IP Library › Granted Patent US 12,221,720
Granted Patent B2
US 12,221,720 · App. 16/763,681 · Granted Feb 11, 2025

Methods for determining spatial and temporal gene expression dynamics during adult neurogenesis in single cells

Inventors: Naomi Habib (Cambridge, MA); Aviv Regev (Cambridge, MA); Eugene Drokhlyansky (Cambridge, MA); Anindita Basu (Cambridge, MA); Inbal Avraham-Davidi (Cambridge, MA); Orit Rozenblatt-Rosen (Cambridge, MA); David A. Weitz (Cambridge, MA)
Assignees: The Broad Institute, Inc.; Massachusetts Institute of Technology; President and Fellows of Harvard College
C40B30/06A61K35/30C12N5/0619C12N5/0623C12Q1/6806C12Q1/6881C12N5/0081C12Q2600/158
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Quick Facts
Patent No.
US 12,221,720
App. No.
16/763,681
Granted
Feb 11, 2025
Kind
B2
Abstract

Techniques Nuc-seq, Div-Seq, and Dronc-Seq are allow for unbiased analysis of any complex tissue. Nuc-Seq, a scalable single nucleus RNA-Seq method, can sensitively identify closely related cell types, including within the adult hippocampus. Div-seq combines Nuc-Seq with EdU-mediated labeling of proliferating cells, allowing tracking of transcriptional dynamics of newborn neurons in an adult neurogenic region in the hippocampus. Dronc-Seq uses a microfluidic device to co-encapsulate individual nuclei in reverse emulsion aqueous droplets in an oil medium together with one uniquely barcoded mRNA-capture bead.

Claims (24)

1. A method of producing a temporally phased single-cell sequencing library comprising cells along a continuous trajectory of adult neurogenesis in the adult brain, the adult dorsal ganglion (DG) or in the adult spinal cord, said method comprising:

(a) treating more than one population of neurogenic cells of heterogeneous cell types or subtypes, with a nucleoside analogue, wherein the neurogenic cell is selected from the group consisting of: a neuronal stem cell, a neuronal precursor cell, a neuroblast, an immature neuron and a newborn neuron, and wherein the nucleoside analogue is incorporated into replicating DNA and is configured for labeling with a detectable marker;

(b) isolating a first population of neurogenic cells at one time point and isolating at least one other population of neurogenic cells at a later time point;

(c) staining the nucleoside analogue incorporated into replicating DNA with the detectable marker within each population of neurogenic cells, wherein the replicating DNA is stained with the detectable marker;

(d) sorting the stained and/or unstained neurogenic cells; and

(e) sequencing the RNA from the sorted single neurogenic cells, whereby single cell gene expression data is obtained for neurogenic cells at different stages of neurogenesis.

2. A method of determining an expression profile for a neurogenic cell along a continuous trajectory of adult neurogenesis in the adult brain, the adult dorsal ganglion (DG) or in the adult spinal cord, said method comprising:

(a) treating more than one population of neurogenic cells of heterogeneous cell types or subtypes, with a nucleoside analogue, wherein the neurogenic cell is selected from the group consisting of: a neuronal stem cell, a neuronal precursor cell, a neuroblast, an immature neuron and a newborn neuron, and wherein the nucleoside analogue is incorporated into replicating DNA and is configured for labeling with a detectable marker;

(b) isolating a first population of neurogenic cells at one time point and isolating at least one other population of neurogenic cells at a later time point;

(c) staining the nucleoside analogue incorporated into the replicating DNA with the detectable marker within each population of neurogenic cells, wherein the replicating DNA is stained with the detectable marker;

(d) sorting the stained and/or unstained neurogenic cells;

(e) sequencing the RNA from the sorted single neurogenic cells, whereby single cell gene expression data is obtained for neurogenic lineage cells at different stages of maturation; and

(f) determining an expression profile for each identified cell or cell sub-type based on the gene expression data.

