Polypeptides for use in self-assembling protein nanostructures
Synthetic nanostructures, polypeptides that are useful, for example, in making synthetic nanostructures, and methods for using such synthetic nanostructures are disclosed herein.
1. A nanostructure, comprising:
(a) a plurality of first assemblies, each first assembly comprising a plurality of identical first polypeptides, wherein the first polypeptides comprise an amino acid sequence that is at least 80% identical to, the amino acid sequence of a polypeptide selected from the group consisting of SEQ ID NOS:1-34 and identical at least at one half of identified interface positions;
Name
Amino Acid Sequence
I53-34A
MEGMDPLAVLAESRLLPLLTVRGGEDLAGLATVLELMGVG
SEQ ID
ALEITLRTEKGLEALKALRKSGLLLGAGTVRSPKEAEAAE
NO: 1
EAGAAFLVSPGLLEEVAALAQARGVPYLPGVLTPTEVERA
LALGLSALKFFPAEPFQGVRVLRAYAEVFPEVRFLPTGGI
KEEHLPHYAALPNLLAVGGSWLLQGDLAAVMKKVKAAKAL
LSPQAPG
I53-34B
MTKKVGIVDITFARVDMAEAAIRTLKALSPNIKIIRKTVP
SEQ ID
GIKDLPVACKKLLEEEGCDIVMALGMPGKAEKDKVCAHEA
NO: 2
SLGLMLAQLMTNKHIIEVFVHEDEAKDDDELDILALVRAI
EHAANVYYLLFKPEYLTRMAGKGLRQGREDAGPARE
I53-40A
MTKKVGIVDTTFARVDMASAAILTLKMESPNIKIIRKTVP
SEQ ID
GIKDLPVACKKLLEEEGCDIVMALGMPGKAEKDKVCAHEA
NO: 3
SLGLMLAQLMTNKHIIEVFVHEDEAKDDAELKILAARRAI
EHALNVYYLLFKPEYLTRMAGKGLRQGFEDAGPARE
I53-40B
MSTINNQLKALKVIPVIAIDNAEDIIPLGKVLAENGLPAA
SEQ ID
EITFRSSAAVKAIMLLRSAQPEMUGAGTILNGVQALAAKE
NO: 4
AGATFVVSPGFNPNTVRACQIIGIDIVPGVNNPSTVEAAL
EMGLTTLKFFPAEASGGISMVKSLVGPYGDIRLMPTGGIT
PSNIDNYLAIPQVLACGGTWMVDKKLVTNGEWDEIARLTR
EIVEQVNP
I53-47A
MPIFTLNTNIKATDVPSDFLSLTSRLVGLILSKPGSYVAV
SEQ ID
HINTDQQLSFGGSTNPAAFGTLMSIGGIEPSKNRDHSAVL
NO: 5
FDHENAMEGIPKNRMYIHFVNLNGDDVGWNGTTF
I53-47B
MNQHSHKDYETVRIAVVRARWHADIVDACVEAFEIAMAAI
SEQ ID
GGDRFAVDVFDVPGAYEIPLHARTFAETGRYGAVLGTAFV
NO: 6
VNGGIYRHEFVASAVIDGMMNVQLSTGVPVLSAVLTPHRY
RDSAEHHRFFAAHFAVKGVEAARACIEILAAREKIAA
I53-50A
MKMEELFKKHKIVAVLRANSVEEAIEKAVAVFAGGVHLIE
SEQ ID
ITFTVPDADTVIKALSVLKEKGAIIGAGTVTSVEQCRKAV
NO: 7
ESGAEFIVSPHLDEEISQFCKEKGVFYMPGVMTPTELVKA
MKLGHTILKLFPGEVVGPQFVKAMKGPFPNVKFVPTGGVN
LDNVCEWFKAGVEAVGVGSALVKGTPDEVREKAKAFVEKI
RGCTE
I53-50B
MNQHSHKDYETVRIAVVRARWHAEIVDACVSAFEAAMADI
