Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d) fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and e) optionally recovery of a fermentation product; wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.
1. A process for preparation of a sugar product from ligno-cellulosic material, comprising:
enzymatic hydrolysis of the ligno-cellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises GH61;
wherein oxygen is added continuously or discontinuously to the ligno-cellulosic material during a first half in time or second half in time of the enzymatic hydrolysis.
2. A process for preparation of a fermentation product from ligno-cellulosic material, comprising:
enzymatic hydrolysis of the ligno-cellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises GH61; and
fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product;
wherein oxygen is added continuously or discontinuously to the ligno-cellulosic material during a first half in time or second half in time of the enzymatic hydrolysis.
3. The process according to claim 1 , wherein the oxygen is added in the form of bubbles.
4. The process according to claim 1 , wherein the enzymatic hydrolysis takes place in a reactor having a volume of 1 m 3 or more.
5. The process according to claim 1 , wherein the total enzymatic hydrolysis time is 5 to 150 hours.
6. The process according to claim 1 , wherein the enzyme composition used retains activity for 30 hours or more.
7. The process according to claim 1 , wherein the enzymatic hydrolysis is conducted at a temperature of 45° C. or more.
8. The process according to claim 1 , wherein the enzyme composition is derived from a fungus, or the enzyme composition comprises a fungal enzyme.
9. The process according to claim 1 , wherein the ligno-cellulosic material has a dry matter content of 10 wt % or more.
10. The process according to claim 1 , wherein the ligno-cellulosic material has a dry matter content of 14 to 33% wt %.
11. The process according to claim 1 , in which the enzymatic hydrolysis takes place in a batch, fed batch and/or continuous culture reactor.
12. The process according to claim 1 , in which oxygen is introduced as air.
13. The process according to claim 8 , wherein the fungus is of the genus Rasamsonia , or the fungal enzyme is a Rasamsonia enzyme.
14. The process of claim 1 , further comprising pre-treatment of the ligno-cellulosic material, prior to the enzymatic hydrolysis.
15. The process of claim 1 , further comprising washing of the ligno-cellulosic material, prior to the enzymatic hydrolysis.
16. The process of claim 1 , further comprising recovery of a sugar product from the enzymatic hydrolysis.
17. The process of claim 1 , further comprising, prior to the enzymatic hydrolysis, pre-treatment of the ligno-cellulosic material and washing of the pre-treated ligno-cellulosic material.
18. The process of claim 1 , further comprising pre-treatment of the ligno-cellulosic material prior to the enzymatic hydrolysis and recovery of a sugar product from the enzymatic hydrolysis.
19. The process of claim 1 , further comprising pre-treatment of the ligno-cellulosic material and washing of the pre-treated ligno-cellulosic material, prior to the enzymatic hydrolysis, and recovery of a sugar product from the enzymatic hydrolysis.
20. The process of claim 2 , wherein the fermentation product is ethanol.