IP Library › Granted Patent US 10,202,425
Granted Patent B2
US 10,202,425 · App. 15/926,892 · Granted Feb 12, 2019

Selective recovery

Inventors: Benjamin E. Deverman (Pasadena, CA); Paul H. Patterson (Altadena, CA); Viviana Gradinaru (La Cañada Flintridge, CA)
Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
C07K14/005A61K38/1709A61K38/2093A61K38/47A61K38/4813A61K38/50A61K39/3955A61K48/005A61K48/0058C07K7/06C12N7/00C12N15/1068C12N15/86A61K38/00C07K2319/33C12N2750/14122C12N2750/14143C12N2750/14145C12N2810/6027C12Y304/14009C12Y305/01015
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Quick Facts
Patent No.
US 10,202,425
App. No.
15/926,892
Granted
Feb 12, 2019
Kind
B2
Abstract

Provided herein are methods of selective screening. In addition, various targeting proteins and sequences, as well as methods of their use, are also provided.

Claims (34)

1. An AAV vector comprising a capsid protein, said capsid protein comprises an amino acid sequence selected from the group consisting of ILGTGTS (SEQ ID NO: 55), NGGTSSS (SEQ ID NO: 58), YTLSQGW (SEQ ID NO: 60), and FTLTTPK (SEQ ID NO: 29).

2. The AAV vector of claim 1 , wherein the capsid protein is part of an AAV9 capsid protein.

3. The AAV vector of claim 2 , wherein the amino acid sequence is inserted into the unstructured surface loop of the AAV9 capsid protein.

4. The AAV vector of claim 2 , wherein the amino acid sequence is inserted within amino acids 452-458, or between amino acids 586-592, 262-269, 464-473, 491-495, 546-557, or 659-668 of the AAV9 capsid protein, and wherein the AAV9 capsid protein comprises SEQ ID NO: 2.

5. The AAV vector of claim 4 , wherein the amino acid sequence is inserted between AA586-592 of the AAV9 capsid protein.

6. The AAV vector of claim 4 , wherein the amino acid sequence is inserted between AA588-589 of the AAV9 capsid protein.

7. The AAV vector of claim 4 , wherein the amino acid sequence is inserted within amino acids 452-458 of the AAV9 capsid protein.

8. The AAV vector of claim 1 , wherein the amino acid sequence is ILGTGTS (SEQ ID NO: 55).

9. The AAV vector of claim 8 , wherein the capsid protein is part of an AAV9 capsid protein, wherein the amino acid sequence is inserted within amino acids 452-458 of an AAV9 capsid protein, and wherein the AAV9 capsid protein comprises SEQ ID NO: 2.

10. The AAV vector of claim 1 , wherein the amino acid sequence is NGGTSSS (SEQ ID NO: 58).

11. The AAV vector of claim 10 , wherein the capsid protein is part of an AAV9 capsid protein, wherein the amino acid sequence is inserted within amino acids 588-589 of an AAV9 capsid protein, and wherein the AAV9 capsid protein comprises SEQ ID NO: 2.

12. The AAV vector of claim 1 , wherein the amino acid sequence is YTLSQGW (SEQ ID NO: 60).

13. The AAV vector of claim 12 , wherein the capsid protein is part of an AAV9 capsid protein, wherein the amino acid sequence is inserted within amino acids 588-589 of an AAV9 capsid protein, and wherein the AAV9 capsid protein comprises SEQ ID NO: 2.

14. The AAV vector of claim 1 , wherein the amino acid sequence is FTLTTPK (SEQ ID NO: 29).

15. The AAV vector of claim 14 , wherein the capsid protein is part of an AAV9 capsid protein, wherein the amino acid sequence is inserted within amino acids 588-589 of an AAV9 capsid protein, and wherein the AAV9 capsid protein comprises SEQ ID NO: 2.

16. The AAV vector of claim 1 , wherein the vector comprises an rAAV genome.

17. The AAV vector of claim 16 , wherein the rAAV genome comprises at least one inverted terminal repeat.

18. An AAV capsid protein comprising an amino acids sequences selected from the group consisting of: ILGTGTS (SEQ ID NO: 55), NGGTSSS (SEQ ID NO: 58), YTLSQGW (SEQ ID NO: 60), and FTLTTPK (SEQ ID NO: 29).

19. The AAV capsid protein of claim 18 , further conjugated to a nanoparticle or a second molecule.

20. The AAV capsid protein of claim 18 , wherein the AAV is selected from the group consisting of AAV1, AAV2, AAV3, AAV3b, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, human isolate hu.31, human isolate hu.32, rhesus isolate rh.8, and rhesus isolate rh.10.

21. The AAV capsid protein of claim 20 , wherein the AAV is an AAV9.

22. The AAV capsid protein of claim 21 , wherein the amino acid sequence is inserted into the unstructured surface loop of AAV9.

23. The AAV capsid protein of claim 22 , wherein the amino acid sequence is inserted within amino acids 452-458, or between amino acids 586-592, 262-269, 464-473, 491-495, 546-557, or 659-668 of the AAV9 capsid protein, wherein the AAV9 capsid protein comprises SEQ ID NO: 2.

24. The AAV capsid protein of claim 22 , wherein the amino acid sequence is inserted between amino acids 586-592 of the AAV9 capsid protein.

25. The AAV capsid protein of claim 22 , wherein the amino acid sequence is inserted between amino acids 588-589 of the AAV9 capsid protein.

26. The AAV capsid protein of claim 22 , wherein the amino acid sequence is inserted within amino acids 452-458 of the AAV9 capsid protein.

27. The AAV capsid protein of claim 18 , wherein the amino acid sequence is ILGTGTS (SEQ ID NO: 55).

28. The AAV capsid protein of claim 27 , wherein the amino acid sequence is inserted within amino acids 452-458 of an AAV9 capsid protein wherein the AAV9 capsid protein comprises SEQ ID NO: 2.

29. The AAV capsid protein of claim 18 , wherein the amino acid sequence is NGGTSSS (SEQ ID NO: 58).

30. The AAV capsid protein of claim 29 , wherein the amino acid sequence is inserted between amino acids 588-589 of an AAV9 capsid protein wherein the AAV9 capsid protein comprises SEQ ID NO: 2.

31. The AAV capsid protein of claim 18 , wherein the amino acid sequence is YTLSQGW (SEQ ID NO: 60).

32. The AAV capsid protein of claim 31 , wherein the amino acid sequence is inserted between amino acids 588-589 of an AAV9 capsid protein wherein the AAV9 capsid protein comprises SEQ ID NO: 2.

33. The AAV capsid protein of claim 18 , wherein the amino acid sequence is FTLTTPK (SEQ ID NO: 29).

34. The AAV capsid protein of claim 33 , wherein the amino acid sequence is inserted between amino acids 588-589 of an AAV9 capsid protein wherein the AAV9 capsid protein comprises SEQ ID NO: 2.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 21, 2018
From: DEVERMAN, BENJAMIN E.; PATTERSON, PAUL H.; GRADINARU, VIVIANA
To: CALIFORNIA INSTITUTE OF TECHNOLOGY
Reel/Frame 045305/0974 →
Continuity (7)
Continuation 15422237 · Feb 1, 2017
Continuation 14485024 · Sep 12, 2014
Provisional Application 61877506 · Sep 13, 2013
Provisional Application 61983624 · Apr 24, 2014
Provisional Application 62020658 · Jul 3, 2014
Provisional Application 62034060 · Aug 6, 2014
Related Publication 20180230186A1 · Aug 16, 2018
Cited By (2)
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