Protein arrays and methods of using and making the same
Methods and devices are provided for preparing a protein array having a plurality of proteins. In one embodiment, the method includes providing a plurality of nucleic acids each having a predefined sequence and expressing in vitro a plurality of proteins from the plurality of nucleic acids. In another embodiment, protein arrays having a solid surface and a microvolume are also provided. The solid surface can have a plurality of anchor oligonucleotides capable of hybridizing with a plurality of nucleic acids. The microvolume can cover each of the plurality of anchor oligonucleotides and can be configured to produce a polypeptide from each of the plurality of nucleic acids.
1. A method for preparing a plurality of proteins comprising:
(a) providing a plate comprising a plurality of wells,
wherein the plurality of wells comprises a plurality of nucleic acids, each nucleic acid having a predefined sequence and encoding a protein, and
wherein the nucleic acids of each well are immobilized on one or more supports; and
(b) expressing in vitro a plurality of proteins from the plurality of nucleic acids,
wherein the proteins are expressed in the wells of the plate in volumes having a size of 0.5 picoliters to 100 nanoliters.
2. The method of claim 1 , wherein the plurality of nucleic acids is immobilized on beads.
3. The method of claim 1 , wherein the nucleic acids of each well are immobilized on one bead.
4. The method of claim 1 , wherein the plurality of nucleic acids is immobilized on the surface of the plate.
5. The method of claim 1 , wherein each well in the plurality of wells comprises a nucleic acid having a unique predetermined sequence.
6. The method of claim 5 , wherein the plurality of proteins comprises a plurality of different proteins.
7. The method of claim 1 , wherein the plurality of nucleic acids comprises nucleic acids having different predetermined sequences.
8. The method of claim 7 , wherein the nucleic acids of each well comprise the same predetermined sequence.
9. The method of claim 7 , wherein the plurality of proteins comprises a plurality of different proteins.
10. The method of claim 1 , wherein the proteins are expressed in the wells of the plate in volumes having a size of 1 picoliter to 1 nanoliter.