Methods and compositions relating to synthetic beta-1,6 glucosamine oligosaccharides
The invention relates to the compositions of synthetic oligo-β-(1→6)-2-amino-2-deoxy-D-glu-copyranosides conjugated to carriers, and methods for making and use same.
1. An oligosaccharide-carrier conjugate comprising an oligosaccharide conjugated to a carrier that is tetanus toxoid, through a linker, wherein the oligosaccharide is a β-1,6-linked glucosamine that is 0-40% acetylated and 5-11 monomers in length.
2. The oligosaccharide-carrier conjugate of claim 1 , wherein the oligosaccharide is 5 monomers in length.
3. The oligosaccharide-carrier conjugate of claim 1 , wherein the oligosaccharide is 0% acetylated.
4. The oligosaccharide-carrier conjugate of claim 1 , wherein the oligosaccharide-carrier conjugate has an oligosaccharide to carrier ratio of 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, 20:1, 30:1, 40:1, 50:1, 60:1, 70:1, 80:1, 90:1 or 100:1.
5. The oligosaccharide-carrier conjugate of claim 1 , wherein the oligosaccharide-carrier conjugate has an oligosaccharide to carrier ratio in the range of 10:1 to 50:1.
6. The oligosaccharide-carrier conjugate of claim 1 , free of microbial contaminants other than tetanus toxoid or components thereof.