Stabilized hypochlorous acid solution and use thereof
Disclosed is an antimicrobial solution comprising, consisting essentially of, or consisting of (a) hypochlorous acid, (b) a divalent cation, (c) phosphate anion, and (d) water, as well as a process for its production. Also disclosed is a method of treating or preventing infection associated with abdominal surgery on a mammal, comprising use of the antimicrobial solution.
1. An antimicrobial solution consisting of:
(a) about 10 mg/L to about 500 mg/L of hypochlorous acid,
(b) about 20 mg/L to about 250 mg/L of a divalent cation selected from calcium, magnesium, and a combination thereof,
(c) about 200 mg/L to about 1000 mg/L of a phosphate anion calculated on the basis of phosphate anion formulated as PO 4 3− ,
(d) optionally, chloride ion, and
(e) water,
wherein the solution has a pH of about 3 to about 7.
2. The solution of claim 1 , wherein the solution comprises about 50 mg/L to about 250 mg/L of hypochlorous acid.
3. The solution of claim 1 , wherein the magnesium is provided by magnesium sulfate and the calcium is provided by calcium sulfate.
4. The solution of claim 1 , wherein the phosphate anion is provided by sodium dihydrogen phosphate.
5. The solution of claim 1 , wherein the solution further comprises about 10 mg/L to about 400 mg/L of chloride ion.
6. The solution of claim 1 , consisting of:
(a) about 10 mg/L to about 500 mg/L of hypochlorous acid,
(b) magnesium sulfate in an amount sufficient to provide about 20 mg/L to about 250 mg/L of magnesium,
(c) sodium dihydrogen phosphate is present in an amount sufficient to provide about 200 mg/L to about 1000 mg/L of phosphate anion calculated on the basis of phosphate anion formulated as PO 4 3− ,
(d) about 20 mg/L to about 100 mg/L of chloride ion, and
(e) water,
wherein the solution has a pH of about 4.5 to about 5.5.
7. A pharmaceutical composition comprising the solution of claim 1 and a pharmaceutically acceptable carrier.
8. A sealed container comprising the solution of claim 1 .
9. A process for the production of an antimicrobial solution comprising the steps of:
(i) providing a mixture comprising (a) about 20 mg/L to about 250 mg/L of a divalent cation selected from magnesium, calcium, and a combination thereof, (b) about 200 mg/L to about 1000 mg/L of a phosphate anion calculated on the basis of phosphate anion formulated as PO 4 3− , (c) a pH-adjusting agent, and (d) water, and
(ii) adding chlorine gas to the mixture,
wherein the process produces a solution comprising hypochlorous acid in an amount from about 10 mg/L to about 500 mg/L and having a pH from about 3 to about 7.
10. The process of claim 9 , wherein the process produces a solution comprising hypochlorous acid in an amount from about 50 mg/L to about 250 mg/L.
11. The process of claim 9 , wherein the magnesium is provided by magnesium sulfate and the calcium is provided by calcium sulfate.
12. The process of claim 9 , wherein the phosphate anion is provided by sodium dihydrogen phosphate.
13. The process of claim 9 , wherein the solution further comprises about 20 mg/L to about 100 mg/L of chloride ion.
14. The process of claim 9 , wherein the chlorine gas is bubbled into the buffer solution.
15. A process for the production of an antimicrobial solution comprising the steps of:
(i) providing a mixture comprising hypochlorous acid and water, and
(ii) adding (a) about 20 mg/L to about 250 mg/L of a divalent cation selected from magnesium, calcium, and a combination thereof, (b) about 200 mg/L to about 1000 mg/L of a phosphate anion calculated on the basis of phosphate anion formulated as PO 4 3− , and (c) a pH-adjusting agent to the mixture,
wherein the process produces a solution comprising hypochlorous acid in an amount from about 10 mg/L to about 500 mg/L and having a pH from about 3 to about 7.
16. The process of claim 15 , wherein the hypochlorous acid is electrochemically generated.
17. The process of claim 15 , wherein the process produces a solution comprising hypochlorous acid in an amount from about 50 mg/L to about 250 mg/L.
18. The process of claim 15 , wherein the magnesium is provided by magnesium sulfate and the calcium is provided by calcium sulfate.
19. The process of claim 15 , wherein the phosphate anion is provided by sodium dihydrogen phosphate.
20. The process of claim 15 , wherein the solution further comprises about 20 mg/L to about 100 mg/L of chloride ion.
21. A method of treating or preventing infection associated with abdominal surgery on a mammal, comprising administering to the mammal an effective amount of the solution of claim 1 .
22. The method of claim 21 , wherein the surgery is inguinal hernia surgery, exploratory laparotomy, appendectomy, or laparoscopy.
23. The method of claim 21 , wherein the infection is caused by an organism selected from methicillin-resistant S. aureus, E. coli, P. aeruginosa , Vancomycin-resistant Enterococcus, P. mirabilis, S. marcescens , and C. albicans.
24. A sealed container comprising the composition of claim 7 .