Compositions and methods comprising histidyl-tRNA synthetase splice variants having non-canonical biological activities
Isolated histidyl-tRNA synthetase splice variant polynucleotides and polypeptides having non-canonical biological activities are provided, as well as compositions and methods related thereto.
1. An intranasal spray, or an inhalation or aerosol delivery vehicle, comprising a physiologically acceptable carrier and a fusion polypeptide, wherein the fusion polypeptide comprises a histidyl-tRNA synthetase (HRS) polypeptide fused to an Fc fragment, wherein the HRS polypeptide consists of a sequence that has at least 95% identity along its length to SEQ ID NO: 6, comprises a WHEP domain, and lacks a functional aminoacylation domain.
2. The intranasal spray, or an inhalation or aerosol delivery vehicle, of claim 1 , wherein the HRS polypeptide consists of a sequence that has at least 98% identity along its length to SEQ ID NO: 6.
3. The intranasal spray, or an inhalation or aerosol delivery vehicle, of claim 1 , wherein the N-terminal region of the HRS reference polypeptide is truncated by 1, 2, or 3 amino acids.
4. A method of delivering a fusion polypeptide composition directly to lungs, comprising administering to a subject the intranasal spray, or inhalation or aerosol delivery vehicle, of any one of claims 1 - 3 .