IP Library › Granted Patent US 12,397,014
Granted Patent B2
US 12,397,014 · App. 17/427,763 · Granted Aug 26, 2025

Polysaccharide compositions for use in treating filariasis

Inventors: Gerald B. Pier (Brookline, MA); Colette Cywes Bentley (Brookline, MA)
Assignee: The Brigham and Women's Hospital, Inc.
A61K31/715A61K47/6415A61P31/00C07K16/18C07K2317/73
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Quick Facts
Patent No.
US 12,397,014
App. No.
17/427,763
Granted
Aug 26, 2025
Kind
B2
Abstract

The disclosure relates, in part, to the use of compositions of poly N-acetylated glucosamine (PNAG), deacetylated PNAG (dPNAG), and antibodies and antibody fragments specific to PNAG in the prevention and treatment of filariasis.

Claims (18)

1. A method for treating a subject having filariasis, comprising

administering to a subject having filariasis a deacetylated poly-N-acetyl glucosamine (dPNAG) conjugated to a carrier compound in an effective amount to treat filariasis in the subject,

wherein the dPNAG has a structure

wherein n is at least 4, R is selected from the group consisting of —NH—CO—CH 3 and —NH 2 , provided that less than 50% of the R groups are —NH—CO—CH 3 .

2. The method of claim 1 , wherein the dPNAG is conjugated to the carrier compound through a linker.

3. The method of claim 1 , wherein the carrier compound is a peptide or protein carrier.

4. The method of claim 3 , wherein the peptide or protein carrier is tetanus toxoid.

5. The method of claim 1 , wherein less than 30%, less than 20%, less than 10%, or less than 5% of R groups are —NH—CO—CH 3 .

6. The method of claim 1 , wherein the R group is —NH 2 .

7. The method of claim 1 , wherein n is 4.

8. The method of claim 1 , wherein n is 5, 6, 7, or 8.

9. The method of claim 1 , wherein a plurality of the dPNAG are conjugated to the carrier compound, and wherein the carrier compound is a peptide or protein carrier.

10. The method of claim 1 , wherein the subject is a dog or a cat.

11. The method of claim 10 , wherein the filariasis is caused by Dirofilaria immitis, Acanthocheilonema spp., or Brugia spp.

12. The method of claim 10 , wherein the filariasis is caused by Dirofilaria immitis that is resistant to an anti-filariasis medicament.

13. The method of claim 10 , wherein the filariasis is caused by Dirofilaria immitis that is resistant to ivermectin, avermectin, and/or milbemycin.

14. The method of claim 1 , wherein the dPNAG is administered with an adjuvant.

15. A method for treating a subject having filariasis, comprising administering to a subject having filariasis an effective amount of an antibody or antibody fragment that binds to poly-N-acetyl glucosamine (PNAG) and deacetylated PNAG (dPNAG).

