Collagen scaffolds
View Patent ↗Methods for preparing and using collagen extracts and collagen scaffolds are provided. Additionally methods and related kits for the repair of articular tissue using the collagen material are provided.
1. A collagen implant configured to repair tissue exposed to synovial fluid, the collagen implant comprising:
an implant body having a size and shape that is configured for implantation into an articular joint where synovial fluid is present, the implant body entirely comprised of a self-assembly of enzyme-solubilized collagen fibers comprising tropocollagen or atelocollagen, the collagen fibers having resistance to degradation by synovial fluid; and
wherein the collagen implant is a compressible and expandable material that is up to about 22 mm in diameter in an non-expanded state and has a collagen concentration of at least 10 mg/mL.
2. The collagen implant of claim 1 , wherein the collagen implant is expandable.
3. The collagen implant of claim 1 , further comprising calcium.
4. The collagen implant of claim 1 , wherein the collagen fibers comprise calcium.
5. The collagen implant of claim 1 , wherein the collagen implant is a sponge.
6. The collagen implant of claim 1 , wherein the collagen implant is capable of absorbing at least 3 ml of blood.
7. The collagen implant of claim 1 , wherein the collagen implant is capable of absorbing at least 9 ml of blood.
8. The collagen implant of claim 1 , wherein the collagen implant is cylindrical.
9. The collagen implant of claim 8 , wherein the collagen implant is about 22 mm in diameter.
10. The collagen implant of claim 8 , wherein the collagen implant is about 30 mm in length.
11. The collagen implant of claim 1 , wherein the collagen implant comprises collagen obtained from a bovine knee.
12. The collagen implant of claim 6 , comprising less than about 25 ug/g of nucleic acids per gram of collagen implant.
13. The collagen implant of claim 6 , comprising less than about 50,000 ng/g of DNA per gram of collagen implant.
14. The collagen implant of claim 6 , comprising less than about 5,000 uM/g of phospholipids per gram of collagen implant.
15. The collagen implant of claim 6 , comprising less than about 2,000 ug/g of GAG per gram of collagen implant.
16. The collagen implant of claim 1 , further comprising a therapeutic protein.
17. The collagen implant of claim 1 , comprising less than about 25 ug/g of nucleic acids per gram of collagen implant, less than about 50,000 ng/g of DNA per gram of collagen implant, less than about 5,000 uM/g of phospholipids per gram of collagen implant, and less than about 2,000 ug/g of GAG per gram of collagen implant, wherein the collagen implant is capable of absorbing at least 3 ml of blood.
18. The collagen implant of claim 1 , comprising less than about 25 ug/g of nucleic acids per gram of collagen implant, less than about 50,000 ng/g of DNA per gram of collagen implant, and less than about 5,000 uM/g of phospholipids per gram of collagen implant, wherein the collagen implant is capable of absorbing at least 3 ml of blood.
19. The collagen implant of claim 1 , comprising less than about 25 ug/g of nucleic acids per gram of collagen implant, less than about 50,000 ng/g of DNA per gram of collagen implant, and less than about 2,000 ug/g of GAG per gram of collagen implant, wherein the collagen implant is capable of absorbing at least 3 ml of blood.
20. A collagen implant configured to repair a ruptured ligament, the collagen implant comprising:
an implant body having a size and shape that is configured for implantation near the ruptured ligament, the implant body entirely comprised of a self-assembly of collagen fibers comprising tropocollagen or atelocollagen formed by heating a neutralized collagen slurry; wherein the neutralized collagen slurry has a collagen concentration of greater than 10 mg/ml; and
wherein the collagen implant is a compressible material and the collagen fibers have a resistance to degradation by synovial fluid.
21. The collagen implant of claim 20 , wherein the collagen fibers comprise calcium.
22. The collagen implant of claim 20 , wherein the collagen slurry comprises calcium.
23. The collagen implant of claim 20 , wherein the collagen implant is a sponge.
24. The collagen implant of claim 20 , wherein the collagen implant is cylindrical.
25. The collagen implant of claim 20 , further comprising a therapeutic protein.
26. The collagen implant of claim 20 , comprising less than about 25 ug/g of nucleic acids per gram of collagen implant, less than about 50,000 ng/g of DNA per gram of collagen implant, less than about 5,000 uM/g of phospholipids per gram of collagen implant, and less than about 2,000 ug/g of GAG per gram of collagen implant, wherein the collagen implant is capable of absorbing at least 3 ml of blood.
27. The collagen implant of claim 25 , wherein the one or more therapeutic proteins include one or more hormones, cytokines, growth factors, clotting factors, anti-protease proteins, angiogenic proteins, anti-angiogenic proteins, fibronectin, and bone morphogenic proteins.
28. The collagen implant of claim 1 , wherein the self-assembly of collagen fibers are not crosslinked.
29. The collagen implant of claim 20 , wherein the self-assembly of collagen fibers are not crosslinked.