Method for making pluripotent stem cells
The present application discloses a method for inducing cells to gain characteristics of naïve stem cell state comprising culturing the cells in the presence of a MUC1* activator.
1. An antibody that specifically binds to Primary Sequence of the MUC1 Growth Factor Receptor (PSMGFR), the antibody comprising light chain complementarity-determining regions (CDR-L1, CDR-L2, and CDR-L3) and heavy chain complementarity-determining regions (CDR-H1, CDR-H2, and CDR-H3), wherein:
the CDR-L1 comprises the amino acid sequence of SEQ ID NO: 20;
the CDR-L2 comprises the amino acid sequence of SEQ ID NO: 21;
the CDR-L3 comprises the amino acid sequence of SEQ ID NO: 22;
the CDR-H1 comprises the amino acid sequence of SEQ ID NO: 23;
the CDR-H2 comprises the amino acid sequence of SEQ ID NO: 24; and
the CDR-H3 comprises the amino acid sequence of SEQ ID NO: 25.
2. The antibody according to claim 1 , wherein the antibody comprises a kappa chain variable region that has the amino acid sequence of SEQ ID NO: 59.
3. The antibody according to claim 1 , wherein the antibody comprises a heavy chain variable region that has the amino acid sequence of SEQ ID NO: 60.
4. An article comprising a stem cell proliferation surface, wherein the stem cell proliferation surface comprises the antibody of claim 1 .
5. The article according to claim 4 , wherein the antibody comprises a kappa chain variable region that has the amino acid sequence of SEQ ID NO: 59.
6. The article according to claim 4 , wherein the antibody comprises a heavy chain variable region that has the amino acid sequence of SEQ ID NO: 60.
7. The article according to claim 4 , wherein the surface comprises nitrogen (N) of about 0.5% or more as determined by surface chemical analysis.
8. The article according to claim 4 , wherein the surface comprises nitrogen (N) of about 1.7% to about 2.1% as determined by surface chemical analysis.
9. The article according to claim 4 , wherein the surface comprises oxygen (O) of about 26.4% to about 28.7% as determined by surface chemical analysis.
10. The article according to claim 4 , wherein the surface comprises a sum of oxygen (O) and nitrogen (N) of about 17.2% or more as determined by surface chemical analysis.
11. The article according to claim 4 , wherein the surface comprises a sum of oxygen (O) and nitrogen (N) of about 28.2% to about 30.7% as determined by surface chemical analysis.
12. The article according to claim 4 , wherein the surface is characterized by a contact angle of at least about 13.9 degrees as determined by surface chemical analysis.
13. The article according to claim 4 , wherein the surface is characterized by a contact angle of about 14.3 to about 18.8 degrees as determined by surface chemical analysis.
14. The antibody according to claim 1 , wherein the antibody comprises a kappa chain variable region that has the amino acid sequence of SEQ ID NO: 59 and a heavy chain variable region that has the amino acid sequence of SEQ ID NO: 60.
15. The article according to claim 4 , wherein the antibody comprises a kappa chain variable region that has the amino acid sequence of SEQ ID NO: 59 and a heavy chain variable region that has the amino acid sequence of SEQ ID NO: 60.
16. The article according to claim 4 , wherein the stem cell proliferation surface does not comprise a feeder layer.
17. The article according to claim 4 , wherein the surface comprises at least about 0.5% Nitrogen (N), at least about a sum of Oxygen (O) and Nitrogen (N) of greater than or equal to 17.2%, and a contact angle of at least about 13.9 degrees.
18. The article according to claim 4 , wherein the surface is a Vita™ surface or a Vita™-like surface.