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 21, 2025
From: PIER, GERALD B.; BENTLEY, COLETTE CYWES
To: THE BRIGHAM AND WOMEN'S HOSPITAL, INC.
Reel/Frame 071775/0716 →
Continuity (2)
Provisional Application 62801399 · Feb 5, 2019
Related Publication 20220117997A1 · Apr 21, 2022
References Cited (400)
US 2842049A · Delangre · 1958 [cited by applicant]
US 4197290A · Yoshida · 1980 [cited by applicant]
US 4285936A · Pier et al. · 1981 [cited by applicant]
US 4379145A · Masuho et al. · 1983 [cited by applicant]
US 4443549A · Sadowski · 1984 [cited by applicant]
US 4465776A · Cidlowski et al. · 1984 [cited by applicant]
US 4578458A · Pier · 1986 [cited by applicant]
US 4652448A · Sadowski · 1987 [cited by applicant]
US 4755381A · Cryz · 1988 [cited by applicant]
US 4786592A · Deal et al. · 1988 [cited by applicant]
US 4789735A · Frank et al. · 1988 [cited by applicant]
US 4795803A · Lindberg et al. · 1989 [cited by applicant]
US 4830852A · Marburg et al. · 1989 [cited by applicant]
US 4859449A · Mattes · 1989 [cited by applicant]
US 4879272A · Shimoda et al. · 1989 [cited by applicant]
US 4902616A · Fournier et al. · 1990 [cited by applicant]
US 5055455A · Pier · 1991 [cited by applicant]
US 5352670A · Venot et al. · 1994 [cited by applicant]
US 5362754A · Raad et al. · 1994 [cited by applicant]
US 5366505A · Farber · 1994 [cited by applicant]
US 5425946A · Tai et al. · 1995 [cited by applicant]
US 5571511A · Fischer · 1996 [cited by applicant]
US 5589591A · Lewis · 1996 [cited by applicant]
US 5688516A · Raad et al. · 1997 [cited by applicant]
US 5718694A · Rupp · 1998 [cited by applicant]
US 5763191A · Knoll et al. · 1998 [cited by applicant]
US 5830539A · Yan et al. · 1998 [cited by applicant]
US 5844093A · Kettleborough et al. · 1998 [cited by applicant]
US 5858350A · Vournakis et al. · 1999 [cited by applicant]
US 5866140A · Fattom et al. · 1999 [cited by applicant]
US 5872215A · Osbourne et al. · 1999 [cited by applicant]
US 5980910A · Pier · 1999 [cited by applicant]
US 5989542A · Pier et al. · 1999 [cited by applicant]
US 6235883B1 · Jakobovits et al. · 2001 [cited by applicant]
US 6245735B1 · Pier · 2001 [cited by applicant]
US 6399066B1 · Pier · 2002 [cited by applicant]
US 6743431B2 · Pier · 2004 [cited by applicant]
US 6822072B1 · Edwards et al. · 2004 [cited by applicant]
US 6903194B1 · Sato et al. · 2005 [cited by applicant]
US 6924360B2 · Green et al. · 2005 [cited by applicant]
US 7015007B2 · Pier et al. · 2006 [cited by applicant]
US 7157443B2 · Joyce et al. · 2007 [cited by applicant]
US 7252828B2 · Pier et al. · 2007 [cited by applicant]
US 7550569B2 · Baker et al. · 2009 [cited by applicant]
US 7723087B2 · Pier et al. · 2010 [cited by applicant]
US 7786255B2 · Pier et al. · 2010 [cited by applicant]
US 8084595B2 · Pier et al. · 2011 [cited by applicant]
US 8252894B2 · Pier et al. · 2012 [cited by applicant]
US 8350017B2 · Pier et al. · 2013 [cited by applicant]
US 8410249B2 · Pier et al. · 2013 [cited by applicant]
US 8435515B2 · Pier et al. · 2013 [cited by applicant]
US 8454979B2 · Mitarai et al. · 2013 [cited by applicant]
US 8461319B2 · Pier et al. · 2013 [cited by applicant]
US 8492364B2 · Pier et al. · 2013 [cited by applicant]
US 8658775B2 · Baker et al. · 2014 [cited by applicant]
US 8663654B2 · Pier et al. · 2014 [cited by applicant]
US 8703148B2 · Biemans et al. · 2014 [cited by applicant]
US 8912314B2 · Pier et al. · 2014 [cited by applicant]
US 8933218B2 · Biemans et al. · 2015 [cited by applicant]
US 9458227B2 · Pier et al. · 2016 [cited by applicant]
US 9474806B2 · Pier · 2016 [cited by examiner]
US 10017563B2 · Pier et al. · 2018 [cited by applicant]
US 10034927B2 · Pier · 2018 [cited by examiner]
US 10906962B2 · Pier et al. · 2021 [cited by applicant]
US 10919956B2 · Pier et al. · 2021 [cited by applicant]
US 11123416B2 · Pier et al. · 2021 [cited by applicant]
US 20010048929A1 · Chong et al. · 2001 [cited by applicant]
US 20020031528A1 · Fattom · 2002 [cited by applicant]
US 20020119166A1 · Pier et al. · 2002 [cited by applicant]
US 20030003100A1 · Levy et al. · 2003 [cited by applicant]
US 20030031528A1 · Kram · 2003 [cited by applicant]
US 20030113350A1 · Fattom et al. · 2003 [cited by applicant]
US 20030124631A1 · Pier et al. · 2003 [cited by applicant]
US 20040005632A1 · Erlanson et al. · 2004 [cited by applicant]
US 20040018198A1 · Gudas et al. · 2004 [cited by applicant]
US 20040091494A1 · Pier et al. · 2004 [cited by applicant]
US 20040175731A1 · Pier et al. · 2004 [cited by applicant]
US 20050025775A1 · Pier et al. · 2005 [cited by applicant]
US 20050118198A1 · Pier et al. · 2005 [cited by applicant]
US 20060115486A1 · Pier et al. · 2006 [cited by applicant]
US 20090162341A1 · Foster et al. · 2009 [cited by applicant]
US 20100021503A1 · Denoel et al. · 2010 [cited by applicant]
US 20100303852A1 · Biemans et al. · 2010 [cited by applicant]
US 20100322959A1 · Biemans et al. · 2010 [cited by applicant]
US 20110008385A1 · Castado et al. · 2011 [cited by applicant]
US 20110150880A1 · Pier et al. · 2011 [cited by applicant]
US 20120189700A1 · Aguilar et al. · 2012 [cited by applicant]
US 20130243806A1 · Pier et al. · 2013 [cited by applicant]
US 20140037633A1 · Pier et al. · 2014 [cited by applicant]
US 20140206016A1 · Sanchez et al. · 2014 [cited by applicant]
US 20150165016A1 · Pier et al. · 2015 [cited by applicant]
US 20150329620A1 · Pier et al. · 2015 [cited by applicant]
US 20160375117A1 · Pier et al. · 2016 [cited by applicant]
US 20170226194A1 · Pier et al. · 2017 [cited by applicant]
US 20180043023A1 · Ilyinskii · 2018 [cited by examiner]
US 20190117754A1 · Pier et al. · 2019 [cited by applicant]
US 20190127448A1 · Pier et al. · 2019 [cited by applicant]
US 20210363228A1 · Pier et al. · 2021 [cited by applicant]
US 20220175904A1 · Pier et al. · 2022 [cited by applicant]
US 20230382980A1 · Pier et al. · 2023 [cited by applicant]