SEQ ID
GGDRFAVDVFDVPGAYEIPLHARTLAETGRYGAVLGTAFV
NO: 8
VNGGIYRHEFVASAVIDGMMNVQLSTGVPVLSAVLTPHRY
RDSDAHTLLFLALFAVKGMEAARACVEILAAREKIAA
I53-51A
MFTKSGDDGNTNVINKRVGKDSPLVNFLGDLDELNSFIGF
SEQ ID
AISKIPWEDMKKDLERVQVELFEIGEDLSTQSSKKKIDES
NO: 9
YVLWLLAATAIYRIESGPVKLFVIPGGSEEASVLHVTRSV
ARRVERNAVKYTKEEPEINRMIIVYLNRLSSLLFAMALVA
NKRRNQSEKIYEIGKSW
I53-51B
MNQHSHKDYETVRIAVVRARWHADIVDQCVRAFEEAMADA
SEQ ID
GGDRFAVDVFDVPGAYEIPLHARTLAETGRYGAVLGTAFV
NO: 10
VNGGIYRHEFVASAVIDGMMNVQLSTGVPVLSAVLTPHRY
RSSREHHEFFREHFMVKGVEAAAACITILAAREKIAA
I52-03A
MGHTKGPTPQQHDGSALRIGIVHARWNKTIIMPLLIGTIA
SEQ ID
KLLECGVKASNIVVQSVPGSWELPIAVQRLYSASQLQTPS
NO: 11
SGPSLSAGDLLGSSTTDLTALPTTTASSTGPFDALIAIGV
EIKGETMFHEYIADSVSHGLMRVQLDTGVPVIFGVLTVLT
DDQAKARAGVIEGSHNHGEDWGLAAVEMGVRRRDWAAGKT
E
I52-03B
MYEVDHADVYDLFYLGRGKDYAAEASDIADLVRSRTPEAS
SEQ ID
SELDVACGTGTHLEMFTKEFGDTAGLELSEDMLTHARKRL
NO: 12
PDATLHQGDMRDFQLGRKFSAVVSMFSSVGYEKTVAELGA
AVASFAEHLEPGGVVVVEPWWFPETFADGWVSADVVRRDG
RTVARVSHSVREGNATRMEVHFTVADPGKGVRHFSDVHLI
TLFHQREYEAAFMAAGLRVEYLEGGPSGRGLFVGVPA
I52-32A
MGMKEKFVLIITHGDFGKGLLSGAEVIIGKQENVHTVGLN
SEQ ID
LGDNIEKVAKEVMRIIIAKLAEDKEIIIVVDLFGGSPFNI
NO: 13
ALEMMKTFDVKVITGINMPMLVELLTSINVYDTTELLENI
SKIGKDGIKVIEKSSLKM
I52-32B
MKYDGSKLRIGILHARWNLEIIAALVAGAIKRLQEFGVKA
SEQ ID
ENIIIETVPGSFELPYGSKLFVEKQKRLGKPLDAIIPIGV
NO: 14
LIKGSTMHFEYICDSTTHQLMKLNFELGIPVIFGVLTCLT
DEQAEARAGLIEGKMHNHGEDWGAAAVEMATKFN
I52-33A
MAVKGLGEVDQKYDGSKLRIGILHARWNRKIILALVAGAV
SEQ ID
LRLLEFGVKAENIIIETVPGSFELPYGSKLFVEKQKRLGK
NO: 15
PLDAIIPIGVLIKGSTMHFEYICDSTTHQLMKLNFELGIP
VIFGVLTCLTDEQAEARAGLIEGKMHNHGEDWGAAAVEMA
TKFN
I52-33B
MGANWYLDNESSRLSFTSTKNADIAEVHRFLVLHGKVDPK
SEQ ID
GLAEVEVETESISTGIPLRDMLLRVLVFQVSKFPVAQINA
NO: 16
QEDMRPINNLAPGAQLELRLPLTVSLRGKSHSYNAELLAT
RLDERRFQVVTLEPLVIHAQDFDMVRAFNALRLVAGLSAV
SLSVPVGAVLIFTAR
I32-06A
MTDYIRDGSAIKALSFAIILAEADLRHIPQDLQRLAVRVI
SEQ ID
HACGMVDVANDLAFSEGAGKAGRNALLAGAPILCDARMVA
NO: 17
EGITRSRLPADNRVIYTLSDPSVPELAKKIGNTRSAAALD
LWLPHIEGSIVAIGNAPTALFRLFELLDAGAPKPALIIGM
PVGFVGAAESKDELAANSRGVPYVIVRGRRGGSAMTAAAV
NALASERE
I32-06B
MITVFGLKSKLAPRREKLAEVIYSSLHLGLDIPKGKHAIR
SEQ ID
FLCLEKEDFYYPFDRSDDYTVIEINLMAGRSEETKMLLIF
