Granted Patent B2

US 12,667,839 · App. 18/103,815 · Granted Jun 30, 2026

Inventor: David Waterman (Oxford, GB)

Assignee: Oxford Nanopore Technologies PLC

B01L3/502715B01L3/502738B01L3/502746B01L2200/0621B01L2200/0684B01L2300/0636B01L2300/0645B01L2300/0816B01L2300/087B01L2300/14B01L2300/161B01L2300/165B01L2400/0406B01L2400/0644

Abstract

Claims (44)

1 . A microfluidic device for analyzing a test liquid comprising:

a sensor provided in a sensing chamber;

a flow path comprising a sensing chamber inlet and a sensing chamber outlet connecting to the sensing chamber for respectively passing liquid into and out of the sensing chamber, and

a sample input port in fluid communication with the sensing chamber inlet;

a liquid collection channel downstream of the sensing chamber outlet;

a flow path interruption between the sensing chamber outlet and the liquid collection channel, preventing liquid from flowing into the liquid collection channel from upstream, whereby the device may be activated by completing the flow path between the sample input port and the liquid collection channel;

a conditioning liquid filling from the sample input port to the flow path interruption such that the sensor is covered by liquid and unexposed to a gas or gas/liquid interface;

wherein the device is configured such that following activation of the device, the sensor remains unexposed to a gas or gas/liquid interface and the application of respectively one or more volumes of test liquid to a wet surface of the sample input port provides a net driving force sufficient to introduce the one or more volumes of test liquid into the device and displace buffer liquid into the liquid collection channel,

wherein the test liquid is unprocessed; and

wherein the device further comprises a removable plug for the sample input port,

wherein the plug is configured to avoid any fluid ingress or egress through the sample input port.

2 . The microfluidic device of claim 1 wherein prior to activation, the buffer liquid fills from the sample input port to the flow path interruption.

3 . The microfluidic device according to claim 1 wherein the sample input port is configured to provide the net driving force.

4 . The microfluidic device according to claim 3 wherein the sample input port is configured so as to facilitate a change in shape of the volume of liquid applied to the sample input port, wherein the net driving force comprises Laplace pressure.

5 . The microfluidic device according to claim 1 , wherein following activation of the device or the introduction of one or more volumes of test liquid, the pressure at the sample input port is substantially equal and opposite to the pressure at the liquid collection channel.

6 . The microfluidic device according to claim 1 , wherein following activation of the device or the introduction of one or more volumes of test liquid, the interfaces at respectively the sensing chamber inlet and the sensing chamber, and the sensing chamber and the sensing chamber outlet, are configured to avoid draining of liquid from the sensing chamber inlet or the sensing chamber outlet out of the sensing chamber so as to avoid the provision of a gas/liquid interface in the sensing chamber.

7 . The microfluidic device of claim 1 , further comprising an activation system operable to activate the device.

8 . The microfluidic device of claim 7 , wherein the flow path interruption comprises a closed valve; and the activation system comprises a mechanism for opening the valve.

9 . The microfluidic device of claim 1 wherein the sensor comprises an array of wells, wherein each well comprises a liquid and wherein a membrane is provided across the surface of each well separating the liquid contained in the well from the conditioning liquid in the sensing chamber.

10 . The microfluidic device of claim 9 , wherein each membrane further comprises a nanopore.

11 . The microfluidic device of claim 10 , wherein the nanopore is a biological nanopore.

12 . A method of filling the microfluidic device of claim 1 with test liquid, the method comprising:

activating the device by completing the flow path between the sensing chamber outlet and the downstream liquid collection channel;

respectively applying one or more volumes of test sample to the wet surface of the sample input port in liquid communication with the downstream liquid collection channel so as to introduce the test liquid into the device,

wherein the test sample is unprocessed.

13 . The method of claim 12 wherein following activation of the device and prior to the introduction of the one or more volumes of test sample, the device is primed to provide a wet surface at the sample input port in liquid communication with the sensing chamber inlet.

14 . The method of claim 12 wherein the device is primed following removal of the plug for the sample input port.

15 . The method of claim 14 , wherein the step of priming comprises providing priming liquid to the device through the sample input port.

16 . The method of claim 14 , wherein the step of priming comprises drawing fluid from inside the device into the sample input port.

17 . The method of claim 12 wherein a plurality of discrete volumes of test liquid are successively applied to the sample input port in order to successively displace buffer liquid into the liquid collection channel.

18 . The microfluidic device of claim 1 , wherein the test liquid comprises blood.

19 . The method of claim 12 , wherein the test sample comprises blood.

20 . A diagnostic device for analyzing a test liquid from a patient comprising:

a sensor provided in a sensing chamber;

a flow path comprising a sensing chamber inlet and a sensing chamber outlet connecting to the sensing chamber for respectively passing liquid into and out of the sensing chamber, and

a sample input port in fluid communication with the sensing chamber inlet;

a liquid collection channel downstream of the sensing chamber outlet;

a flow path interruption between the sensing chamber outlet and the liquid collection channel, preventing liquid from flowing into the liquid collection channel from upstream, whereby the device may be activated by completing the flow path between the sample input port and the liquid collection channel;

a conditioning liquid filling from the sample input port to the flow path interruption such that the sensor is covered by liquid and unexposed to a gas or gas/liquid interface;

wherein the device is configured such that following activation of the device, the sensor remains unexposed to a gas or gas/liquid interface and the application of respectively one or more volumes of the test liquid to a wet surface of the sample input port provides a net driving force sufficient to introduce the one or more volumes of test liquid into the device and displace buffer liquid into the liquid collection channel,

wherein the test liquid from the patient is unprocessed, and

wherein the device further comprises a removable plug for the sample input port,

wherein the plug is configured to avoid any fluid ingress or egress through the sample input port.

21 . The diagnostic device of claim 20 , wherein the test liquid comprises blood.

Assignments (2)

Priority Claims (1)

Continuity (2)

References Cited (375)