Apparatus and method for extracting pathogens from biological samples
The disclosed embodiments related to an apparatus and methods for biological sample processing enabling isolation and concentration of microbial or pathogenic constituents from the sample. Sample may be obtained directly from a specimen container, such as a vacutainer, and processed directly without risk of user exposure. The disclosed methods and apparatus provide a convenient and inexpensive solution for rapid sample preparation compatible with downstream analysis techniques.
1. An apparatus to capture pathogens from a biological sample, the apparatus comprising:
a valve body ( 120 ) to fluidically couple a sample tube ( 130 ) to each of a syringe body ( 110 , 4 A) and a capture tube ( 135 ), the valve body ( 125 ) having a sample inlet, a first outlet, a second outlet, a channel to connect the sample inlet with each of the first and the second outlets, a first one-way valve ( 210 ) to connect the sample inlet with the channel, a second one-way valve ( 215 ) to connect the channel to the second outlet and a piercing member ( 225 );
a syringe body ( 110 ) having an outer barrel and an inner barrel, the outer barrel further comprising a first reservoir containing a first reagent; and
a plunger piston configured to moveably couple to the outer syringe barrel, the plunger piston comprising an inner syringe barrel, and outer syringe barrel and a gasket between the first and second reservoirs, the inner syringe barrel defining a second reservoir to receive a second reagent.
2. The apparatus of claim 1 , wherein the valve body is configured to receive a sample tube and fluidically connect the sample to the inlet channel.
3. The apparatus of claim 2 , wherein the piercing member is configured to couple through the one-way valve to the sample tube.
4. The apparatus of claim 2 , wherein the valve body further comprises a first and a second sample tube piercing members configured to enter the sample tube, the first sample tube piercing member to fluidically couple to the channel, the second sample tube piercing member to fluidically communicate with the ambient.
5. The apparatus of claim 4 , wherein the second sample tube piercing member further comprises a one-way valve to allow ingress of ambient air into the sample tube.
6. The apparatus of claim 2 , wherein the valve body further comprises a piercing member to pierce the gasket to thereby access the second reagent.
7. The apparatus of claim 6 , wherein the piercing member is configured to enter the second reservoir when the outer syringe containing the first reagent is displaced and the first reservoir is substantially empty.
8. The apparatus of claim 7 , wherein the gasket which separates the first and the second reservoirs is a diaphragm.
9. The apparatus of claim 1 , further comprising a capture tube to removably connect to the valve body, the capture tube having at least one filter membrane configured such that all fluids exiting the valve body pass through the at least one membrane.
10. The apparatus of claim 1 , wherein the first one-way valve opens under a relative negative pressure on the syringe side of the valve and closes under a relative positive pressure on the syringe side of the valve.
11. The apparatus of claim 1 , wherein the second one-way valve opens under a relative positive pressure on the syringe side of the valve, and closes under a relative negative pressure on the syringe side of the valve.
12. The apparatus of claim 1 , wherein the gasket provides automatic sequential delivery of the first and second reagents.