Adeno-associated virus (AAV) serotype 8 sequences, vectors containing same, and uses therefor
Sequences of a serotype 8 adeno-associated virus and vectors and host cells containing these sequences are provided. Also described are methods of using such host cells and vectors in production of rAAV particles.
1. A cultured host cell containing a recombinant nucleic acid molecule encoding an AAV vp1 capsid protein having a sequence comprising amino acids 1 to 738 of SEQ ID NO: 2 (AAV8) or a sequence at least 95% identical to the full length of amino acids 1 to 738 of SEQ ID NO: 2, wherein the recombinant nucleic acid molecule further comprises a heterologous non-AAV sequence.
2. The cultured host cell according to claim 1 , which further comprises a functional rep gene.
3. A cultured host cell containing a recombinant nucleic acid molecule encoding an AAV vp2 capsid protein having a sequence comprising amino acids 138 to 738 of SEQ ID NO: 2 (AAV8) or a sequence at least 95% identical to the full length of amino acids 138 to 738 of SEQ ID NO: 2, wherein the recombinant nucleic acid molecule further comprises a heterologous non-AAV sequence.
4. The cultured host cell according to claim 3 , which further comprises a functional rep gene.
5. A cultured host cell containing a recombinant nucleic acid molecule encoding an AAV vp3 capsid protein having a sequence comprising amino acids 204 to 738 of SEQ ID NO: 2 (AAV8) or a sequence at least 95% identical to the full length of amino acids 204 to 738 of SEQ ID NO: 2, wherein the recombinant nucleic acid molecule further comprises a heterologous non-AAV sequence.
6. The cultured host cell according to claim 5 , which further comprises a functional rep gene.
7. A cultured host cell containing a recombinant nucleic acid molecule comprising
(a) nucleotides 2121 to 4334 of SEQ ID NO: 1 or a sequence at least 95% identical to nucleotides 2121 to 4334 of SEQ ID NO: 1,
(b) nucleotides 2532 to 4334 of SEQ ID NO: 1 or a sequence at least 95% identical to nucleotides 2532 to 4334 of SEQ ID NO: 1, or
(c) nucleotides 2730 to 4334 of SEQ ID NO: 1 or a sequence at least 95% identical to nucleotides 2730 to 4334 of SEQ ID NO: 1,
wherein the recombinant nucleic acid molecule further comprises a heterologous non-AAV sequence.
8. The cultured host cell according to claim 7 , which further comprises a rep gene.
9. The cultured host cell according to claim 7 , wherein said nucleotide sequence is at least 95% identical to nucleotides 2121 to 4334 of SEQ ID NO: 1.
10. The cultured host cell according to claim 7 , wherein said nucleotide sequence is at least 98% identical to nucleotides 2121 to 4334 of SEQ ID NO: 1.
11. The cultured host cell according to claim 7 , wherein said nucleotide sequence is at least 99% identical to nucleotides 2121 to 4334 of SEQ ID NO: 1.
12. The cultured host cell according to claim 7 , wherein said nucleotide sequence is at least 95% identical to nucleotides 2532 to 4334 of SEQ ID NO: 1.
13. The cultured host cell according to claim 7 , wherein said nucleotide sequence is at least 98% identical to nucleotides 2532 to 4334 of SEQ ID NO: 1.
14. The cultured host cell according to claim 7 , wherein said nucleotide sequence is at least 99% identical to nucleotides 2532 to 4334 of SEQ ID NO: 1.
15. The cultured host cell according to claim 7 , wherein said nucleotide sequence is at least 95% identical to nucleotides 2730 to 4334 of SEQ ID NO: 1.
16. The cultured host cell according to claim 7 , wherein said nucleotide sequence is at least 98% identical to nucleotides 2730 to 4334 of SEQ ID NO: 1.
17. The cultured host cell according to claim 7 , wherein said nucleotide sequence is at least 99% identical to nucleotides 2730 to 4334 of SEQ ID NO: 1.
18. The cultured host cell according to claim 2 , wherein the rep gene is from AAV2.
19. The cultured host cell according to claim 4 , wherein the rep gene is from AAV2.
20. The cultured host cell according to claim 6 , wherein the rep gene is from AAV2.
21. The cultured host cell according to claim 8 , wherein the rep gene is from AAV2.
22. The cultured host cell according to claim 1 , wherein the recombinant nucleic acid molecule is a plasmid.
23. The cultured host cell according to claim 3 , wherein the recombinant nucleic acid molecule is a plasmid.
24. The cultured host cell according to claim 5 , wherein the recombinant nucleic acid molecule is a plasmid.
25. The cultured host cell according to claim 7 , wherein the recombinant nucleic acid molecule is a plasmid.