NO: 18
LLFIALERKLGIRAHIDVEITIKEQPAHCWGFRGRTGDSA
RDLDYDIYV
I32-19A
MGSDLQKLQRFSTCDISDGLLNVYNIPTGGYFPNLTAISP
SEQ ID
PQNSSIVGTAYTVEFAPIDDPRPAVNYIDSVPPNSILVLA
NO: 19
LEPHLQSQFITPFIKITQAMYGGLMSTRAQYLKSNGTVVF
GRIRDVDEHRTLNHPVFAYGVGSCAPKAVVKAVGTNVQLK
ILTSDGVTQTICPGDYIAGDNNGIVRIPVQETDISKLVTY
IEKSIEVDRLVSEAIKNGLPAKAAQTARRMVLKDYI
I32-19B
MSGMRVYLGADHAGYELKQAIIAFLKMTGHEPIDCGALRY
SEQ ID
DADDDYPAFCIAAATRTVADPGSLGIVLGGSGNGEQIAAN
NO: 20
KVPGARCALAWSVQTAALAREHNNAQLIGIGGRMHTLEEA
LRIVKAFVTTPWSKAQRHQRRIDILAEYERTHEAPPVPGA
PA
I32-28A
MGDDARIAAIGDVDELNSQIGVLLAEPLPDDVRAALSAIQ
SEQ ID
HDLFDLGGELCIPGHAAITEDHLLRLALWLVHYNGQLPPL
NO: 21
EEFILPGGARGAALAHVCRTVCRRAERSIKALGASEPLNI
APAAYVNLLSDLLFVLARVLNRAAGGADVLWDRTRAH
I32-28B
MILSAEQSFTLRHPHGQAAALAFVREPAAALAGVQRLRGL
SEQ ID
DSDGEQVWGELLVRVPLLGEVDLPFRSEIVRTPQGAELRP
NO: 22
LTLTGERAWVAVSGQATAAEGGEMAFAFQFQAHLATPEAE
GEGGAAFEVMVQAAAGVTLLLVAMALPQGLAAGLPPA
I53-40A.1
MTKKVGIVDTTFARVDMASAAILTLKMESPNIKIIRKTVP
SEQ ID
GIKDLPVACKKLLEEEGCDIVMALGMPGKKEKDKVCAHEA
NO: 23
SLGLMLAQLMTNKHIIEVFVHEDEAKDDAELKILAARRAI
EHALNVYYLLFKPEYLTRMAGKGLRQGFEDAGPARE
I53-40B.1
MDDINNQLKRLKVIPVIAIDNAEDIIPLGKVLAENGLPAA
SEQ ID
EITFRSSAAVKAIMLLRSAQPEMLIGAGTILNGVQALAAK
NO: 24
EAGADFVVSPGFNPNTVRACQIIGIDIVPGVNNPSTVEQA
LEMGLTTLKFFPAEASGGISMVKSLVGPYGDIRLMPTGGI
TPDNIDNYLAIPQVLACGGTWMVDKKLVRNGEWDEIARLT
REIVEQVNP
I53-47A.1
MPIFTLNTNIKADDVPSDFLSLTSRLVGLILSKPGSYVAV
SEQ ID
HINTDQQLSFGGSTNPAAFGTLMSIGGIEPDKNRDHSAVL
NO: 25
FDHLNAMLGIPKNRMYIHFVNLNGDDVGWNGTTF
I53-47A.1
MPIFTLNTNIKADDVPSDFESLTSREVGLILSEPGSYVAV
NegT2
HINTDQQLSFGGSTNPAAFGTLMSIGGIEPDKNEDHSAVL
SEQ ID
FDHENAMLGIPKNRMYIHFVDLDGDDVGWNGTTF
NO: 26
I53-47B.1
MNQHSHKDHETVRIAVVRARWHADIVDACVEAFEIAMAAI
SEQ ID
GGDRFAVDVFDVPGAYEIPLHARTLAETGRYGAVLGTAFV
NO: 27
VNGGIYRHEFVASAVIDGMMNVQLDTGVPVLSAVLTPMRY
RDSDEHHRFFAAHAVKGVEAARACIEILNAREKIAA
I53-47B.1
MNQHSHKDHETVRIAVVRARWHADIVDACVEAFEIAMAAI
NegT2
GGDRFAVDVFDVPGAYEIPLHARTLAETGRYGAVLGTAFV
SEQ ID
VDGGIYDHEFVASAVIDGMMNVQLDTGVPVLSAVLTPHEY
NO: 28
EDSDEDHEFFAAHFAVKGVEAARACIELNAREKIAA
I53-50A.1
MKMEELFKKHKIVAVLRANSVEEAIEKAVAVFAGGVHLIE
SEQ ID
ITFTVPDADTVIKALSVLKEKGAIIGAGTVTSVEQCRKAV
NO: 29
