Nucleotide sequences and corresponding polypeptides conferring modified phenotype characteristics in plants
Methods and materials for modulating low-nitrogen tolerance levels in plants are disclosed. For example, nucleic acids encoding low nitrogen tolerance-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased [RCL2] low-nitrogen tolerance levels and plant products produced from plants having increased low-nitrogen tolerance levels.
1. A method of increasing level of low-nitrogen tolerance in a plant, said method comprising
growing plant cells transformed with an exogenous nucleic acid molecule, said exogenous nucleic acid molecule comprising a heterologous promoter operably linked to a polynucleotide sequence encoding a polypeptide having 95 percent or greater amino acid sequence identity to the amino acid sequence of SEQ ID NO: 769; and
producing plants from said transformed plant cells; and
selecting for a transformed plant from said transformed plants that overexpresses said polypeptide and exhibits an increased level of low-nitrogen tolerance as compared to the corresponding level of low-nitrogen tolerance of a control plant of the same species that does not comprise said exogenous nucleic acid molecule.
2. The method of claim 1 , wherein the polynucleotide sequence has 95 percent or greater nucleotide sequence identity to the nucleotide sequence of SEQ ID NO: 768.
3. The method of claim 1 , wherein said polypeptide comprises the amino acid sequence of SEQ ID NO: 769.
4. A transgenic plant cell transformed with an exogenous nucleic acid molecule, said exogenous nucleic acid molecule comprising a heterologous promoter operably linked to a polynucleotide sequence encoding a polypeptide having 95 percent or greater amino acid sequence identity to the amino acid sequence of SEQ ID NO: 769, wherein a transformed plant comprising said transformed plant cell has been obtained and selected for having an increased level of low-nitrogen tolerance as compared to the corresponding level of low-nitrogen tolerance of a control plant of the same species that does not comprise said nucleic acid molecule.
5. The plant cell of claim 4 , wherein the polynucleotide sequence has 95 percent or greater nucleotide sequence identity to the nucleotide sequence of SEQ ID NO: 768.
6. A transgenic plant comprising the transformed plant cell of claim 4 .
7. The transgenic plant of claim 6 , wherein said plant is a member of a species selected from the group consisting of Panicum virgatum (switchgrass), Sorghum bicolor (sorghum, sudangrass), Miscanthus giganteus ( miscanthus ), Saccharum sp. (energycane), Populus balsamifera (poplar), Zea mays (corn), Glycine max (soybean), Brassica napus (canola), Triticum aestivum (wheat), Gossypium hirsutum (cotton), Oryza sativa (rice), Helianthus annuus (sunflower), Medicago sativa (alfalfa), Beta vulgaris (sugarbeet), and Pennisetum glaucum (pearl millet).
8. A product comprising a transgenic plant tissue from the transgenic plant according to claim 7 , and wherein said product comprises said exogenous nucleic acid molecule.
9. A transgenic seed produced by the transgenic plant of claim 6 , and wherein said transgenic seed comprises said exogenous nucleic acid molecule.