vs. tumor and graft vs. host effects of allogeneic transplant procedures comprising:
selecting a composition of donor allogeneic cells, wherein the composition of allogeneic cells include T-cells and wherein the T-cells are activated by cross-linking of CD3 and CD28 surface antigens; and
administering said allogeneic cells to the host who has not been pre-conditioned with immunosuppressive treatment.
2. The method of claim 1 wherein the T-cells are predominately CD4+ T-cells.
3. The method of claim 2 wherein the CD4+ T-cells are predominantly Th1 cells.
4. The method of claim 1 wherein the T-cells are activated at the time of infusion.