ESGAEFIVSPHLDEEISQFCKEKGVFYMPGVMTPTELVKA
MKLGHDILKLFPGEVVGPQFVKAMKGPFPNVKFVPTGGVN
LDNVCEWFKAGVLAVGVGDALVKGDPDEVREKAKKFVEKI
RGCTE
I53-50A.1
MKMEELFKKHKIVAVLRANSVEEAIEKAVAVFAGGVHLIE
NegT2
ITFTVPDADTVIKALSVLKEKGAIIGAGTVTSVEQCRKAV
SEQ ID
ESGAEFIVSPHLDEEISQFCKEKGVFYMPGVMTPTELVKA
NO: 30
MKLGHDILKLFPGEVVGPEFVEAMKGPFPNVKFVPTGGVD
LDDVCEWFDAGVLAVGVGDALVEGDPDEVREDAKEFVEEI
RGCTE
I53-50A.1
MKMEELFKKHKIVAVLRANSVEEAIEKAVAVFAGGVHLIE
PosT1
ITFTVPDADTVIKALSVLKEKGAIIGAGTVTSVEQCRKAV
SEQ ID
ESGAEFIVSPHLDEEISQFCKEKGVFYMPGVMTPTELVKA
NO: 31
MKLGHDILKLFPGEVVGPQFVKAMKGPFPNVKFVPTGGVN
LDNVCKWFKAGVLAVGVGKALVKGKPDEVREKAKKFVKKI
RGCTE
I53-50B.1
MNQHSHKDHETVRIAVVRARWHAEIVDACVSAFEAAMRDI
SEQ ID
GGDRFAVDVFDVPGAYEIPLHARTLAETGRYGAVLGTAFV
NO: 32
VNGGIYRHEFVASAVIDGMMNVQLDTGVPVLSAVLTPHRY
RDSDAHTLLFLALFAVKGMEAARACVEILAAREKIAA
I53-50B.1
MNQHSHKDHETVRIAVVRARWHAEIVDACVSAFEAAMRDI
NegT2
GGDRFAVDVFDVPGAYEIPLHARTLAETGRYGAVLGTAFV
SEQ ID
VDGGIYDHEFVASAVIDGMMNVQLDTGVPVLSAVLTPHEY
NO: 33
EDSDADTLLFLALFAVKGMEAARACVEILAAREKIAA
I53-50B.4
MNQHSHKDHETVRIAVVRARWHAEIVDACVSAFEAAMRDI
PosT1
GGDRFAVDVFDVPGAYEIPLHARTLAETGRYGAVLGTAFV
SEQ ID
VNGGIYRHEFVASAVINGMMNVQLNTGVPVLSAVLTPHNY
NO: 34
DKSKAHTLLFLALFAVKGMEAARACVEILAAREKIAA
Name
Identified interface residues
I53-34A
I53-34A:
SEQ ID
28, 32, 36, 37, 186, 188, 191, 192,
NO: 1
195
I53-34B
I53-34B:
SEQ ID
19, 20, 23, 24, 27, 109, 113, 116, 117,
NO: 2
120, 124, 148
I53-40A
I53-40A:
SEQ ID
20, 23, 24, 27, 28, 109, 112, 113, 116,
NO: 3
120, 124
I53-40B
I53-40B:
SEQ ID
47, 51, 54, 58, 74, 102
NO: 4
I53-47A
I53-47A:
SEQ ID
22, 25, 29, 72, 79, 86, 87
NO: 5
I53-47B
I53-47B:
SEQ ID
28, 31, 35, 36, 39, 131, 132, 135,
NO: 6
139, 146
I53-50A
I53-50A:
SEQ ID
25, 29, 33, 54, 57
NO: 7
I53-50B
I53-50B:
SEQ ID
24, 28, 36, 124, 125, 127, 128, 129,
NO: 8
131, 132, 133, 135, 139
I53-51A
I53-51A:
SEQ ID
80, 83, 86, 87, 88, 90, 91, 94, 166,
NO: 9
172, 176
I53-51B
I53-51B:
SEQ ID
31, 35, 36, 40, 122, 124, 128, 131,
NO: 10
135, 139, 143, 146, 147
I52-03A
I52-03A:
SEQ ID
28, 32, 36, 39, 44, 49
NO: 11
I52-03B
I52-03B:
SEQ ID
94, 155, 116, 206, 213
NO: 12
I52-32A
I52-32A:
SEQ ID
47, 49, 53, 54, 57, 58, 61, 83, 87, 88
