Targets of acinetobacter baumannii
View Patent ↗The present invention provides antigenic polypeptides expressed during an infection by a pathogenic organism, such as Acinetobacter and compositions comprising these polypeptides. The invention further provides compositions for use in treating, preventing or detecting a bacterial infection, in particular vaccine compositions using the antigenic polypeptides. The invention further provides antibodies directed to said antigenic polypeptides.
1. An immunogenic composition comprising:
an isolated polypeptide encoded by a recombinant nucleic acid sequence comprising a polynucleotide sequence having the nucleic acid sequence of:
SEQ ID NO: 15; and,
an immuno-effective amount of an adjuvant;
wherein the polypeptide is isolated by a process comprising (i) providing a host cell transformed/transfected with an expression vector comprising said recombinant nucleic acid sequence, (ii) growing the cell, and (iii) purifying the polypeptide from the cell or the cell's growth environment; and,
wherein the composition is effective in generating an immune response against Acinetobacter baumannii in a subject in need thereof.
2. An immunogenic composition comprising:
an isolated recombinant antigenic polypeptide consisting of the amino acid sequence SEQ ID NO: 16 having immunostimulatory activity in combination with an immuno-effective amount of an adjuvant; and,
one or more polypeptides selected from the group consisting of: SEQ ID NOs: 2, 4, 6, 8, 10, 12, and 14.
3. The composition according to claim 1 , further comprising an effectively immunogenic delivery vehicle.
4. The composition according to claim 1 , wherein the adjuvant is selected from the group consisting of: virosomes, Freund's adjuvants (complete and incomplete), Gerbu adjuvant, mycobacteria, cholera toxin, tetanus toxoid, E. coli heat-labile toxin, quil-saponin mixtures, MF59, MALP-2, ISCOMs, aluminum hydroxide, aluminum phosphate, calcium phosphate, lysolecithin, pluronic polyols, polyanions, peptides, keyhole limpet hemocyanins, dinitrophenol, saponins, muramyl dipeptides, CpG dinucleotides, CpG oligonucleotides, monophosphoryl lipid A, polyphosphazenes, virus-like particles, and cochleates.
5. The composition according to claim 1 , wherein the recombinant nucleic acid sequence further comprises a sequence encoding a His tag.
6. The composition of claim 1 , further comprising one or more polypeptides selected from the group consisting of: SEQ ID NOs: 2, 4, 6, 8, 10, 12, and 14.
7. The composition of claim 1 , wherein the composition does not comprise any additional Acinetobacter baumannii polypeptides.