Channelrhodopsins for optical control of cells
The invention, in some aspects relates to compositions and methods for altering cell activity and function and the introduction and use of light-activated ion channels.
1. A recombinant nucleic acid vector comprising a heterologous nucleic acid molecule insert encoding a light-activated ion channel amino acid polypeptide sequence, the amino acid sequence having at least 90% amino acid identity to amino acids 61-295 as set forth in SEQ ID NO: 7 and at least 95% identity to the amino acids 296-325 as set forth as SEQ ID NO: 7.
2. The recombinant nucleic acid vector of claim 1 , wherein the amino acid sequence has at least 95% amino acid identity to amino acids 61-295 as set forth in SEQ ID NO: 7 and at least 95% identity to the amino acids 296-325 as set forth as SEQ ID NO: 7.
3. The recombinant nucleic acid vector of claim 1 , wherein the amino acid sequence has at least 99% amino acid identity to amino acids 61-295 as set forth in SEQ ID NO: 7 and at least 95% identity to the amino acids 296-325 as set forth as SEQ ID NO: 7.
4. The recombinant nucleic acid vector of claim 1 , wherein the amino acid sequence has at least 99% amino acid identity to amino acids 61-295 as set forth in SEQ ID NO: 7 and at least 99% identity to the amino acids 296-325 as set forth as SEQ ID NO: 7.
5. The recombinant nucleic acid vector of claim 1 , wherein the amino acid sequence has at least 99% amino acid identity to amino acids 61-295 as set forth in SEQ ID NO: 7 and at least 99% identity to the amino acids 296-325 as set forth as SEQ ID NO: 7.
6. The recombinant nucleic acid vector of claim 1 , wherein the amino acid sequence is as set forth in SEQ ID NO: 7.
7. The recombinant nucleic acid vector of claim 1 , wherein the nucleic acid molecule insert is as set forth in SEQ ID NO: 8.
8. The recombinant nucleic acid vector of claim 1 , wherein the vector further comprises one or more additional nucleic acid sequences.
9. The recombinant nucleic acid vector of claim 1 , wherein the light-activated ion channel amino acid polypeptide sequence further comprises a heterologous fusion protein sequence.
10. The recombinant nucleic acid vector of claim 1 , wherein the sequence of the nucleic acid molecule insert is optimized for expression in a human host cell.
11. The recombinant nucleic acid vector of claim 1 , wherein the vector is an expression vector.
12. An isolated host cell comprising the vector of claim 1 , wherein the host cell is a mammalian cell.
13. The host cell of claim 12 , wherein the cell is an excitable cell.