US 20240358811A1 · Pier et al. · 2024 [cited by applicant]
CA 2480052A1 · 2003 [cited by applicant]
CA 2475736A1 · 2006 [cited by applicant]
CN 1252807 · 2000 [cited by applicant]
CN 1344722A · 2002 [cited by applicant]
CN 101001874A · 2007 [cited by applicant]
CN 100351260C · 2007 [cited by applicant]
DE 69113102T2 · 1996 [cited by applicant]
EP 0302781A1 · 1989 [cited by applicant]
EP 0574000A1 · 1993 [cited by applicant]
EP 0694309A2 · 1996 [cited by applicant]
EP 2315747A2 · 2011 [cited by applicant]
FR 2410043A1 · 1979 [cited by applicant]
FR 2581877A1 · 1986 [cited by applicant]
FR 2640628A1 · 1990 [cited by applicant]
GB 2009771A · 1979 [cited by applicant]
JP H0222234 · 1990 [cited by applicant]
JP H11509861A · 1999 [cited by applicant]
JP H11322793A · 1999 [cited by applicant]
JP 2001500528A · 2001 [cited by applicant]
JP 2002503705A · 2002 [cited by applicant]
JP 2002520374A · 2002 [cited by applicant]
JP 2005515237A · 2005 [cited by applicant]
JP 2006513166A · 2006 [cited by applicant]
JP 2008501317A · 2008 [cited by applicant]
KR 1020050074582A · 2005 [cited by applicant]
WO WO8505037A1 · 1985 [cited by applicant]
WO WO8602358A1 · 1986 [cited by applicant]
WO WO8802028A1 · 1988 [cited by applicant]
WO WO8904873A1 · 1989 [cited by applicant]
WO WO9003398A1 · 1990 [cited by applicant]
WO WO9006696A2 · 1990 [cited by applicant]
WO WO9301276A1 · 1993 [cited by applicant]
WO WO9309811A1 · 1993 [cited by applicant]
WO WO9319373A1 · 1993 [cited by applicant]
WO WO9415640A1 · 1994 [cited by applicant]
WO WO9717334A1 · 1997 [cited by applicant]
WO WO9719105A1 · 1997 [cited by applicant]
WO WO9847915A1 · 1998 [cited by applicant]
WO WO9852605A1 · 1998 [cited by applicant]
WO WO9940440A1 · 1999 [cited by applicant]
WO WO9942130A1 · 1999 [cited by applicant]
WO WO0003745A2 · 2000 [cited by applicant]
WO WO0035504A1 · 2000 [cited by applicant]
WO WO0056360A2 · 2000 [cited by applicant]
WO WO0141800A2 · 2001 [cited by applicant]
WO WO2002094983A2 · 2002 [cited by applicant]
WO WO2003053462A2 · 2003 [cited by applicant]
WO WO2003061558A2 · 2003 [cited by applicant]
WO WO2003080672A1 · 2003 [cited by applicant]
WO WO2003085093A2 · 2003 [cited by applicant]
WO WO2003087054A2 · 2003 [cited by applicant]
WO WO2004043405A2 · 2004 [cited by applicant]
WO WO2004043407A2 · 2004 [cited by applicant]
WO WO2004080490A2 · 2004 [cited by applicant]
WO WO2005000346A1 · 2005 [cited by applicant]
WO WO2005016973A1 · 2005 [cited by applicant]
WO WO2005103084A2 · 2005 [cited by applicant]
WO WO2006032472A2 · 2006 [cited by applicant]
WO WO2006065503A2 · 2006 [cited by applicant]
WO WO2006065553A2 · 2006 [cited by applicant]
WO WO2006096970A1 · 2006 [cited by applicant]
WO WO2006100108A1 · 2006 [cited by applicant]
WO WO2007113223A2 · 2007 [cited by applicant]
WO WO2007113224A2 · 2007 [cited by applicant]
WO WO2010011284A2 · 2010 [cited by examiner]
WO WO2013181348A1 · 2013 [cited by applicant]
Butt et al., [cited by applicant]
Pier, Excerpt from Chapter 2: Molecular Mechanisms of Microbial Pathogenesis. Harrison's Infectious Diseases. Eds. Dennis L. Kasper and Anthony S. Fauci. McGraw-Hill Companies, Inc. New York. 2010:9-12. [cited by applicant]
Sevrin et al., Disseminated [cited by applicant]
Tang et al., The Relationship Between Staphylococcal Biofilm Formation Mechanism and ica locus. Microbiology China. 2008;35(8):1287-91. [cited by applicant]
U.S. Appl. No. 10/713,790, filed Nov. 12, 2003, Granted, U.S. Pat. No. 10,919,956. [cited by applicant]
U.S. Appl. No. 10/712,391, filed Nov. 12, 2003, Granted, U.S. Pat. No. 7,723,087. [cited by applicant]
U.S. Appl. No. 11/111,688, filed Apr. 21, 2005, Granted, U.S. Pat. No. 7,786,255. [cited by applicant]
U.S. Appl. No. 12/824,510, filed Jun. 28, 2010, Granted, U.S. Pat. No. 8,084,595. [cited by applicant]
U.S. Appl. No. 13/103,532, filed May 9, 2011, Granted, U.S. Pat. No. 8,252,894. [cited by applicant]
U.S. Appl. No. 14/548,173, filed Nov. 19, 2014, Granted, U.S. Pat. No. 9,458,227. [cited by applicant]
U.S. Appl. No. 16/005,647, filed Jun. 11, 2018, Granted, U.S. Pat. No. 10,906,962. [cited by applicant]
U.S. Appl. No. 17/131,613, filed Dec. 22, 2020, Published, 2021-0363228. [cited by applicant]
U.S. Appl. No. 13/055,178, filed Mar. 2, 2011, Granted, U.S. Pat. No. 8,492,364. [cited by applicant]
U.S. Appl. No. 13/924,435, filed Jun. 21, 2013, Granted, U.S. Pat. No. 9,474,806. [cited by applicant]
U.S. Appl. No. 15/258,417, filed Sep. 7, 2016, Granted, U.S. Pat. No. 10,034,927. [cited by applicant]
U.S. Appl. No. 16/016,473, filed Jun. 22, 2018, Granted, U.S. Pat. No. 11,123,416. [cited by applicant]
U.S. Appl. No. 17/408,287, filed Aug. 20, 2021, Pending. [cited by applicant]
U.S. Appl. No. 14/404,303, filed Nov. 26, 2014, Published, 2015-0165016. [cited by applicant]
U.S. Appl. No. 12/294,689, filed Sep. 26, 2008, Granted, U.S. Pat. No. 8,703,148. [cited by applicant]
U.S. Appl. No. 12/294,643, filed Sep. 26, 2008, Granted, U.S. Pat. No. 8,933,218. [cited by applicant]
PCT/US2020/016863, May 7, 2020, International Search Report and Written Opinion. [cited by applicant]
PCT/US2020/016863, Aug. 19, 2021, International Preliminary Report on Patentability. [cited by applicant]
International Search Report and Written Opinion mailed May 7, 2020 for Application No. PCT/US2020/016863. [cited by applicant]
International Preliminary Report on Patentability mailed Aug. 19, 2021 for Application No. PCT/US2020/016863. [cited by applicant]
[No Author Listed] ATCC Catalogue website 2001; ATCC No. 35984. [cited by applicant]
[No Author Listed] ATCC Catalogue: Bacteria and Bacteriophages; 1992; 18 [cited by applicant]
[No. Author Listed] Five Surprising Facts about Pneumococcal Pneumonia. American Lung Association. Nov. 2017. Available at http://www.lung.org/about-us/media/top-stories/five-surprising-facts-about-pneumococcal-pneumoni… [cited by applicant]