3. The method of claim 2 , wherein the expression profile identifies the cell as an adult newborn neuron or immature neuron of the spinal cord and comprises: Gad1, Gad2, Pbx3, Meis2 and Runx1t1.

4. The method of claim 2 , wherein the expression profile identifies the cell as a neuronal stem cell, a neuronal precursor cell, a neuroblast, an immature neuron or a newborn neuron and comprises at least one of: Fabp7, Asc1, Insm1, Notch1, Eomes, Tgfb2, Chd7, Sox5, Sox4, Neurod1, Neurod2, Sema3c, Igfbp11, Sox11, Slc6a1, Dcx, Grin2b, Gad1, Bhlhe22, Sox8, Sox10, Dip2a, Ncoa3, Rorb, |d3, Sox9, Mndal, Ifi203, Flna, Zeb1, Sox9/2, Fezf2, Pax3, |d3/4, Cdk2, Chd5, Hdac7, Sox2, Sox6, Cdk2ap1, Cdk9, Cdk12, Kif11, Kif21b, Kif17, Kdm5c, Kdm7a, Hdac8, Kdm2b, Hdac5, Chd1, Kdm3b, Rrm2, Gpr56, Draxin, Mfap4, Gfap, Mt1, Aldoc, Clu, Aqp4, Mt2, Cst3, Slc1a2, Pbxip1, Fgfr3, Slc2a1, Slpr1, Fxyd1, Glu1, Slc1a3, Olig2, Aldh111, Pre1p, Vim, Pax6, Re1n, Gpr17, Tcf712, Nfib, Dbx2,Emx1, Sox1, Prox1, Dlx1, Foxg1, Slit1, Gad2, Mbp, Meg3, Grm2, St3gal1, C1l3, Kcnc4, Cebpd, C1l2, Trpc6, Pter, Kif26b, Gsg1l, Igfbp5, Fam84a, Lrtm2, Ehd1, Gfra1, Calb1, Doc2b, Lct, Pde1b, Dockl0, Rasll0a, St18, Jun, Dbpht2, Pcdh8, Slc30a3, Mapk3, Sipall2, Gabrd, Sytl7, Stxbp6, Lingo2, Pde7b, Anp32a, Gprin1, Cacng3, Slc39a6, Limd2, Plekha2, Marcks, Rab40b, Npnt, Ppp1r1a, B4galt5, Chst15, Crlf 1, Dsp, Dgkh, Ntng1, Ankrd6, Trp53il1, Gng7, Hlf, Dek, Synpr, Gfod1, Pitpnm2, Ncdn, Gp1bb, Marcksl1, Gpc4, Cplx2, Plk5, Fam163b, Bdnf, Pcp4, Cbfa2t3, Ngef, Rilpl1, Nkain2, Arxes1, Lgi3, Atp2b4, Gfra2, Vav3, Lzts3, Fam3c, Zfpm2, Amer3, 6530402F18Rik, Rragd, Pkig, Acvr1c, Foxo1, Napep1d, Cecr2, Pde8b, Cedc85a, Rps23, Dlg3, Fnip2, Ptchd1, Prkd1, Smad3, Ncam2, Faml9a2, Dgat2, Ptbp3, Htr4, Slitrk2, Thsd7a, Clgtnf4, Lhfpl2, Tmem198, B3galt5, Ncald, Rp132, Rmnd5a, Cdh13, Cyp7bl, Rplpl, Sh3bgrl3, Foxo3, Diap2,Rps15, Sec1412, Spsb1, Btgl, Kcnip4, Nov, Pex51, Ldb2, Cadps2, Fibed1, Fxyd6, Hs6st3, Kcnk9, Cnih3, Lypd1, Mal2, Ramp1, Ankrd33b, A830036E02Rik, Amigo2, Acan, T112, Dock1 l, Krt12, Kcnk2, Ptpn5, Kalrn, Ntsr2, A630077J23Rik, Fat3, Rgsl4, Cacng5, 9130019P16Rik, Gprl2, Itga7, Igfbp4, Kcnv1, Car7, Gcnt4, 5430416009Rik, Serpinala, Drd1a, Map3k15, Zdhhc23, Zfp46, Drap1,Aphlb, ENSMUSG00000095041,Mehrl, Ntf3, Cel21a, Atxn7llos1,Npas4, Myolb, Tac1, Arhgap6, 