Methods of propagating rhinovirus C in previously unsusceptible cell lines
The present invention provides methods of propagating rhinovirus C (RV-C) in a host cell; a host cell comprising an effective amount of a heterologous CDHR3 receptor such that the host cell can support propagation of rhinovirus C; and kits comprising at least one host cell previously unable to support rhinovirus C growth, wherein the host cell comprises a heterologous CDHR3 receptor and a sample of rhinovirus C. Methods of use are also provided.
1. A kit comprising:
a. at least one host cell able to support rhinovirus C replication, wherein the host cell comprises a heterologous CDHR3 receptor; and
b. a sample of rhinovirus C or rhinovirus C variant.
2. The kit of claim 1 wherein the host cell is selected from the group consisting of NCI-H358, WI-38, WisL, HEK293T, BEAS-2B, A549 and HeLa.
3. The kit of claim 1 wherein the CDHR3 receptor is a CDHR3-C529Y variant.
4. A kit comprising:
a. a nucleic acid encoding a CDHR3 receptor; and
b. a sample of rhinovirus C.
5. The kit of claim 4 wherein the CDHR3 receptor is a CDHR3-C529Y variant.
6. A host cell infected with rhinovirus C or rhinovirus C variant, wherein the host cell has been modified to express an effective amount of a CDHR3 receptor and support propagation of rhinovirus C.
7. The host cell of claim 6 wherein the CDHR3 receptor is a CDHR3-C529Y variant.
8. The transfected cell of claim 6 wherein the host cell is a cell able to support rhinovirus C replication.