Granted Patent
B2
US 12,447,202 · App. 17/056,306 · Granted Oct 21, 2025
Arthrospira platensis oral vaccine delivery platform
Assignee:
Lumen Bioscience, Inc.
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Abstract
The present disclosure provides oral antigenic compositions comprising a recombinant Spirulina comprising at least one exogenous antigenic epitope. Oral antigenic compositions of the present disclosure can be used as vaccines. Oral antigenic compositions of the present disclosure can be used to induce a protective immune response to infectious microorganism, tumor antigens, or self-antigens.
Claims (15)
1. An oral antigenic composition comprising:
a recombinant Spirulina , wherein the recombinant Spirulina comprises an exogenous nucleic acid sequence, encoding:
a chimeric protein comprising an antigenic epitope translationally fused to a viral protein or a virus-like particle (VLP)-forming protein wherein the exogenous nucleic acid sequence is stably integrated into an endogenous locus of the Spirulina ; and
one or more pharmaceutically acceptable excipients.
2. The oral antigenic composition of claim 1 , wherein the antigenic epitope is a self-antigen associated with an autoimmune disease.
3. The oral antigenic composition of claim 1 , wherein the antigenic epitope is from an infectious microorganism.
4. The oral antigenic composition of claim 3 , wherein the infectious microorganism is a bacterium selected from the group consisting of: Mycobacterium, Streptococcus, Staphylococcus, Shigella, Campylobacter, Salmonella, Clostridium, Corynebacterium, Pseudomonas, Neisseria, Listeria, Vibrio, Bordetella , and Legionella.
5. The oral antigenic composition of claim 3 , wherein the infectious microorganism is a virus selected from the group consisting of: bacteriophage, RNA bacteriophage, Infectious Haematopoietic Necrosis Virus, Parvovirus, Herpes Simplex Virus, Hepatitis A virus, Hepatitis B virus, Hepatitis C virus, Measles virus, Mumps virus, Rubella virus, HIV, Influenza virus, Rhinovirus, Rotavirus A, Rotavirus B, Rotavirus C, Respiratory Syncytial Virus (RSV), Varicella zoster, Poliovirus, Norovirus, Zika Virus, Dengue Virus, Rabies Virus, Newcastle Disease Virus, and White Spot Syndrome Virus.
6. The oral antigenic composition of claim 3 , wherein the infectious microorganism is a parasite selected from the group consisting of: Plasmodium, Trypanosoma, Toxoplasma, Giardia, Leishmania Cryptosporidium , helminthic parasites: Trichuris spp., Enterobius spp., Ascaris spp., Ancylostoma spp., Necator spp., Strongyloides spp., Dracunculus spp., Onchocerca spp., Wuchereria spp., Taenia spp., Echinococcus spp., Diphyllobothrium spp., Fasciola spp., and Schistosoma spp.
7. The oral antigenic composition of claim 6 , wherein the infectious microorganism is the Plasmodium , and wherein the Plasmodium is P. falciparum, P. malariae, P. ovale or P. vivax.
8. The oral antigenic composition of claim 1 , wherein the antigenic epitope is from a Plasmodium antigen selected from the group consisting of: circumsporozoite protein, thrombospondin-related anonymous protein (TRAP), Apical Membrane Antigen 1 (AMA1), major merozoite surface proteins 1-3 (MSP1-3), sexual stage antigen 25 (s25), sexual stage antigen s230, and a sequence of NANP (SEQ ID NO: 6), NVDP (SEQ ID NO:7), or NPDP (SEQ ID NO: 8).
9. The oral antigenic composition of claim 1 , wherein the viral protein or VLP-forming protein comprises a capsid protein of a virus.
10. The oral antigenic composition of claim 9 , wherein the capsid protein is hepatitis B core antigen (HbcAg) or woodchuck hepadnaviridae core antigen (WhcAg).
11. The oral antigenic composition of claim 1 , wherein the chimeric protein comprises a scaffold protein, and wherein the antigenic epitope is linked to the scaffold protein at the N-terminus or the C-terminus, or in the body of the scaffold protein.
12. The oral antigenic composition of claim 1 , wherein the recombinant Spirulina is non-living, dried, spray dried, freeze-dried, or lyophilized.
Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jan 14, 2022
From: ROBERTS, JAMES; TASCH, MICHAEL A.; SAVERIA, TRACY
To: LUMEN BIOSCIENCE, INC.
Reel/Frame 058656/0014 →
Continuity (2)
Provisional Application
62672891
· May 17, 2018
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