4930578G10Rik, Lingo3, Sorcs2, Srm, Rara, Tmem154, Igdcc3, Tpsg1, Arap2, Hunk, Slco5a1, 1700024P16Rik, St8sia6, BC046251, Gml1417, 2610042L04Rik, Grip1os3, Kitl, Palm2, Ptplb, Blnk, Abcc4, Fgf5, Arhgef3, Nptx2, Cdh7, Asic2, Lrp12, Cd3d, Adcy 1, Kcnj4, Tiam2, Prdm8, F730043M19Rik, Lphn2, Bell1b, Sponl, Adamts9, Grik4, Car4, Cdh24, Gm20751, Cpne9, Shisa6, Slc22a4, Galnt3, Slco2a1, Efr3b, Homer3, Lnx1, Parp8, 3110047P20Rik, Bok, Tspan17, Eve, Socs2, Gm12296, Tbata, Nkd2, Mapkll, Rerg, 1700017B05Rik, Kent2, Scubel, Asl, Dpf3, Pvrl3, Clmp, 8430431K14Rik, Adamts14, Ptgs2, Traf3, P2rx7, Stac2, Rhebll, Nkain3, Hesl, Ankrd29, Smarcd3, Smoc2, Robo3, Fstl4, Cacnali, Slit2, Ak4, Tbcld1, Ppp4r4, Cpne7, Gukl, Slc26a4, E330009J07Rik, Sema3e, Zdhhe14, Nell1, Lingol, Cpne6, Wasf1, Lmo4, Unc5a, Zfp831, Ywhah, 4930447C04Rik, Cabp7, 1116, Trhde, Kif1c, Arhgef26, Tdrd5, 4921534H16Rik, Cables2, Cpne4, Rasal1, Rprm1, Sstr4, Rltpr, Gpr26, Prkab2, Lrtm1, Ryr3, Fam189a1, Khdrbs3, Hs3st4, Raly1, Golga7b, Specc1, Fam5b, Frmd6, Mdga1, Arc, Egr1, Pkp2, Neurod6, Ptpru, Mgat4c, Robo1, Ankrd27, Cnr1, Thsd4, Raver2, Myadm, Aff3, Cnnm4, Nr4a3, Slc9a1, Gnptab, 3110039M20Rik, Esyt2, Mrpl3, Rab26, Sv2b, Gml1201, Dkk3, Stat2, Pde1a, Cedc88c, Stom11, Ttc8, Actr3b, Map3k7, Gdpdl, Cep78, AI115009, Pip4k2c, Asap2, Msra, Pfkfb3, Kit, Dlx6os1, Grik1, Rbms3, Nxph1, Dlx1as, Dpp10, Grip2, Cntn5, Slc32a1, Coll9a1, Alk, Lhx6, A530058N18Rik, Kenmb2, Kcnip1, Foxred2, Ubash3b, Arx, Zmat4, Gm13629, Sema6c, Galntl6, Pnoc, Sgez, Unc5d, Cntnap4, Grin2d, Tm6sf1, Btbdl1, Arl4c, Pla2g4e, Uspl3, Lrrtm3, Dner, Ankl, Rab3b, Adrala, Gabrg3, Hapln1, Hen3, Myol6, Fgf9, Tox2, Gm15881, Zfp57, Cit, Amy 1, Ptprt, Gpsm1, Usp29, Rcan2, Igsf11, Zcchc12, Sema4g, AI504432, Dlcl, Ece2, E230016M1lRik, Olfm2, Gad1, Tbcld2b, Dpysl5, Spock3, Osbpl3, Impdhl, A230057D06Rik, Erbb4, Coro6, Ptprm, Trim62, Dlx6, Astn2, Plekhh2, Plxdc2, Dchs1, Fam124a, Fmn1, Frmd5, Slc35fl, A330076H08Rik, Rims3, Neb, BC030500, Fam20c, Bend6, Afap1, Nyap2, Hook2, Gria4, Atp8a2, Evl, Sen9a, Rcan3, Dnm3os, Camkl, Hdac9, Slc35e3, Pknox2, Garn13, B930041F14Rik, Ptpn4, Fnbp11, Fchsd2, Inha, Maf, Gm14204, Pam, Eps8, Scrt1, Kcna6, Lin7a, Capn2, Srgap1, Bend4, Grebl1, Pde9a, Hdac6, Cacna2d2, Lama2, Zcchc24, Htra1, Hepacam, Gpr3711, Ttyh1, Ppap2b, Kenj10, Slc6a11, Hif3a, Htr2c, Wdr86, Col9a3, Kcnj13, Ttr, Steap2, Tmem72, Pr1r, Slc4a5, Rbm47, Trpm3, Cab391, Whrn, Enpp2, Car14, Dock6, Lama5, Bad, Ki, Lbp, Eps812, Lrriq1, Psd2, Frem1, Rdh5, Fap, S1c6a20a, Slc2a12, Gmnc, Ppplr1b, F5, Atp2b3, Col4a5, S1c7a10, Mfrp, Carl2, Slc13a4, Ezr, Ccdc108, Sulf 