Immunotherapy against neuronal and brain tumors
The present invention relates to peptides, nucleic acids and cells for use in immunotherapeutic methods. In particular, the present invention relates to the immunotherapy of cancer. The present invention furthermore relates to tumor-associated cytotoxic T cell (CTL) peptide epitopes, alone or in combination with other tumor-associated peptides that serve as active pharmaceutical ingredients of vaccine compositions that stimulate anti-tumor immune responses. The present invention relates to 11 novel peptide sequences and their variants derived from HLA class I and class II molecules of human tumor cells that can be used in vaccine compositions for eliciting anti-tumor immune responses.
1. A peptide consisting of the amino acid sequence of AIIDGVESV (SEQ ID NO: 3) in the form of a pharmaceutically acceptable salt.
2. The peptide according to claim 1 , wherein the pharmaceutically acceptable salt is a chloride salt, acetate salt, or trifluoroacetate salt.
3. The peptide of claim 2 , wherein the pharmaceutically acceptable salt is the trifluroacetate salt.
4. The peptide of claim 2 , wherein the pharmaceutically acceptable salt is the chloride salt.
5. The peptide of claim 2 , wherein the pharmaceutically acceptable salt is the acetate salt.
6. The peptide according to claim 1 , wherein the peptide is conjugated with a suitable carrier.
7. The peptide according to claim 6 , wherein the suitable carrier is keyhole limpet haemocyanin or mannan.
8. A pharmaceutical composition comprising the peptide of claim 1 and an immune-enhancing amount of an adjuvant.
9. The pharmaceutical composition of claim 8 , wherein the adjuvant is at least one selected from the group consisting of anti-CD40 antibody, imiquimod, resiquimod, GM-CSF, cyclophosphamide, sunitinib, bevacizumab, interferon-alpha, interferon-beta, CpG oligonucleotides and derivatives, poly-(I:C) and derivatives, RNA, sildenafil, particulate formulations with poly(lactide co-glycolide) (PLG), virosomes, interleukin (IL)-1, IL-2, IL-4, IL-7, IL-12, IL-13, IL-15, IL-21, and IL-23.
10. The pharmaceutical composition according to claim 9 , wherein the adjuvant comprises IL-1.
11. The pharmaceutical composition according to claim 9 , wherein the adjuvant comprises IL-2.
12. The pharmaceutical composition according to claim 9 , wherein the adjuvant comprises IL-4.
13. The pharmaceutical composition according to claim 9 , wherein the adjuvant comprises IL-7.
14. The pharmaceutical composition according to claim 9 , wherein the adjuvant comprises IL-12.
15. The pharmaceutical composition according to claim 9 , wherein the adjuvant comprises IL-13.
16. The pharmaceutical composition according to claim 9 , wherein the adjuvant comprises IL-15.
17. The pharmaceutical composition according to claim 9 , wherein the adjuvant comprises IL-21.
18. The pharmaceutical composition according to claim 9 , wherein the adjuvant comprises IL-23.
19. The peptide in the form of a pharmaceutically acceptable salt of claim 1 , wherein said peptide is produced by solid phase peptide synthesis or produced by a yeast cell or bacterial cell expression system.
20. A composition comprising the peptide of claim 1 , wherein the composition is a pharmaceutical composition and comprises water and a buffer.