Cyanobacteria having improved photosynthetic activity
This disclosure describes modified photosynthetic microorganisms, including Cyanobacteria, that have a reduced amount of a light harvesting protein (LHP) and contain one or more introduced or overexpressed polynucleotides encoding one or more enzymes associated with lipid biosynthesis, and which are capable of producing increased amounts of fatty acids and/or synthesizing triglycerides.
1. A cell culture comprising a population of modified Cyanobacteria cells comprising genetic modifications including:
(a) an addition of an endogenous NbIA gene, addition of an exogenous NbIA gene, or addition of a promoter that regulates NbIA gene expression,
wherein the genetic modification results in changes that, as compared to corresponding wild-type Cyanobacteria, include:
an increased level of NbIA polypeptide or a biologically active fragment of NbIA polypeptide that includes a conserved domain,
an enhanced level of photosynthetic activity, and
a reduced amount of a light harvesting protein (LHP); and
(b) an addition of a nucleic acid sequence comprising an additional exogenous gene encoding an exogenous polypeptide.
2. The cell culture of claim 1 wherein the Cyanobacteria cell culture is cultured under light intensities between about
(a) 200 micromol photons per square meter per second, and
(b) 2000 micromol photons per square meter per second, and
wherein the genetically modified Cyanobacteria cell culture expresses the exogenous polypeptide.
3. The cell culture of claim 1 wherein the exogenous polypeptide is a therapeutic polypeptide.
4. The cell culture of claim 2 wherein the exogenous polypeptide is a therapeutic polypeptide.