AAV capsid proteins for nucleic acid transfer
Recombinant adeno-associated viral (AAV) capsid proteins are provided. Methods for generating the recombinant adeno-associated viral capsid proteins and a library from which the capsids are selected are also provided.
1. A method of transfer of a nucleic acid of interest into a mammal, comprising: introducing a recombinant adeno-associated virus (AAV vector into a mammal, the recombinant AAV vector encoding a nucleic acid of interest encapsidated in a capsid protein that has at least 99% sequence identity to SEQ ID NO: 31 or at least 97% sequence identity to SEQ ID NO:29.
2. The method of claim 1 , wherein the capsid protein has at least 99% sequence identity to SEQ ID NO: 31.
3. The method of claim 1 , wherein the capsid protein has at least 99% sequence identity to SEQ ID NO: 29.
4. The method of claim 1 , wherein the capsid protein is identical to SEQ ID NO: 31.
5. The method of claim 1 , wherein the capsid protein has at least 97% sequence identity to SEQ ID NO: 29.
6. The method of claim 1 , wherein the capsid protein has the sequence of SEQ ID NO: 29.
7. The method of claim 1 , where said introducing comprises introducing via injection.
8. The method of claim 7 , wherein the mammal is a human.
9. The method of claim 1 , wherein the mammal is a human.
10. A method of transfer of a nucleic acid of interest into a cell, comprising: introducing a recombinant adeno-associated virus (AAV) vector into a cell, the recombinant AAV vector encoding a nucleic acid of interest encapsidated in a capsid protein that has at least 99% sequence identity to SEQ ID NO: 31 or at least 97% sequence identity to SEQ ID NO: 29.
11. The method of claim 10 , wherein the capsid protein has at least 99% sequence identity to SEQ ID NO: 31.
12. The method of claim 10 , wherein the capsid protein has the sequence of SEQ ID NO: 31.
13. The method of claim 10 , wherein the capsid protein has at least 97% identity to SEQ ID NO: 29.
14. The method of claim 10 , where said introducing comprises introducing in vitro.
15. The method of claim 10 , where said introducing comprises introducing in vivo.