Methods and kits for extraction of DNA
Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.
1. A kit for isolating and purifying nucleic acid from a sample comprising biological material, comprising a starting solution comprising a soluble polymer and detergent; a polar solvent comprising ethanol; and dextran-encased magnetically attractable particles.
2. The kit of claim 1 , wherein the polymer comprises one or more of dextran, cellulose, cellulose derivatives, soluble starch, dextrin, cellodextrin, polyethylene glycol, heparin, glycogen, and combinations thereof.
3. The kit of claim 1 , wherein the detergent comprises one or more of N-lauroyl sarcosine, sarcosyl, deoxycholate, CTAB, deoxycholate, dodecyl β-D-maltoside, nonanoyl-N-methylglucamide, polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether, sodium dodecyl sulfate, and combinations thereof.
4. The kit of claim 1 , wherein all of the contents of the kit are in one container.
5. The kit of claim 1 , further comprising a lysis solution.
6. The kit of claim 1 , further comprising a wash solution.