Method and device for superresolution optical measurement using singular optics
An optical method of measurement and an optical apparatus for determining the spatial position of at least one luminous object on a sample. A sequence of at least two compact luminous distributions of different topological families is projected onto the sample, and light re-emitted by the luminous object is detected. At least one optical image is generated for each luminous distribution on the basis of the light detected. The optical images are analyzed to obtain spatiotemporal information regarding the light re-emitted by the luminous object, or location of the luminous object.
1. An optical platform for determining the spatial position of at least one luminous object in a sample, the optical platform comprising:
a. a light source;
b. a biaxial crystal for generating an ordinary wave and a singular wave upon illumination by the light source;
c. coupling optics for coupling the ordinary wave and the singular wave so as to form a sequence of compact first luminous distributions and a sequence of second luminous distributions, the sequences of compact first and second luminous distributions referred to collectively as a sequence of illumination;
d. optomechanical or optical positioning means for positioning the sequence of illumination at a determined position within a volume containing a sample that includes at least one luminous object,
wherein the light source, the biaxial crystal, the coupling optics and the optomechanical or optical positioning means are each referred to as an element of the optical platform;
e. a control system associated with each element of the optical platform;
f. a detector configured to detect light re-emitted by the at least one luminous object in the sample and to generate a time-dependent detector signal;
g. a processor, characterized by mathematical parameters defining a procedure for estimation of the spatial position the at least one luminous object, configured to receive the detector signal and to obtain spatiotemporal information with respect to the light re-emitted by the at least one luminous object on the basis of the time-dependent detector signal;
h. a controller configured to control each of said control systems in such a manner as to optimize the spatiotemporal information in accordance with pre-specified criteria, as a function of measured data or of external information, by varying at least one of:
the sequence of illumination;
the determined position of the sequence of illumination; and
iii. a mathematical parameter of the processor.
2. An optical platform in accordance with claim 1 , wherein the coupling optics include a microscope.
3. An optical platform in accordance with claim 1 , wherein the coupling optics and optomechanical or optical means to position the sequence of illumination include a confocal microscope.
4. An optical platform in accordance with claim 1 , wherein the detector includes a plurality of separate detector elements.