Respiratory syncytial virus expression vectors
View Patent ↗In certain embodiments, the disclosure relates to vectors containing bacterial nucleic acid sequences and a paramyxovirus gene. Typically, the expression vector comprises a bacterial artificial chromosome (BAC), and a nucleic acid sequence comprising a respiratory syncytial virus (RSV) gene in operable combination with a regulatory element and optionally a reporter gene.
1. A nucleic acid comprising SEQ ID NO: 4.
2. A nucleic acid comprising SEQ ID NO: 5.
3. A nucleic acid comprising SEQ ID NO: 4 and SEQ ID NO: 5.
4. A vector comprising the nucleic acid of claim 3 .
5. The vector of claim 4 wherein the vector is a plasmid.
6. A cell comprising a vector of claim 4 .
7. A vector comprising a single multi-cloning site comprising the restriction sites BstBI, SacI, ClaI, AvrII, and MluI, in that order.
8. The vector of claim 7 wherein the vector is a plasmid.
9. A cell comprising a vector of claim 7 .