[No Author Listed] Pneumococcal Disease, Transmission and Those at High Risk. CDC Control and Prevention Sep. 2017. Available at http://www.cdc.gov/pneumococcal/about/risk-transmission.html. Last accessed Mar. 8, 2018. … [cited by applicant]
No Author Listed, The New Riverside University Dictionary, The Riverside Publishing Company, p. 933, 1984. [cited by applicant]
No Author Listed, Illustrated Stedman's Medical Dictionary, 24 [cited by applicant]
No Author Listed, New York Times Editorial, Another Very Scary Germ. Nov. 20, 2007. Available at http://www.nytimes.com/2007/11/20/opinion/20tue2.html_r=2&oref=slogin&pagewanted. Last accessed Apr. 30, 2010. 2 pages. [cited by applicant]
[No Author Listed] Adis R&D Profile Drug in R&D. Adis International. 2003;4(6):383-5. Available at http://www.ingentaconnect.com/content/adis/rdd//2003/00000004/00000006/art00013. Last accessed Nov. 11, 2010. Abstract o… [cited by applicant]
GenBank Submission; NIH/BCBI; Accession No. AAZ87998; Pier et al; May 31, 2000. [cited by applicant]
GenBank Submission; NIH/NCBI, Accession No. BA000018; Kuroda et al.; Oct. 22, 2004 (last submission). [cited by applicant]
GenBank Submission; NIH/NCBI Accession No. AF086783. Oct. 1, 1999. Cramton et al. [cited by applicant]
GenBank Submission; NIH/NCBI, Accession No. DQ231549; Kelly-Quintos et al.; Printed May 9, 2006. [cited by applicant]
GenBank Submission; NIH/NCBI, Accession No. DQ231550; Kelly-Quintos et al.; Printed May 9, 2006. [cited by applicant]
GenBank Submission; NIH/NCBI, Accession No. DQ231551; Kelly-Quintos et al.; Printed May 9, 2006. [cited by applicant]
GenBank Submission; NIH/NCBI, Accession No. DQ231552; Kelly-Quintos et al.; Printed May 9, 2006. [cited by applicant]
GenBank Submission; NIH/NCBI, Accession No. DQ231553; Kelly-Quintos et al.; Printed May 9, 2006. [cited by applicant]
GenBank Submission; NIH/NCBI, Accession No. DQ231554; Kelly-Quintos et al.; Printed May 9, 2006. [cited by applicant]
EBI DBfetch submission; EMBL-EBI; Accession No. U43366; Heilmann et al, Apr. 17, 2005 (last submission). [cited by applicant]
Allignet et al., Tracking adhesion factors in [cited by applicant]
Ammendolia et al., Slime production and expression of the slime-associated antigen by staphylococcal clinical isolates. J Clin Microbiol. Oct. 1999;37(10):3235-8. [cited by applicant]
Arciola et al., In catheter infections by [cited by applicant]
Baldassarri et al., Purification and characterization of the staphylococcal slime-associated antigen and its occurrence among [cited by applicant]
Barsham et al., Detection of antibodies to [cited by applicant]
Bernstein, et al., Antibody coated bacteria in otitis media with effusions. Ann Otol Rhinol Laryngol Suppl. May-Jun. 1980;89(3 Pt 2): 104-9. Abstract only. [cited by applicant]
Bhasin et al., Identification of a gene essential for O-acetylation of the [cited by applicant]
Bobrov et al., Insights into Yersinia pestis biofilm development: topology and co-interaction of Hms inner membrane proteins involved in exopolysaccharide production. Environ Microbiol. Jun. 2008;10(6):1419-32. doi: 10.… [cited by applicant]
Brown et al., Tolerance to single, but not multiple, amino acid replacements in antibody V-H CDR2: A means of minimizing B cell wastage from somatic hypermutation? J Immunol. Jan. 1, 1996;156(9): 3285-3291. [cited by applicant]
Burgeot et al., Immunopotentiation of [cited by applicant]
Capek et al., Chapters 22: Carbohydrates and Chapter 23: Polysaccharides. In: Journal of Chromatography Journal Library, vol. 3: Liquid column Chromatography, A Survey of Modern Technicques and Applications. Deyl et al.… [cited by applicant]
Cerca et al., Comparative antibody-mediated phagocytosis of [cited by applicant]
Cerca et al., Influence of batch or fed-batch growth on Staphylococcus epidermidis biofilm formation. Lett Appl Microbiol. 2004;39(5):420-4. [cited by applicant]
Cerca et al., Molecular basis for preferential protective efficacy of antibodies directed to the poorly acetylated form of staphylococcal poly-N-acetyl-beta-(1-6)-glucosamine. Infect Immun. Jul. 2007;75(7):3406-13. Epub… [cited by applicant]
Cerca et al., Protection against [cited by applicant]
Chanter, Partial purification and characterization of two non K99 mannose-resistant haemagglutinins of [cited by applicant]
Chen et al., Characterization and biological properties of chemically deglycosylated human chorionic gonadotropin. Role of carbohydrate moieties in adenylate cyclase activation. J Biol Chem. Dec. 10, 1982;257(23):14446-… [cited by applicant]
Chiavolini et al., Animal models of [cited by applicant]
Chibba et al., Synthesis and evaluation of inhibitors of [cited by applicant]
Christensen et al., Adherence of slime-producing strains of [cited by applicant]
Chu et al., Preparation, characterization, and immunogenicity of conjugates composed of the O-specific polysaccharide of Shigella dysenteriae type 1 (Shiga's bacillus) bound to tetanus toxoid. Infect Immun. Dec. 1991;59… [cited by applicant]
Conlon et al., icaR encodes a transcriptional repressor involved in environmental regulation of ica operon expression and biofilm formation in [cited by applicant]
Conlon et al., Regulation of icaR gene expression in [cited by applicant]
Conover et al., The Bps polysaccharide of Bordetella pertussis promotes colonization and biofilm formation in the nose by functioning as an adhesin. Mol Microbiol. Sep. 2010;77(6):1439-55. doi: 10.1111/j.1365-2958.2010.… [cited by applicant]
Cramton et al., Anaerobic conditions induce expression of polysaccharide intercellular adhesin in [cited by applicant]
Cramton et al., The intercellular adhesion (ica) locus is present in [cited by applicant]
