Granted Patent B2

US 12,458,945 · App. 17/365,517 · Granted Nov 4, 2025

Inventors: Jason Robert Hyde (Oxford, GB); Pedro Miguel Ortiz Bahamon (Oxford, GB); Clive Gavin Brown (Cambridge, GB); Andrew John Heron (Oxford, GB); Paul Raymond Mackett (Oxford, GB)

Assignee: Oxford Nanopore Technologies PLC

B01J19/0046B01L3/5088C12Q1/6869G01N33/48721G01N33/573B01J2219/00313B01J2219/00317B01J2219/00351B01J2219/00585B01J2219/00659B01J2219/00734B01J2219/00736B01L2200/0642B01L2400/086G01N2333/974

Abstract

Claims (31)

1 . An apparatus for forming an amphiphilic membrane, the apparatus comprising:

a substrate comprising a recess, wherein the recess is configured to contain polar medium, and wherein the recess opens at a surface of the substrate; and

pillars, wherein the pillars extend from the surface of the substrate and extend parallel to an axis normal to the surface of the substrate, and wherein gaps between the pillars are configured to allow flow of an apolar medium;

wherein the pillars are configured to support an amphiphilic membrane arranged to contact polar medium contained in the recess; and

wherein the pillars are set back from edges of the recess as viewed from the opening and positioned opposed to one another around the recess.

2 . The apparatus of claim 1 , further comprising a volume of polar medium contained in the recess.

3 . The apparatus of claim 2 , further comprising a layer comprising apolar medium extending across the substrate in contact with the volume of polar medium.

4 . The apparatus of claim 2 , further comprising: a layer comprising polar medium extending across the substrate, wherein the layer comprising polar medium is in contact with the volume of polar medium; an apolar medium that is present around the pillars; and a membrane comprising amphiphilic molecules, wherein the membrane is present at the interface between the layer comprising polar medium and the volume of polar medium.

5 . The apparatus of claim 4 , wherein the membrane comprises a nanopore.

6 . The apparatus of claim 4 , further comprising an electrode in electrical contact with the layer comprising polar medium.

7 . The apparatus of claim 1 , wherein a diameter of the opening of the recess is less than the distance between pillars on opposing sides of the recess.

8 . The apparatus of claim 1 , comprising a third pillar, wherein three pillars surround the recess, wherein each of the pillars has a tri-star shape.

9 . The apparatus of claim 8 , wherein each of the pillars has three arms with curved re-entrant sides and enlarged ends.

10 . The apparatus of claim 8 , wherein the recess has a circular opening and each of the pillars is arranged concentrically around the recess.

11 . The apparatus of claim 8 , wherein the three pillars are equally spaced around the recess.

12 . The apparatus of claim 1 , wherein the recess comprises a base.

13 . The apparatus of claim 1 , wherein the substrate comprises a plurality of recesses arranged in an array, of which the recess is a member of the array of recesses.

14 . The apparatus of claim 13 , wherein the plurality of recesses comprises at least 18 recesses.

15 . The apparatus of claim 13 , wherein the array has a regularly repeating pattern of recesses.

16 . The apparatus of claim 15 , wherein the recesses have the same size and shape as one another.

17 . The apparatus of claim 13 , wherein pillars surround each recess.

18 . The apparatus of claim 17 , wherein at least one pillar is shared by two or more adjacent recesses.

19 . The apparatus of claim 17 , wherein the pillars comprise a regular array of pillars.

20 . The apparatus of claim 19 , wherein the pillars have the same size and shape as one another.

21 . The apparatus of claim 13 , wherein each recess is configured to contain polar medium, and wherein each recess opens at the surface of the substrate.

22 . The apparatus of claim 13 , further comprising volumes of polar medium contained in the recesses.

23 . The apparatus of claim 22 , further comprising a layer comprising apolar medium extending across the substrate in contact with the volumes of polar medium.

24 . The apparatus of claim 22 , further comprising: a layer comprising polar medium extending across the substrate, wherein the layer comprising polar medium is in contact with the volumes of polar medium; an apolar medium that is present around the pillars; and membranes comprising amphiphilic molecules, wherein the membranes are present at the interfaces between the layer comprising polar medium and the volumes of polar medium.

25 . The apparatus of claim 24 , wherein the membranes comprise nanopores.

26 . The apparatus of claim 24 , further comprising a common electrode in electrical contact with the layer comprising polar medium.

27 . The apparatus of claim 13 , wherein each recess comprises a base.

Assignments (2)

Continuity (4)

References Cited (349)