System for hypothermic transport of samples
A system for the hypothermic transport of biological samples, such as tissues, organs, or body fluids. The system includes a self-purging preservation apparatus to suspend a sample in preservation fluid and perfuse a tissue with preservation fluid. The self-purging preservation apparatus is placed in an insulated transport container having a cooling medium. When assembled, the system allows for transport of biological samples for extended periods of time at a stable temperature.
1. A canister for transporting a biological sample, comprising:
a sample storage chamber comprising a floor and walls and configured to receive a biological sample;
a removable lid operable to couple with the walls to form a sterile environment within the sample storage chamber, the removable lid having a chamber, a fill port, a vent between the chamber and an exterior of the canister, and a valve between the chamber and the sample storage chamber, the removable lid further comprising a bottom portion that forms an upper portion of the sample storage chamber when coupled, wherein the valve and vent are positioned to direct fluids in the sample chamber to the valve, thereby allowing gases to be purged from the sample chamber through the valve to the chamber and from the chamber through the vent to the exterior of the canister as the sample chamber is filled with fluid through the fill port;
an adapter, separate from the fill port, and configured to couple to and suspend the biological sample from the bottom portion within the sterile environment without the biological sample contacting the floor and the walls;
a temperature sensor within the sterile environment and in communication with a temperature display readable outside the sterile environment without opening the removable lid; and
a cooling media for maintaining a stable temperature within the sterile environment.
2. The canister of claim 1 , further comprising a source of oxygen-containing gas in fluid communication with the sample storage chamber.
3. The canister of claim 2 , wherein the source of an oxygen-containing gas is a compressed gas cylinder.
4. The canister of claim 1 , wherein the cooling media comprises eutectic cooling blocks.
5. The canister of claim 1 , wherein the stable temperature is between 2-8° C.
6. The canister of claim 1 , wherein the biological sample comprises tissues or organs.
7. The canister of claim 1 , wherein the biological sample is a container holding body fluids.
8. The canister of claim 1 , further comprising a pressure sensor, or an oxygen sensor.