Cywes-Bentley et al., Antibody to a conserved antigenic target is protective against diverse prokaryotic and eukaryotic pathogens. Proc Natl Acad Sci U S A. Jun. 11, 2013;110(24):E2209-18. doi: 10.1073/pnas.1303573110. … [cited by applicant]
Cywes-Bentley et al., Supporting Information for Antibody to a conserved antigenic target is protective against diverse prokaryotic and eukaryotic pathogens. Proc Natl Acad Sci U S A. Jun. 11, 2013;110(24):E2209-18. doi… [cited by applicant]
De Velasco et al., Anti-polysaccharide immunoglobulin isotype levels and opsonic activity of antisera: relationships with protection against [cited by applicant]
Depascalis et al., Grafting of “abbreviated” complementarity-determining regions containing specificity-determining residues essential for ligand contact to engineer a less immunogenic humanized monoclonal antibody. J I… [cited by applicant]
Dobinsky et al., Influence of Tn917 insertion on transcription of the icaADBC operon in six biofilm-negative transposon mutants of [cited by applicant]
Dobrin, et al., The role of complement, immunoglobulin and bacterial antigen in coagulase-negative staphylococcal shunt nephritis. Am J Med. Nov. 1975;59(5):660-73. Abstract only. [cited by applicant]
Elder et al., Characterization of monoclonal antibodies specific for adhesion: isolation of an adhesin of [cited by applicant]
Espersen, et al., Enzyme-linked immunosorbent assay for detection of [cited by applicant]
Espersen, et al., Solid-phase radioimmunoassay for IgG antibodies to [cited by applicant]
Fattom et al., Antigenic determinants of [cited by applicant]
Fattom et al., Comparative immunogenicity of conjugates composed of the [cited by applicant]
Fattom et al., Development of StaphVAX, a polysaccharide conjugate vaccine against [cited by applicant]
Fattom et al., Effect of conjugation methodology, carrier protein, and adjuvants on the immune response to [cited by applicant]
Fattom et al., Synthesis and immunologic properties in mice of vaccines composed of [cited by applicant]
Ferreiros et al., Purification and partial characterization of a K99-antigen associated adhesin in [cited by applicant]
Fey et al., Characterization of the relationship between polysaccharide intercellular adhesin and hemagglutination in [cited by applicant]
Fitzpatrick et al., Environmental regulation of biofilm formation in intensive care unit isolates of [cited by applicant]
Fournier et al., Purification and characterization of [cited by applicant]
Fowler et al., The intercellular adhesin locus ica is present in clinical isolates of [cited by applicant]
Frebourg et al., PCR-Based assay for discrimination between invasive and contaminating [cited by applicant]
Fridman et al., One-pot synthesis of glucosamine oligosaccharides. Org Lett. Jan. 24, 2002;4(2):281-3. [cited by applicant]
Gehron et al., Determination of the gram-positive bacterial content of soils and sediments by analysis of teichoic acid components. J Microbiol Methods. 1984;2:165-76. [cited by applicant]
Gelosia et al., Phenotypic and genotypic markers of [cited by applicant]
Gening et al., Synthesis of beta-(1-->6)-linked glucosamine oligosaccharides corresponding to fragments of the bacterial surface polysaccharide poly-N-acetylglucosamine. Carbohydr Res. Feb. 26, 2007;342(3-4):567-75. Epu… [cited by applicant]
Gening et al., Synthetic {beta}-(1->6)-linked N-acetylated and nonacetylated oligoglucosamines used to produce conjugate vaccines for bacterial pathogens. Infect Immun. Feb. 2010;78(2):764-72. Epub Nov. 30, 2009. [cited by applicant]
Gening et al., The study of the reaction of terminated oligomerization in the synthesis of oligo-(beta1-6)-glucosamines. Bioorg Khim. Jul.-Aug. 2006;32(4):432-43. Published in English in Russian Journal of Biorganic Che… [cited by applicant]
Gerke et al., Characterization of the N-acetylglucosaminyltransferase activity involved in the biosynthesis of the [cited by applicant]
Gerke et al., Experimental Pseudomonas aeruginosa Infection of the Mouse Cornea. Infect Immun. Feb. 1971;3(2):209-16. [cited by applicant]
Götz, [cited by applicant]
Grachev et al., NMR and conformational studies of linear and cyclic oligo-(1 →6)-β-D-glucosamines. Carbohydr Res. Nov. 8, 2011;346(15):2499-510. Epub Sep. 5, 2011. [cited by applicant]
Gray et al., Effect of extracellular slime substance from [cited by applicant]
Heilmann et al., Characterization of Tn917 insertion mutants of [cited by applicant]
Heilmann et al., Further characterization of [cited by applicant]
Heilmann et al., Molecular basis of intercellular adhesion in the biofilm-forming [cited by applicant]
Hermanson, Bioconjugate Techniques. Academic Press. 1996:34, 187-248. [cited by applicant]
Hinnebusch et al., Yersinia pestis Biofilm in the Flea Vector and Its Role in the Transmission of Plague. Curr Top Microbiol Immunol. 2008; 322: 229-48. [cited by applicant]
Hogt et al., Cell surface characteristics of coagulase-negative staphylococci and their adherence to fluorinated poly(ethylenepropylene). Infect Immun. Jan. 1986;51(1):294-301. [cited by applicant]
Huang et al., Risk of methicillin-resistant [cited by applicant]
Ichiman et al., Induction of resistance with heat-killed unencapsulated strains of [cited by applicant]
Ichiman et al., Relation of human serum antibody against [cited by applicant]
Ichiman et al., Specificity of monoclonal antibodies against an encapsulated strain of [cited by applicant]
Ichiman et al., The relationship of capsular-type of [cited by applicant]
Jabbouri et al., Characteristics of the biofilm matrix and its role as a possible target for the detection and eradication of [cited by applicant]
Jacques et al., Chemoenzymatic synthesis of GM3 and GM2 gangliosides containing a truncated ceramide functionalized for glycoconjugate synthesis and solid phase applications. Org Biomol Chem. Jan. 7, 2006;4(1):142-54. E… [cited by applicant]