NO: 13
I52-32B
I52-32B:
SEQ ID
19, 20, 23, 30, 40
NO: 14
I52-33A
I52-33A:
SEQ ID
33, 41, 44, 50
NO: 15
I52-33B
I52-33B:
SEQ ID
61, 63, 66, 67, 72, 147, 148, 154,
NO: 16
155
I32-06A
I32-06A:
SEQ ID
9, 12, 13, 14, 20, 30, 33, 34
NO: 17
I32-06B
I32-06B:
SEQ ID
24, 71, 73, 76, 77, 80, 81, 84, 85,
NO: 18
88, 114, 118
I32-19A
I32-19A:
SEQ ID
208, 213, 218, 222, 225, 226, 229, 233
NO: 19
I32-19B
I32-19B:
SEQ ID
20, 23, 24, 27, 117, 118, 122, 125
NO: 20
I32-28A
I32-28A:
SEQ ID
60, 61, 64, 67, 68, 71, 110, 120, 123,
NO: 21
124, 128
I32-28B
I32-28B:
SEQ ID
35, 36, 54, 122, 129, 137, 140, 141,
NO: 22
144, 148
I53-40A.1
I53-40A:
SEQ ID
20, 23, 24, 27, 28, 109, 112, 113, 116,
NO: 23
120, 124
I53-40B.1
I53-40B:
SEQ ID
47, 51, 54, 58, 74, 102
NO: 24
I53-47A.1
I53-47A:
SEQ ID
22, 25, 29, 72, 79, 86, 87
NO: 25
I53-47A.1
I53-47A:
NegT2
22, 25, 29, 72, 79, 86, 87
SEQ ID
NO: 26
I53-47B.1
I53-47B:
SEQ ID
28, 31, 35, 36, 39, 131, 132, 135,
NO: 27
139, 146
I53-47B.1
I53-47B:
NegT2
28, 31, 35, 36, 39, 131, 132, 135,
SEQ ID
139, 146
NO: 28
I53-50A.1
I53-50A:
SEQ ID
25, 29, 33, 54, 57
NO: 29
I53-50A.1
I53-50A:
NegT2
25, 29, 33, 54, 57
SEQ ID
NO: 30
I53-50A.1
I53-50A:
PosT1
25, 29, 33, 54, 57
SEQ ID
NO: 31
I53-50B.1
I53-50B:
SEQ ID
24, 28, 36, 124, 125, 127, 128, 129,
NO: 32
131, 132, 133, 135, 139
I53-50B.1
I53-50B:
NegT2
24, 28, 36, 124, 125, 127, 128, 129,
SEQ ID
131, 132, 133, 135, 139
NO: 33
I53-50B.4
I53-50B:
PosT1
24, 28, 36, 124, 125, 127, 128, 129,
SEQ ID
131, 132, 133, 135, 139
NO: 34
and
(b) a plurality of second assemblies, each second assembly comprising a plurality of identical second polypeptides, wherein the second polypeptides comprise an amino acid sequence that is at least 80% identical to, the amino acid sequence of a polypeptide selected from the group consisting of SEQ ID NOS:1-34 and identical at least at one half of identified interface positions,
wherein the second polypeptide differs from the first polypeptide; and
wherein the plurality of first assemblies non-covalently interact with the plurality of second assemblies to form a nanostructure.
