Chromatographic data system processing apparatus
A chromatographic data system processing apparatus includes a liquid feeder, a sample injector, a column that separates samples, a detector, a controller that processes a detected result of the detector, and a data processor that examines and sets operations of the liquid feeder, the column and the detector, and a measurement condition. The data processor generates a three-dimensional graph having three axes related to a pressure, a time, and a number of theoretical plates based on data or variables indicating a relationship between the number of theoretical plates and a flow rate, and data or variables indicating a relationship between the pressure and the flow rate. The chromatographic data system processing apparatus can easily obtain a separation condition for obtaining performance from a three-dimensional graph including a pressure drop, a hold-up time and a number of theoretical plates.
1. A chromatographic data system processing apparatus, comprising:
a liquid feeder configured to feed a mobile phase;
a sample injector configured to inject a sample into a mobile phase flowing path into which the mobile phase is fed;
a column configured to separate analytes from the injected sample;
a detector configured to detect the separated analytes;
a controller programmed to process a detected result of the detector; and
a data processor programmed to examine and set operations of the liquid feeder, the column and the detector, and a measurement condition, wherein the data processor generates a three-dimensional graph having three axes related to a pressure, a time, and a number of theoretical plates based on data or variables indicating a relationship between the number of theoretical plates and a flow rate, and data or variables indicating a relationship between the pressure and the flow rate to analyze a separation condition from the generated three-dimensional graph.
2. The chromatographic data system processing apparatus according to claim 1 ,
wherein the data processor axially transforms at least one of the pressure and the time related to two axes out of the three axes to at least one of the flow rate and a length.
3. The chromatographic data system processing apparatus according to claim 1 ,
wherein the data processor represents at least one axis out of the three axes using a logarithm.
4. The chromatographic data system processing apparatus according to claim 3 ,
wherein the data processor transforms an axis in logarithmic representation to an axis in antilogarithm representation.
5. The chromatographic data system processing apparatus according to claim 1 ,
wherein the data processor transforms the three-dimensional graph having the three axes related to the pressure, the time and the number of theoretical plates to a different three-dimensional graph of three axes related to the flow rate, a length and the number of theoretical plates.
6. The chromatographic data system processing apparatus according to claim 1 ,
wherein the data processor transforms a two-dimensional graph having two axes related to the pressure and the time out of the three axes to a different two-dimensional graph having two axes related to the flow rate and a length.
7. The chromatographic data system processing apparatus according to claim 1 ,
wherein the data processor selects arbitrary two variables from four variables related to the pressure, the time, the flow rate and a length, and transforms a two-dimensional graph having two axes related to the selected variables to a different two-dimensional graph having two axes related to two variables not selected.
8. The chromatographic data system processing apparatus according to claim 7 ,
wherein the data processor selects two variables from the four variables, and outputs a partial differential coefficient in a function such as the number of theoretical plates having the two selected variables as variables.
9. The chromatographic data system processing apparatus according to claim 8 ,
wherein the data processor outputs a dimensionless efficiency standardized with using the variable to be partially differentiated based on the partial differential coefficient.
10. The chromatographic data system processing apparatus according to claim 9 ,
wherein the data processor outputs a new efficiency by multiplication and division calculation with using two or more of the dimensionless efficiencies to be output.
11. The chromatographic data system processing apparatus according to claim 1 ,
wherein the data processor uses:
a linear velocity, or a reciprocal thereof as the flow rate;
a column length, or a reciprocal thereof as the length;
a column pressure drop, a velocity length product or pressure-driven strength, or a reciprocal thereof as the pressure;
a hold-up time or a retention time, or a reciprocal thereof as the time; and
a square theoretical plate number, a theoretical plate equivalent height, a square theoretical plate equivalent height, a resolution, a separation impedance, an impedance time or a plate time, or a reciprocal thereof as the number of theoretical plates.
12. A chromatographic data system processing apparatus, comprising:
a liquid feeder configured to feed a mobile phase;
a sample injector configured to inject a sample into a mobile phase flowing path into which the mobile phase is fed;
a column configured to separate the injected sample;
a detector configured to detect the separated analytes;
a controller configured to process a detected result of the detector; and
a data processor configured to examine and set operations of the liquid feeder, the column and the detector, and a measurement condition,
wherein, during a process of selecting two variables for axes from four variables related to a linear velocity, a length, a pressure and a time to analyze a separation condition, the data processor is programmed to transform the axes of the selected two variables into axes of two variables not selected.
13. A chromatographic data system processing apparatus that analyzes and processes data of an analysis condition and a detection result of a chromatograph,
wherein the chromatographic data system processing apparatus is programmed to output a three-dimensional graph having three axes related to a pressure, a time, and a number of theoretical plates based on data or variables indicating a relationship between the number of theoretical plates and a flow rate, and data or variables indicating a relationship between the pressure and the flow rate, and is thereby programmed to analyze a separation condition from the output three-dimensional graph.