Parallel flow cytometer using radiofrequency multiplexing
An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.
1. A method comprising:
irradiating with a light beam generator a sample in a flow stream comprising particles;
detecting light from the irradiated particles in the sample with a photodetector;
generating one or more waveforms from the detected light;
applying a transform to the one or more generated waveforms; and
determining a property of the particles based on the one or more transformed waveforms.
2. The method according to claim 1 , wherein the method comprises applying a Fourier transform to the one or more generated waveforms.
3. The method according to claim 1 , wherein the detected light is fluorescence from the irradiated particle.
4. The method according to claim 3 , wherein the method comprises separating different colors of fluorescence.
5. The method according to claim 4 , wherein the separated colors of fluorescence are associated with different properties of the particles.
6. The method according to claim 1 , wherein the method comprises generating the one or more waveforms by digitizing data signals from the photodetector.
7. The method according to claim 6 , wherein the data signals from the photodetector are digitized using an analog-to-digital converter (ADC), a digitizing oscilloscope or a multi-channel lock-in amplifier.
8. The method according to claim 7 , wherein the data signals from the photodetector are digitized using an analog-to-digital converter (ADC).
9. The method according to claim 1 , wherein the particles comprise cells or components of cells.
10. The method according to claim 1 , wherein the light beam generator comprises:
a laser; and
a frequency shifter component configured to generate from a beam of light from the laser a first beam of frequency shifted light and a second beam of frequency shifted light.
11. The method according to claim 10 , wherein the frequency shifter component comprises one or more of an acousto-optic deflector (AOD) and an acousto-optic frequency shifter (AOFS).
12. The method according to claim 10 , wherein the frequency shifter component comprises an acousto-optical combiner configured to produce an optical interrogation beam comprising the first beam of frequency shifted light and the second beam of frequency shifted light.
13. The method according to claim 12 , wherein the photodetector is configured to simultaneously detect fluorescence from particles at a first modulation frequency and at a second modulation frequency.
14. The method according to claim 13 , wherein the first modulation frequency is different from the second modulation frequency.
15. An apparatus comprising:
a light beam generator for irradiating a sample in a flow stream comprising particles;
a photodetector configured to detect light from irradiated particles in the flow stream;
having memory operably coupled to the processor where the memory includes instructions stored thereon, which when executed by the processor, cause the processor to:
generate a waveform from the detected light;
apply a transform to the generated waveform; and
determine a property of the particles based on the transformed waveform.
16. The apparatus according to claim 15 , wherein the memory includes instructions for applying a Fourier transform to the generated waveform.
17. The apparatus according to claim 15 , wherein the system comprises an analog-to-digital converter (ADC) for generating the one or more waveforms by digitizing data signals from the photodetector.
18. The apparatus according to claim 15 , wherein the light beam generator comprises:
a laser; and
a frequency shifter component configured to generate from a beam of light from the laser a first beam of frequency shifted light and a second beam of frequency shifted light.
19. The apparatus according to claim 18 , wherein the frequency shifter component comprises one or more of an acousto-optic deflector (AOD) and an acousto-optic frequency shifter (AOFS).
20. The apparatus according to claim 18 , wherein the photodetector is configured to simultaneously detect fluorescence from particles at a first modulation frequency and at a second modulation frequency.