Trialkyl cationic lipids and methods of use thereof
The present invention provides compositions and methods for the delivery of therapeutic agents to cells. In particular, these include novel, trialkyl, cationic lipids and nucleic acid-lipid particles that provide efficient encapsulation of nucleic acids and efficient delivery of the encapsulated nucleic acid to cells in vivo. The compositions of the present invention are highly potent, thereby allowing effective knock-down of a specific target protein at relatively low doses.
1. A lipid particle comprising a lipid selected from the group consisting of:
2. The lipid, particle of claim 1 , wherein the lipid particle further comprises a therapeutic agent, wherein the therapeutic agent is a nucleic add therapeutic agent.
3. The lipid particle of claim 2 , wherein the particle further comprises a non-cationic lipid.
4. The lipid particle of claim 2 , wherein the particle further comprises a conjugated lipid that inhibits aggregation of particles.
5. The lipid particle of claim 2 , wherein the therapeutic agent is siRNA or mRNA.
6. The lipid particle of claim 5 , wherein the therapeutic agent is siRNA.
7. The lipid particle of claim 5 , wherein the therapeutic agent is mRNA.
8. The lipid particle of claim 1 , wherein the lipid particle comprises the lipid Compound 13.
9. The lipid particle of claim 8 , wherein the lipid particle further comprises mRNA as a therapeutic agent.
10. The lipid particle of claim 8 , wherein the lipid particle further comprises siRNA as a therapeutic agent.
11. A pharmaceutical composition comprising a lipid particle of claim 2 and a pharmaceutically acceptable carrier.
12. A method for introducing a therapeutic agent into a cell, the method comprising:
contacting the cell with a lipid particle of claim 2 .
13. A method for the in vivo delivery of a therapeutic agent, the method comprising:
administering to a mammal a lipid particle of claim 2 .
14. A method for treating cancer, a viral infection or a liver disease in a mammal in need thereof, the method comprising:
administering to the mammal a therapeutically effective amount of a lipid particle of claim 2 .