Jefferson et al., Identification of a 5-nucleotide sequence that controls expression of the ica locus in [cited by applicant]
Jefferson et al., The teicoplanin-associated locus regulator (TcaR) and the intercellular adhesin locus regulator (IcaR) are transcriptional inhibitors of the ica locus in [cited by applicant]
Ji et al., Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science. Sep. 21, 2001;293(5538):2266-9. [cited by applicant]
Ji et al., Regulated antisense RNA eliminates alpha-toxin virulence in [cited by applicant]
Johnson et al., Interference with granulocyte function by [cited by applicant]
Jones, Revised structures for the capsular polysaccharides from [cited by applicant]
Joyce et al., Isolation, structural characterization, and immunological evaluation of a high-molecular-weight exopolysaccharide from [cited by applicant]
Kaplan et al., Genes involved in the synthesis and degradation of matrix polysaccharide in Actinobacillus actinomycetemcomitans and Actinobacillus pleuropneumoniae biofilms. J Bacteriol. Dec. 2004;186(24):8213-20. [cited by applicant]
Kelly-Quintos et al., Biological Characterization of Fully Human Monoclonal Antibodies to Staphylococcal Surface Polysaccharide PNAG. Abstracts of the 104th General Meeting of the American Society for Microbiology. Am S… [cited by applicant]
Kelly-Quintos et al., Characterization of the opsonic and protective activity against [cited by applicant]
Kelly-Quintos et al., The role of epitope specificity in the human opsonic antibody response to the staphylococcal surface polysaccharide poly N-acetyl glucosamine. J Infect Dis. Dec. 1, 2005;192(11):2012-9. Epub Nov. 1… [cited by applicant]
Keutmann et al., Evidence for a conformational change in deglycosylated glycoprotein hormones. FEBS Lett. Jun. 17, 1985;185(2):333-8. [cited by applicant]
Kille et al., Sucralose: assessment of teratogenic potential in the rat and the rabbit. Food Chem Toxicol. 2000;38 Suppl 2:S43-52. [cited by applicant]
Klevens et al., Invasive methicillin-resistant [cited by applicant]
Kohler, Derivation and diversification of monoclonal antibodies. Science. Sep. 19, 1986;233(4770):1281-6. [cited by applicant]
Kojima et al., Antibody to the capsular polysaccharide/adhesin protects rabbits against catheter-related bacteremia due to coagulase-negative staphylococci. J Infect Dis. Aug. 1990; 162(2):435-41. [cited by applicant]
Kolberg et al., Monoclonal antibodies with specificities for [cited by applicant]
Kossaczka et al., Synthesis and immunological properties of Vi and di-O-acetyl pectin protein conjugates with adipic acid dihydrazide as the linker. Infect Immun. Jun. 1997;65(6):2088-93. [cited by applicant]
Kropec et al., Poly-N-acetylglucosamine production in [cited by applicant]
Kuehnert et al., Methicillin-resistant- [cited by applicant]
Kuroda et al., Whole genome sequencing of meticillin-resistant [cited by applicant]
Lee et al., Chemical characterization and immunogenicity of capsular polysaccharide isolated from mucoid [cited by applicant]
Lee et al., Effect of a trivalent vaccine against [cited by applicant]
Lee et al., Protective efficacy of antibodies to the [cited by applicant]
Leith et al., Purification of a Mycoplasma pneumoniae adhesin by monoclonal antibody affinity chromatography. J Bacteriol. Feb. 1984;157(2):678-80. [cited by applicant]
Leung et al., Efficient synthesis and protein conjugation of beta-(1-->6)-D-N-acetylglucosamine oligosaccharides from the polysaccharide intercellular adhesin. Carbohydr Res. Mar. 31, 2009;344(5):570-5. Epub Jan. 3, 200… [cited by applicant]
Locksley, Chapter 94: Staphylococcal Infections. In Harrison's Principles of Internal Medicine, Eleventh Edition. Braunwald et al., eds. McGraw-Hill Book Company, Inc.: New York, 1950. p. 537-43. [cited by applicant]
Longworth et al., O-Acetylation status of the capsular polysaccharides of serogroup Y and W135 meningococci isolated in the UK. FEMS Immunol Med Microbiol. Jan. 14, 2002;32(2):119-23. Abstract Only. [cited by applicant]
Ludwicka et al., Investigation on extracellular slime substance produced by [cited by applicant]
MacCallum et al., Antibody-antigen interactions: contact analysis and binding site topography. J Mol Biol. Oct. 11, 1996;262(5):732-45. [cited by applicant]
Mack et al., Association of biofilm production of coagulase-negative staphylococci with expression of a specific polysaccharide intercellular adhesin. J Infect Dis. Oct. 1996; 174(4):881-4. [cited by applicant]
Mack et al., Characterization of transposon mutants of biofilm-producing [cited by applicant]
Mack et al., Essential functional role of the polysaccharide intercellular adhesin of [cited by applicant]
Mack et al., Genetic and biochemical analysis of [cited by applicant]
Mack et al., Identification of three essential regulatory gene loci governing expression of [cited by applicant]
Mack et al., Molecular mechanisms of [cited by applicant]
Mack et al., Parallel induction by glucose of adherence and a polysaccharide antigen specific for plastic-adherent [cited by applicant]
Mack et al., The intercellular adhesin involved in biofilm accumulation of [cited by applicant]
Maira-Litran et al., Biologic properties and vaccine potential of the staphylococcal poly-N-acetyl glucosamine surface polysaccharide. Vaccine. Feb. 17, 2004;22(7):872-9. Review. [cited by applicant]
Maira-Litrán et al., Comparative opsonic and protective activities of [cited by applicant]
Maira-Litran et al., Deacetylated-poly-N-acetyl Glucosamine (dPNAG) Polysaccharide Conjugated to Diphtheria Toxoid (DT) Confers Protection Against Multiple Strains of [cited by applicant]
Maira-Litran et al., Immunochemical properties of the staphylococcal poly-N-acetylglucosamine surface polysaccharide. Infect Immun. Aug. 2002;70(8):4433-40. [cited by applicant]
