DNA gridiron compositions and methods
View Patent ↗Novel compositions and methods for engineering wireframe architectures and scaffolds of increasing complexity by creating gridiron-like DNA structures (FIG. 1 ). A series of four-arm junctions are used as vertices within a network of double-helical DNA fragments. Deliberate distortion of the junctions from their most relaxed conformations ensures that a scaffold strand can traverse through individual vertices in multiple directions. DNA gridirons, ranging from two-dimensional arrays with reconfigurability to multilayer and three-dimensional structures and curved objects, can be assembled according the methods presented herein.
1. A composition comprising
a plurality of immobile Holliday junction analogs linked together in a layered square frame having at least two layers in which the DNA helices which lie on opposite sides of the Holliday junction also lie in the same plane, and wherein said plurality of immobile Holliday junction analogs are linked together with a central strand of single-stranded DNA within said square frame.
2. The composition of claim 1 , wherein said plurality of immobile Holliday junction analogs are linked together in a square frame having at least three layers.
3. The composition of claim 1 , wherein said plurality of immobile Holliday junction analogs are linked together in a square frame having at least four layers.
4. The composition of claim 1 , wherein said single stranded DNA comprises M13mp18 DNA.
5. The composition of claim 2 , wherein the square frame having at least three layers comprises a lattice defined by the lengths of said lattice, and the interstitial space confined by the lattice defines a cavity;
wherein the lattice comprises at least three double-stranded DNA strands, each of which has an independent length.
6. The composition of claim 5 , wherein the cavity size length of the double-stranded DNA strands is from 21 nt to 63 nt.
7. The composition of claim 3 , wherein the square frame having at least four layers, comprises a lattice defined by the lengths of said lattice, and the interstitial space confined by the lattice defines a cavity,
wherein the lattice comprises at least three separate lengths of double-stranded DNA.
8. The composition of claim 7 , wherein the length of the double-stranded DNA strands is from 21 nt to 63 nt.