1, Abhd2, Stk39, Ucp2, Dab2, Mitf, S1c6a20b, Col4a3, Prdml6, Otx2, Trpv4, Fras1, Efs, Lrrc23, 1110059M19Rik, Wdr78, Folr1, Ace, Fhad1, Mpp7, Zkscan3, Ccdc135, Vat11, Sh3d19, Spag16, Abca4, Arhgap42, Cpne2, S1c4a10, Slc4a2, Slc12a2, Spata17, Ppfibp2, Phldb2, Calml4, Msx1, Lmx1a, Dnahc6, Myo7a, Neat1, BC021767, Nhs12, Ccdc39, Clic6, Mroh7, Acad8, Col4a4, Ccdc114, Oca2, Dnah11, Stxbp4, Vwa3a, Arsg, Mycbpap, 4932438H23Rik, St6galnac2, Best3, Ifi27, Gm216, 5930403L14Rik, 4632427E13Rik, Cgnl1, Sostdc1, Zfpl85, Lef1, 1500015010Rik, Slcola4, Sned1, Pltp, Arl6ipl, Synm, Fam161a, Igf2, Sytl2, Six3os1, Nwd1, Slc16a8, Ifi2711, Pcolce2, Ccdc141, Kcne2, Dnahc2, Slc38a3, L3mbt13, Cldn2, Aar2, Dnahc7b, Baiap2l1, Gm26648, Acacb, Gt(ROSA)26Sor, Sgk1, Ttc21a, Tuft1, Otx2os1, Rfx2, Efcab1, Frrs1, Crb2, Epn3, Mospd1, Acaa2, Ctnnall, Sema3b, Tmem237, Elov17, Pcp411, Esrrb, Mapk4, Zfp280d, Cfap44, Wdr52, Gadd45g, Cldn1, Sgms1, Msx1as, Spag6, Lhb, Rilp, Ccdc24, Ifi35, 6720401G13Rik, Amer1, Zfp119a, Crhr2, Gm17200, Csrp2, Dmpk, Gm7173,Cnst, Ctnna1, Lepr, Intu, Antxr1, Atp10d, E530011L22Rik, Slc39a13, Ccnd3, Spata13, Igfbp2, Top3b, Ephx1, Cish, Vcam1, Tns1, Setd5, Tubb4b, 2010015L04Rik, Igfn1, Krt18, Slco1c1, Myo5c, Apls2, Arhgap28, Frmd4b, Efcab12, Dhrsl1, Tjp3, Spef2, Tinagl1, Tt151, Sgms2, Cf1ar, Stard13, |d1, 4930556M19Rik, Ssfa2, Iqub, Slc5a3, Plekhg3, Klhdc7a, Flcn, Fam86, Bcl211, Spag8, Parp4, Arid5b, Tc2n, Suv420h2, Kif9, Gpr98, Clqtnf5, Foxj1, Zic5, Sgk3, Cob111, Phactr2, Sorbs3, Steap1, Hya11, Armc2, Spint2, Aox1, Serh1, Tsix, Tekt1, 1810022K09Rik, C030017K20Rik, Slc12a4, Cfap69, Rail4, Slc3lal, Nek5, Dnah2, Nqol, Col8a1, Tes, Scarf1, Pcbp3, Cd59a, Ccp110, Syne2, Pid1, 9530091C08Rik,Ptgds, D630024D03Rik, Prob 1, Histlh2br, Histlh2bq, Slc16a6, Slc16a2, Slc23a2, Strip2, Fam53b, Esrrg, Heg1, Pikfyve, Lamb2, Sowahc, Pex11a, Gm13139, Trip10, Slc19a1, Brwd3, Mast4, Mapk6, Tspan32os, Cgn, Tbcld9, Cpq, Heatr8, Msi1, Agtrap, Rfwd3, 