Maira-Litran et al., Relationship Between the Polysacharide Intercellular Adhesin (PIA) and Poly-N-Succinyl b-1-6 Glucosamine (PNSG) Molecules Produced by Pathogenic Staphylococi. In: Abstracts of the General Meeting of… [cited by applicant]
Maira-Litran et al., Synthesis and Immunological Properties of a Staphylococcal Deacetylated-poly-N-acetyl Glucosamine (dPNAG) Polysaccharide and Clumping Factor A (CIfA) Protein Conjugate Vaccine. Abstracts of the 104t… [cited by applicant]
McKenney et al., Broadly protective vaccine for [cited by applicant]
Mckenney et al., The ica locus of [cited by applicant]
Mckenney et al., Vaccine potential of poly-1-6 beta-D-N-succinylglucosamine, an immunoprotective surface polysaccharide of [cited by applicant]
McNeely et al., Antibody responses to capsular polysaccharide backbone and O-acetate side groups of [cited by applicant]
Melean et al., Toward the automated solid-phase synthesis of oligoglucosamines: systematic evaluation of glycosyl phosphate and glycosyl trichloroacetimidate building blocks. Carbohydr Res. Nov. 19, 2002;337(21-23):1893… [cited by applicant]
Michon et al., Structure activity studies on group C meningococcal polysaccharide-protein conjugate vaccines: effect of O-acetylation on the nature of the protective epitope. Dev Biol (Basel). 2000;103:151-60. Abstract … [cited by applicant]
Mikayama et al., Molecular cloning and functional expression of a cDNA encoding glycosylation-inhibiting factor. Proc Natl Acad Sci U S A. Nov. 1, 1993;90(21):10056-60. [cited by applicant]
Milstein, From antibody structure to immunological diversification of immune response. Science. Mar. 14, 1986;231(4743):1261-8. [cited by applicant]
Moch et al., Isolation and characterization of the alpha-sialyl-beta-2,3-galactosyl-specific adhesin from fimbriated [cited by applicant]
Moreau et al., Structure of the type 5 capsular polysaccharide of [cited by applicant]
Muller et al., Capsular polysaccharide/adhesin (PS/A) production by coagulase-negative staphylococci (CNS) is associated with adherence to silastic tubing. 1989:49. Abstract B-111. [cited by applicant]
Muller et al., Occurrence of capsular polysaccharide/adhesin among clinical isolates of coagulase-negative staphylococci. J Infect Dis. Nov. 1993;168(5):1211-8. [cited by applicant]
Nagy et al., Multi-adhesin vaccines for the protection of the neonatal piglet against “ [cited by applicant]
Nagy et al., Recombinant Antibodies for Infectious Diseases, Advances in Experimental Medicine and Biology. Chapter 7. Springer Nature. 2017;1053:119-153. Doi: https://doi.org/10.1007/978-3-319-72077-7_7. [cited by applicant]
Nakano, et al., Polyclonal antibody production in murine spleen cells induced by [cited by applicant]
O'Brien et al., Production of antibodies to [cited by applicant]
O'Gara et al., [cited by applicant]
Ohlsen et al., Immunotherapeutic strategies to combat staphylococcal infections. Int J Med Microbiol. Aug. 2010;300(6):402-10. Doi: 10.1016/j.ijmm.2010.04.015. Epub May 23, 2010. [cited by applicant]
Ohshima et al., Cell surface antigen of encapsulated [cited by applicant]
Ohshima et al., Immunochemical characterization and biological properties of a cell surface antigen extracted from encapsulated [cited by applicant]
Ohshima et al., Protection inducing antigen of an encapsulated [cited by applicant]
Orskov et al., An adhesive protein capsule of [cited by applicant]
Parise et al., Role of a putative polysaccharide locus in Bordetella biofilm development. J Bacteriol. Feb. 2007;189(3):750-60. [cited by applicant]
Pavliak, Carb 40- [cited by applicant]
Peters et al., Biology of [cited by applicant]
Peterson et al., The key role of peptidoglycan in the opsonization of [cited by applicant]
Pier et al., Further purification and characterization of high-molecular-weight polysaccharide from Pseudomonas aeruginosa. Infect Immun. Dec. 1983;42(3):936-41. [cited by applicant]
Pier et al., Isolation and characterization of a high-molecular-weight polysaccharide from the slime of Pseudomonas aeruginosa. Infect Immun. Dec. 1978;22(3):908-18. [cited by applicant]
Pier et al., Protective immunity induced in mice by immunization with high-molecular-weight polysaccharide from Pseudomonas aeruginosa. Infect Immun. Dec. 1978;22(3):919-25. [cited by applicant]
Pollack et al., Functional properties of isotype-switched immunoglobulin M (IgM) and IgG monoclonal antibodies to Pseudomonas aeruginosa lipopolysaccharide. Infect Immun. Nov. 1995;63(11):4481-8. [cited by applicant]
Posner et al., Epstein Barr virus transformation of peripheral blood B cells secreting antibodies reactive with cell surface antigens. Autoimmunity. 1990;8(2):149-58. [cited by applicant]
Pozzi et al., Opsonic and protective properties of antibodies raised to conjugate vaccines targeting six [cited by applicant]
Preston et al., Production and characterization of a set of mouse-human chimeric immunoglobulin G (IgG) subclass and IgA monoclonal antibodies with identical variable regions specific for [cited by applicant]
Preston et al., Prophylactic and therapeutic efficacy of immunoglobulin G antibodies to [cited by applicant]
Propst et al., Abstracts of the General Meeting of the American Society for Microbiology. 1998;42:242. Abstract only. 2 pages. [cited by applicant]
Quie et al., Coagulase-negative staphylococcal adherence and persistence. J Infect Dis. Oct. 1987;156(4):543-7. [cited by applicant]
Rogemond et al., Lectinlike adhesins in the Bacteroides fragilis group. Infect Immun. Jul. 1986;53(1):99-102. [cited by applicant]
Rohde et al., Structure, function and contribution of polysaccharide intercellular adhesin (PIA) to [cited by applicant]
Roux et al., Magic bullets for the 21st century: the reemergence of immunotherapy for multi- and pan-resistant microbes. J Antimicrob Chemother. Dec. 2012;67(12):2785-7. doi: 10.1093/jac/dks335. Epub Aug. 16, 2012. [cited by applicant]