2. The nanostructure of claim 1 , wherein the nanostructure has icosahedral symmetry.
3. The nanostructure of claim 1 , wherein the first polypeptides and the second polypeptides comprise polypeptides with amino acid sequences at least 85% identical to the amino acid sequences selected from the following pairs:
(i) SEQ ID NO:1 and SEQ ID NO:2 (I53-34A and I53-34B);
(ii) SEQ ID NO:3 and SEQ ID NO:4 (I53-40A and I53-40B);
(iii) SEQ ID NO:3 and SEQ ID NO:24 (I53-40A and I53-40B.1);
(iv) SEQ ID NO:23 and SEQ ID NO:4 (I53-40A.1 and I53-40B);
(v) SEQ ID NO:35 and SEQ ID NO:36 (I53-40A genus and I53-40B genus);
(vi) SEQ ID NO:5 and SEQ ID NO:6 (I53-47A and I53-47B);
(vii) SEQ ID NO:5 and SEQ ID NO:27 (I53-47A and I53-47B.1);
(viii) SEQ ID NO:5 and SEQ ID NO:28 (I53-47A and I53-47B.1NegT2);
(ix) SEQ ID NO:25 and SEQ ID NO:6 (I53-47A.1 and I53-47B);
(x) SEQ ID NO:25 and SEQ ID NO:27 (I53-47A.1 and I53-47B.1);
(xi) SEQ ID NO:25 and SEQ ID NO:28 (I53-47A.1 and I53-47B.1NegT2);
(xii) SEQ ID NO:26 and SEQ ID NO:6 (I53-47A.1NegT2 and I53-47B);
(xiii) SEQ ID NO:26 and SEQ ID NO:27 (I53-47A.1NegT2 and I53-47B.1);
(xiv) SEQ ID NO:26 and SEQ ID NO:28 (I53-47A.1NegT2 and 153-47B.1NegT2);
(xv) SEQ ID NO:37 and SEQ ID NO:38 (I53-47A genus and I53-47B genus);
(xvi) SEQ ID NO:7 and SEQ ID NO:8 (I53-50A and I53-50B);
(xvii) SEQ ID NO:7 and SEQ ID NO:32 (I53-50A and I53-50B.1);
(xix) SEQ ID NO:7 and SEQ ID NO:33 (I53-50A and I53-50B.1NegT2);
(xx) SEQ ID NO:7 and SEQ ID NO:34 (I53-50A and I53-50B.4PosT1);
(xxi) SEQ ID NO:29 and SEQ ID NO:8 (I53-50A.1 and I53-50B);
(xxii) SEQ ID NO:29 and SEQ ID NO:32 (I53-50A.1 and I53-50B.1);
(xxiii) SEQ ID NO:29 and SEQ ID NO:33 (I53-50A.1 and I53-50B.1NegT2);
(xxiv) SEQ ID NO:29 and SEQ ID NO:34 (I53-50A.1 and I53-50B.4PosT1);
(xxv) SEQ ID NO:30 and SEQ ID NO:8 (I53-50A.1NegT2 and I53-50B);
(xxvi) SEQ ID NO:30 and SEQ ID NO:32 (I53-50A.1NegT2 and I53-50B.1);
(xxvii) SEQ ID NO:30 and SEQ ID NO:33 (I53-50A.1NegT2 and 153-50B.1NegT2);
(xxviii) SEQ ID NO:30 and SEQ ID NO:34 (I53-50A.1NegT2 and 153-50B.4PosT1);
(xxix) SEQ ID NO:31 and SEQ ID NO:8 (I53-50A.1PosT1 and I53-50B);
(xxx) SEQ ID NO:31 and SEQ ID NO:32 (I53-50A.1PosT1 and I53-50B.1);
(xxxi) SEQ ID NO:31 and SEQ ID NO:33 (I53-50A.1PosT1 and 153-50B.1NegT2);
(xxxii) SEQ ID NO:31 and SEQ ID NO:34 (I53-50A.1PosT1 and 153-50B.4PosT1);
(xxxiii) SEQ ID NO:39 and SEQ ID NO:40 (I53-50A genus and I53-50B genus);
(xxxiv) SEQ ID NO:9 and SEQ ID NO:10 (I53-51A and I53-51B);
(xxxv) SEQ ID NO:11 and SEQ ID NO:12 (I52-03A and I52-03B);
(xxxvi) SEQ ID NO:13 and SEQ ID NO:14 (I52-32A and I52-32B);
(xxxv) SEQ ID NO:15 and SEQ ID NO:16 (I52-33A and I52-33B) (xxxvi) SEQ ID NO:17 and SEQ ID NO:18 (I32-06A and I32-06B);
