The present invention provides antigenic polypeptides expressed during an infection by a pathogenic organism, such as Acinetobacter and compositions comprising these polypeptides. The invention further provides compositions for use in treating, preventing or detecting a bacterial infection, in particular vaccine compositions using the antigenic polypeptides. The invention further provides antibodies directed to said antigenic polypeptides.
1. An immunogenic composition comprising:
a polypeptide encoded by a recombinant nucleic acid molecule consisting of the polynucleotide sequence of SEQ ID NO: 9; and,
an immuno-effective amount of an adjuvant;
wherein the composition is effective in generating an immune response against Acinetobacter baumannii in a subject in need thereof.
2. An immunogenic composition comprising:
an isolated recombinant antigenic polypeptide consisting of the amino acid sequence SEQ ID NO: 10 having immunostimulatory activity in combination with an immuno-effective amount of an adjuvant.
3. An isolated recombinant polypeptide consisting of:
a polypeptide which is encoded by a polynucleotide sequence consisting of the nucleic acid sequence of SEQ ID NO: 9 and a nucleic acid sequence encoding a His tag; or
an amino acid sequence consisting of the sequence of SEQ ID NO: 10 and a His tag.
4. The composition according to claim 1 , further comprising an effectively immunogenic delivery vehicle.
5. The composition according to claim 1 , wherein the adjuvant is selected from the group consisting of: virosomes, Freund's adjuvants (complete and incomplete), Gerbu adjuvant, mycobacteria, cholera toxin, tetanus toxoid, E. coli heat-labile toxin, quil-saponin mixtures, MF59, MALP-2, ISCOMs, aluminum hydroxide, aluminum phosphate, calcium phosphate, lysolecithin, pluronic polyols, polyanions, peptides, keyhole limpet hemocyanins, dinitrophenol, saponins, muramyl dipeptides, CpG dinucleotides, CpG oligonucleotides, monophosphoryl lipid A, polyphosphazenes, virus-like particles, and cochleates.
6. An immunogenic composition comprising:
an isolated recombinant antigenic polypeptide consisting of the amino acid sequence SEQ ID NO: 10 plus a His tag having immunostimulatory activity in combination with an immuno-effective amount of an adjuvant.