Rudikoff et al., Single amino acid substitution altering antigen-binding specificity. Proc Natl Acad Sci U S A. Mar. 1982;79(6):1979-83. [cited by applicant]
Rupp et al., Characterization of [cited by applicant]
Rupp et al., Characterization of the importance of polysaccharide intercellular adhesin/hemagglutinin of [cited by applicant]
Sack, Deadly Bacteria Found to Be More Common. New York Times. Oct. 17, 2007. Available at: http://www.nytimes.com/2007/10/17/health/17infect.html?pagewanted=prin. Last accessed Apr. 30, 2010. 3 pages. [cited by applicant]
Sadovskaya et al., Potential use of poly-N-acetyl-beta-(1,6)-glucosamine as an antigen for diagnosis of staphylococcal orthopedic-prosthesis-related infections. Clin Vaccine Immunol. Dec. 2007;14(12):1609-15. Epub Oct. … [cited by applicant]
Sanford et al., Detection of staphylococcal membrane receptors on virus-infected cells by direct adhesin overlay. Infect Immun. Jun. 1986;52(3):671-5. [cited by applicant]
Sanger et al., A microapparatus for liquid hydrogen fluoride solvolysis: sugar and amino sugar composition of Erysiphe graminis and Triticum aestivum cell walls. Anal Biochem. Jan. 1983;128(1):66-70. [cited by applicant]
Schumacher-Perdreau et al., Comparative analysis of a biofilm-forming [cited by applicant]
Skurnik et al., Animal and human antibodies to distinct [cited by applicant]
Skurnik et al., Targeting pan-resistant bacteria with antibodies to a broadly conserved surface polysaccharide expressed during infection. J Infect Dis. Jun. 2012;205(11):1709-18. Doi: 10.1093/infdis/jis254. Epub Mar. 2… [cited by applicant]
Skurnik et al., The exceptionally broad-based potential of active and passive vaccination targeting the conserved microbial surface polysaccharide PNAG. Expert Rev Vaccines. Aug. 2016;15(8):1041-53. doi: 10.1586/1476058… [cited by applicant]
Soell et al., Capsular polysaccharide types 5 and 8 of [cited by applicant]
Soliman et al., Structural basis for antibody targeting of the broadly expressed microbial polysaccharide poly-N-acetylglucosamine. J Biol Chem. Apr. 6, 2018;293(14):5079-5089. doi: 10.1074/jbc.RA117.001170. Epub Feb. 1… [cited by applicant]
Sompolinsky et al., Encapsulation and capsular types in isolates of [cited by applicant]
Sundgren et al., Varied presentation of the Thomsen-Friedenreich disaccharide tumor-associated carbohydrate antigen on gold nanoparticles. Carbohydr Res. Jul. 21, 2008;343(10-11):1594-604. doi: 10.106/j.carres.2008.05.0… [cited by applicant]
Takeda et al., Protection against endocarditis due to [cited by applicant]
Tamura et al., Structural correlates of an anticarcinoma antibody: identification of specificity-determining residues (SDRs) and development of a minimally immunogenic antibody variant by retention of SDRs only. J Immun… [cited by applicant]
Thoma et al., Novel glycodendrimers self-assemble to nanoparticles which function as polyvalent ligands in vitro and in vivo. Angew Chem Int Ed Engl. Sep. 2, 2002;41(17):3195-8. [cited by applicant]
Thoma et al., Synthesis of oligosaccharide-polylysine conjugates: A well characterized sialyl lewis polymer for elisa. J Am Chem Soc. 1997;119(31):7414-5. [cited by applicant]
Thomas et al., Enzyme-linked lectinsorbent assay measures N-acetyl-D-glucosamine in matrix of biofilm produced by [cited by applicant]
Tojo et al., Isolation and characterization of a capsular polysaccharide adhesin from [cited by applicant]
Tollersrud et al., Genetic and serologic evaluation of capsule production by bovine mammary isolates of [cited by applicant]
Vershigora et al., Secretory antibodies to homologous and heterologous staphylococcal strains in the colostrum of rabbits. Zh Mikrobiol Epidemiol Immunobiol. 1980;88-90. Russian. 1 page. [cited by applicant]
Vlock et al., Abstracts of the 50th Interscience Conference on Antimicrobial Agents and Chemotherapy, Boston, MA, USA. Washington, DC, USA, American Society for Microbiology, Abstract G1-1654, p. 329, Sep. 12-15, 2010. [cited by applicant]
Von Eiff et al., Distribution of capsular and surface polysaccharide serotypes of [cited by applicant]
Vuong et al., A crucial role for exopolysaccharide modification in bacterial biofilm formation, immune evasion, and virulence. J Biol Chem. Dec. 24, 2004;279(52):54881-6. Epub Oct. 22, 2004. [cited by applicant]
Wang et al., The pgaABCD locus of [cited by applicant]
Wessels et al., Isolation and characterization of type IV group B [cited by applicant]
Wessels et al., Structural properties of group B streptococcal type III polysaccharide conjugate vaccines that influence immunogenicity and efficacy. Infect Immun. May 1998;66(5):2186-92. [cited by applicant]
Wicken et al., Characterization of group N [cited by applicant]
Wray et al., Identification and characterization of a uroepithelial cell adhesin from a uropathogenic isolate of Proteus mirabilis. Infect Immun. Oct. 1986;54(1):43-9. [cited by applicant]
Wright et al., Preparation of synthetic glycoconjugates as potential vaccines against Shigella flexneri serotype 2a disease. Org Biomol Chem. May 21, 2004;2(10):1518-27. Epub Apr. 26, 2004. [cited by applicant]
Yamada, et al., Possible common biological and immunological properties for detecting encapsulated strains of [cited by applicant]
Yang et al., A practical synthesis of a (1-->6)-linked beta-D-glucosamine nonasaccharide. Carbohydr Res. Mar. 14, 2003;338(6):495-502. [cited by applicant]
Yang et al., Synthesis of (1-6)-β-D-glucosamine hexasaccharide, a potential antimumor and immunostimulating agent. Tetrahedron Lett. Oct. 2002;43:7561-3. [cited by applicant]
Yang et al., Synthesis of biantennary beta-D-(1-->6) glucosamine oligosaccharides. Carbohydr Res. Jun. 16, 2003;338(12):1313-8. [cited by applicant]
Yoshida et al., Cross protection between an encapsulated strain of [cited by applicant]
Yoshida et al., Cross protection between a strain of [cited by applicant]