(xxxvii) SEQ ID NO:19 and SEQ ID NO:20 (I32-19A and I32-19B);
(xxxviii) SEQ ID NO:21 and SEQ ID NO:22 (I32-28A and I32-28B); and
(xxxix) SEQ ID NO:23 and SEQ ID NO:24 (I53-40A.1 and I53-40B.1).
4. The nanostructure of claim 1 , wherein the number of identical first polypeptides in each first assembly is different than the number of identical second polypeptides in each second assembly.
5. The nanostructure of claim 4 , wherein each first assembly comprises five copies of the identical first polypeptide, and each second assembly comprises three copies of the identical second polypeptide.
6. The nanostructure of claim 5 , wherein the nanostructure comprises twelve first assemblies and twenty second assemblies.
7. The nanostructure of claim 4 , wherein each first assembly comprises three copies of the identical first polypeptide, and each second assembly comprises two copies of the identical second polypeptide.
8. The nanostructure of claim 7 , wherein the nanostructure comprises twenty first assemblies and thirty second assemblies.
9. The nanostructure of claim 4 , wherein each first assembly comprises five copies of the identical first polypeptide, and each second assembly comprises two copies of the identical second polypeptide.
10. The nanostructure of claim 9 , wherein the nanostructure comprises twelve first assemblies and thirty second assemblies.
11. The nanostructure of claim 1 , further comprising a cargo within the nanostructure.
12. The nanostructure of claim 11 , wherein the cargo comprises a nucleic acid.
13. A nanostructure, comprising:
(a) a plurality of first assemblies, each first assembly comprising a plurality of identical first polypeptides, wherein the first polypeptides comprise an amino acid sequence at least 95% identical to the amino acid sequences SEQ ID NO:7; and
(b) a plurality of second assemblies, each second assembly comprising a plurality of identical second polypeptides, wherein the second polypeptides comprise an amino acid sequence at least 95% identical to the amino acid sequence SEQ ID NO:34;
wherein the plurality of first assemblies non-covalently interact with the plurality of second assemblies to form a nanostructure.
14. A nanostructure, comprising:
(a) a plurality of first assemblies, each first assembly comprising a plurality of identical first polypeptides, wherein the first polypeptides comprise the amino acid sequence of SEQ ID NO:7; and
(b) a plurality of second assemblies, each second assembly comprising a plurality of identical second polypeptides, wherein the second polypeptides comprise the amino acid sequence of SEQ ID NO:34;
wherein the plurality of first assemblies non-covalently interact with the plurality of second assemblies to form a nanostructure.