9830147E19Rik, Shroom3, Slc39a12, Msi2, Col27a1, Mok, Rbbp8, Nckap5, 4930522L14Rik, Cspg4, Tnr, Pcdh15, Matn4, Vcan, Pdgfra, Dscam, Olig1, Hip1r, Ppfibp1, Grin3a, Neu4, Itga9, 3110035E14Rik, Slc1a1, Cacng4, Pllp, Cercam, Rgcc, Traf4, Ugdh, Elfn2, Cask, Cob1, Xylt1, Clq1, Cyth4, Hmha1, Inpp5d, Laptm5, Cd33, Csflr, Mpeg1, Abca9, Pag1, Cldn1 l, Mobp, Mag, Mal, Mog, Qdpr, Csrp1, Efnb3, Ttyh2, Epb4.113, Apod, Slc44a1, Kndc1, Gpr37, Tmeml51a, Cntn2, Itpk1, Car2, Cryab, Tspan2, Tnfaip6, Gm10471, Plekhh1, Gm7347, Hapln2, Arhgap23, Myrf, Arhgefl0, Ugt8a, Dip2a, Asphd1, 5031410I06Rik, Fa2h, Ndrgl, Stmn4, Jam3, Unc5b, Taldo1, Erbb2ip, Tmem63a, Ype12, Wdr54, Tprn, Gm21847, Ado, Kctdl3, Serinc5, Speer7-psl, Adamts4, Tppp3, Gm10220, Pik3c2b, Speer4a, Rcbtbl,Desil, Slain1, Pacs2, Pigz, Opalin, Grb14, Endodl, Scd1, Smad7, Litaf, Pip4k2a, Rtkn, Nkain1, Agpat4, Dusp26, Gjb1, Kctd3, Arrdc3, Dnajb2, Speer8-ps1, Evi2a, Speer4d, Slc6a9, Hid1, Rhob, Galnt6, Gjc2, 1133, Gnail, Mcam, 1700047M11Rik, Plin4, Speer4b, Lpar1, Arrdc2, 5031439G07Rik, Dixdcl, Ephbl, Ubxn1, Pgm21l, Cpm, Chn2, Gpt, Usp30, 4933400F21Rik, Trim59, Bcas1, Pak7, Adipor2, Gstm7, Inpp5j, Micall1, Sec1415, Tm7sf3, Spock1, Tyro3, Nrsn1, Plc11, Ankrdl3a, Pls1, Ablim2, Thumpd1, Mpp2, Slpr1, Gja1, Sdc4, Farp1, Mlc1, Pla2g7, Adrbk2, Fam107a, Gjb6, Mfge8, Cyp2d22, Sox9, Prex2, Pnpla7, Vcl, Cbs, Ednrb, C4b, Scara3, Mertk, Lcat, Prodh, Igdcc4, Tril, Fam20a, Acsbg1, Atp13a4, Paqr8,F3, Cldn10, Entpd2, Fam213a, Pdgfrb, Slc15a2, Fgd6, Grin2c, Cxc114, Acss1, Tmem47, Gm13872, Rmst, Gpc5, Grm3, Agt, Cyp2j9, Phka1, Lrig1, Thrsp, Cyp4f 15, Phkg1, Gramd3, Lgi4, Sema4b, Dio2, Acot11, Tenm3, Utp14b, Aifm3, Aldh111, Acat3, Bmpr1b, Slc1a4, Arhgefl9, Myol0, Daam2, Gpam, Etnppl, Gli2, Paqr6, Lfng, 4930480K15Rik, Op1ah, Spry2, Paqr7, Chst2, A1dh7a1, Rfx4, Csgalnact1, 1118, Slc13a3, Drp2, Cm13, Mras, Pcdh10, Klfl5, Anks1, Zic2, Ctso, Rgma, Rftn2, Asrgl1, Fndc4, Snta1, Ppargc1a, Slc41a1, Ax1, 2610203C20Rik, Atl2, Glis3, Nt5c2, Rnf31, Npas3, Gnal3, 2310022B05Rik, Utrn, Abhd3, Fbxo2, Hivep1, Stkl6, Dclk2, Lgr4, Kankl, B3galt2, Zfp219, Dbp, Ap3 ml, Coil a2, Trp53bp2, Fbxo44, Kcnt1, Tmem164, Vegfa, Tead1, Aldh2, Tmem44, Mro, Acadv1, Tspan9, Eya1, Rb1, Lix1, Prrx1, Gm16223, 2010300C02Rik, Airn, Lrrtm4, Gm10364, and Gm10662.

