Formation of array of membranes and apparatus therefor
An array of membranes comprising amphipathic molecules is formed using an apparatus comprising a support defining an array of compartments. Volumes comprising polar medium are provided within respective compartments and a layer comprising apolar medium is provided extending across the openings with the volumes. Polar medium is flowed across the support to displace apolar medium and form a layer in contact with the volumes, forming membranes comprising amphipathic molecules at the interfaces. In one construction of the apparatus, the support that comprises partitions which comprise inner portions and outer portions. The inner portions define inner recesses without gaps therebetween that are capable of constraining the volumes comprising polar medium contained in neighbouring inner recesses from contacting each other. The outer portions extend outwardly from the inner portions and have gaps allowing the flow of an apolar medium across the substrate.
1. A method of forming an array of membranes comprising amphipathic molecules, the method comprising:
providing an apparatus ( 1 ) comprising a support ( 3 ) that comprises partitions ( 6 ) defining an array of compartments ( 4 ), the partitions ( 6 ) comprising inner portions ( 20 ) and outer portions ( 21 ), the inner portions ( 20 ) defining inner recesses ( 22 ) without gaps therebetween that are capable of constraining volumes ( 2 ) comprising polar medium that may be contained in neighboring inner recesses ( 22 ) from contacting each other, and the outer portions ( 21 ) extending outwardly from the inner portions ( 20 ) and having gaps allowing the flow of an apolar medium between the compartments ( 4 ), whereby the compartments ( 4 ) have openings through which polar medium may be introduced;
disposing polar medium and apolar medium onto the support ( 3 ) to provide volumes ( 2 ) comprising polar medium within respective compartments ( 4 ) so that the volumes ( 2 ) comprising polar medium are constrained from contacting volumes ( 2 ) comprising polar medium in neighboring compartments ( 4 ), and a layer comprising apolar medium extending across the openings in the support ( 3 ) in contact with the volumes ( 2 ) comprising polar medium; and
flowing polar medium across the openings in the support ( 3 ) to displace apolar medium and form a layer ( 50 ) comprising polar medium extending across the openings in the support ( 3 ) in contact with the volumes ( 2 ) comprising polar medium and membranes comprising amphipathic molecules at the interfaces between the layer ( 50 ) comprising polar medium and the volumes ( 2 ) comprising polar medium.
2. A method according to claim 1 , wherein the step of disposing polar medium and apolar medium onto the support ( 3 ) comprises:
disposing polar medium onto the support ( 3 ) so that the polar medium enters into the compartments ( 4 ) through the openings; and
subsequently providing said layer comprising apolar medium.
3. A method according to claim 2 , wherein the step of disposing polar medium onto the support ( 3 ) comprises flowing polar medium across the support ( 3 ) so that the polar medium enters into the compartments ( 4 ) through the openings.
4. A method according to claim 3 , wherein the step of disposing polar medium and apolar medium onto the support ( 3 ) comprises, between said step of flowing polar medium across the support ( 3 ) and said step of providing said layer comprising apolar medium, flowing a gas across the support ( 3 ) to displace excess polar medium, leaving the volumes ( 2 ) comprising polar medium in the compartments.
5. A method according to claim 4 , wherein the step of providing said layer comprising apolar medium comprises flowing apolar medium across the support ( 3 ) to displace excess polar medium leaving the volumes ( 2 ) comprising polar medium in the compartments ( 4 ) and the layer comprising apolar medium extending across the openings in the support ( 3 ) in contact with the volumes ( 2 ) comprising polar medium.
6. A method according to claim 2 , further comprising pre-treating the support ( 3 ) with a pre-treatment of apolar medium before said step of disposing polar medium and apolar medium onto the support ( 3 ).
7. A method according to claim 2 , wherein the layer comprising apolar medium further comprises the amphipathic molecules or the polar medium that is flowed across the openings in the support ( 3 ) further comprises the amphipathic molecules.