Methods of protecting a plant from insect pests
A method of controlling or preventing pathogenic damage and/or pest damage in a plant propagation material, a plant, part of a plant and/or plant organ, comprising applying on the plant, part of the plant, plant organ, plant propagation material or a surrounding area thereof a phytoprotective agent comprising an enzyme and an insecticide.
1 . A method comprising treating a plant or plant part with a composition comprising an effective amount of a phytoprotective agent, said phytoprotective agent exhibiting lipase activity and comprising a polypeptide having an amino acid sequence that is at least 70 percent identical to the amino acid sequence set forth herein as SEQ ID NO: 1.
2 . The method of claim 1 , wherein the composition is applied to said plant or plant part in an amount effective to prevent infection and/or infestation of said plant or plant part by one or more pests.
3 . The method of claim 1 , wherein said phytoprotective agent exhibiting lipase activity and comprising a polypeptide having an amino acid sequence that is at least 75 percent identical to the amino acid sequence set forth herein as SEQ ID NO: 1.
4 . The method of claim 1 , wherein said phytoprotective agent exhibiting lipase activity and comprising a polypeptide having an amino acid sequence that is at least 80 percent identical to the amino acid sequence set forth herein as SEQ ID NO: 1.
5 . The method of claim 1 , wherein the phytoprotective agent comprises a polypeptide having an amino sequence that is at least 85 percent identical to the amino acid sequence set forth herein as SEQ ID NO: 1.
6 . The method of claim 1 , wherein the phytoprotective agent is derived from a Thermomyces lanuginosus.
7 . The method of claim 1 , wherein the phytoprotective agent further comprises one or more carbamates, diamides, macrocyclic lactones, neonicotinoids, organophosphates, phenylpyrazoles, pyrethrins, spinosyns, synthetic pyrethroids, tetronic acids and/or tetramic acids aromatic hydrocarbons, benzimidazoles, benzothiadiazole, carboxamides, carboxylic acid amides, morpholines, phenylamides, phosphonates, quinone outside inhibitors, thiazolidines, thiophanates, thiophene carboxamides and/or triazoles.
8 . The method of claim 1 , wherein the composition is foliarly applied to a plant.
9 . The method of claim 1 , wherein the phytoprotective agent is present in said composition in an amount ranging from about 0.001 to about 1% of said composition (by weight, based upon the total weight of said composition.
10 . The method of claim 1 , wherein the phytoprotective agent is present in said composition in an amount ranging from about 0.01 to about 10% of said composition (by weight, based upon the total weight of said composition.
11 . The method of claim 1 , wherein the composition comprises a granule comprising said phytoprotective agent.
12 . The method of claim 1 , wherein the composition comprises a plurality of granules having an average particle size of 20-2000 μm equivalent spherical diameter.
13 . The method of claim 1 , wherein the composition is applied 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, and/or 100 or more days after planting.
14 . A method of controlling or preventing damage from a lepidoptera pest in a plant comprising applying an effective amount of a phytoprotective agent to the plant, said phytoprotective agent comprising an organophosphate and an enzyme exhibiting lipase activity and comprising a polypeptide having an amino acid sequence that is at least 70 percent identical to the amino acid sequence set forth herein as SEQ ID NO: 1.
15 . The method of claim 14 , wherein the enzyme has lipase activity and the organophosphate is a chlorpyrifos.
16 . The method of claim 14 , wherein the enzyme is applied at a concentration of at least about 0.03%, 0.04%, 0.05%, 0.06%, and the organophosphate is applied at a concentration of at least about 15, 16, 17, or 18 mg/L.
17 . A method of controlling or preventing damage from an insect pest in a plant comprising applying an effective amount of a phytoprotective agent to the plant, said phytoprotective agent comprising a neonicotinoid and an enzyme exhibiting lipase activity and comprising a polypeptide having an amino acid sequence that is at least 70 percent identical to the amino acid sequence set forth herein as SEQ ID NO: 1.
18 . The method of claim 17 , wherein the enzyme has protease activity and the neonicotinoid is a clothianidin.
19 . The method of claim 17 , wherein the neonicotinoid is applied at a concentration of at least about 28, 29, 30, 31, or 32 mg/L.