5. The method according to claim 1 , wherein (b) further comprises isolating single nuclei from each of the isolated populations of neurogenic cells.

6. The method according to claim 2 , wherein (b) further comprises isolating single nuclei from each of the isolated populations of cells.

7. The method according to claim 1 , wherein neurogenesis occurs in the dorsal ganglion (DG) or in the adult spinal cord.

8. The method according to claim 1 , wherein neurogenesis occurs in the dentate gyrus of the hippocampus.

9. The method according to claim 1 , wherein neurogenesis occurs in the subventricular zone of the striatum of the adult brain.

10. The method according to claim 2 , wherein neurogenesis occurs in the adult brain.

11. The method according to claim 2 , wherein neurogenesis occurs in the dorsal ganglion (DG) or in the adult spinal cord.

12. The method according to claim 2 , wherein neurogenesis occurs in the dentate gyrus of the hippocampus.

13. The method according to claim 2 , wherein neurogenesis occurs in the subventricular zone of the striatum of the adult brain.

Assignments (6)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 20, 2020
From: AVRAHAM-DAVIDI, INBAL
To: THE BROAD INSTITUTE, INC.
Reel/Frame 053253/0355 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 20, 2020
From: DROKHLYANSKY, EUGENE
To: THE BROAD INSTITUTE, INC.
Reel/Frame 053253/0716 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 20, 2020
From: REGEV, AVIV
To: THE BROAD INSTITUTE, INC.; MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Reel/Frame 053253/0923 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 20, 2020
From: ROZENBLATT-ROSEN, ORIT
To: THE BROAD INSTITUTE, INC.
Reel/Frame 053254/0141 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 20, 2020
From: WEITZ, DAVID A.
To: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Reel/Frame 053254/0240 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 20, 2020
From: BASU, ANINDITA
To: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Reel/Frame 053255/0826 →
Continuity (4)
Provisional Application 62734988 · Sep 21, 2018
Provisional Application 62723425 · Aug 27, 2018
Provisional Application 62585529 · Nov 13, 2017
Related Publication 20210395821A1 · Dec 23, 2021
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