Granted Patent B2

US 12,529,023 · App. 17/078,157 · Granted Jan 20, 2026

Inventors: Weston Blaine Griffin (Niskayuna, NY); Xiaohua Zhang (Niskayuna, NY); Reginald Donovan Smith (Schnectady, NY); Vandana Keskar (Schnectady, NY)

Assignee: Global Life Sciences Solutions USA LLC

C12M29/04C12M23/08C12M23/24C12M25/02C12M25/06C12M29/18C12M29/26C12M33/00C12M41/46C12N5/0636

Abstract

Claims (27)

1 . A cell culture vessel for expansion of a cell density, comprising:

a bottom plate comprising a grid surface configured to provide support and gas exchange;

a vessel body coupled to the bottom plate, wherein the vessel body comprises a rigid concave structure;

a cavity formed by the vessel body and the bottom plate;

a gas-permeable, liquid impermeable membrane disposed above the bottom plate within the cavity;

a feeding port disposed through a surface of the vessel body, wherein the feeding port is configured to permit addition of cell culture medium into the cavity; and

a drain port disposed through a wall of the vessel body, wherein the drain port is configured to permit removal of used cell culture medium from the cavity;

wherein the cell culture vessel is configured to hold a suspension comprising cells suspended in the cell culture medium, and wherein the cell culture medium within the cell culture vessel comprises a ratio of cell culture medium volume per surface area of the gas permeable, liquid impermeable membrane that is below a predetermined threshold value; and

wherein the drain port is disposed below a surface of the cell culture medium within the cavity and at or above the gas permeable, liquid impermeable membrane when the cavity contains the cell culture medium and the cells, and wherein the drain port is further configured to permit cell removal from the cavity of the cell culture vessel.

2 . The cell culture vessel of claim 1 , wherein the cell culture vessel comprises an angled side, wherein the angled side comprises the side wall of the vessel body and a corresponding side of the bottom plate, wherein the angled side comprises a point, and wherein the drain port is disposed at the point of the angled side.

3 . The cell culture vessel of claim 1 , wherein the threshold value comprises a ratio of cell culture medium volume per surface area of the gas permeable, liquid impermeable membrane between 0.3 and 2.

4 . The cell culture vessel of claim 1 , wherein the drain port is further configured to permit removal of cells from the cavity.

5 . The cell culture vessel of claim 1 , wherein a constant volume is maintained in the cell culture vessel until the cells expand to a desired cell density.

6 . The cell culture vessel of claim 1 , wherein cell culture medium is introduced through the feeding port and removed through the drain port simultaneously.

7 . The cell culture vessel of claim 1 , wherein the cell culture vessel enables cell expansion via perfusion.

8 . The cell culture vessel of claim 7 , wherein the perfusion is continuous.

9 . The cell culture vessel of claim 1 , wherein a volume equal to the volume of the cell culture vessel is exchanged over one day.

10 . The cell culture vessel of claim 1 , wherein the cell culture vessel may be tilted toward the drain port for harvest of cell culture.

11 . The cell culture vessel of claim 1 , wherein the vessel body is transparent.

12 . The cell culture vessel of claim 1 , wherein the bottom plate is formed of a non-solid structure.

13 . The cell culture vessel of claim 1 , wherein the membrane is a cell culture surface.

14 . The cell culture vessel of claim 1 , wherein the membrane has a thickness of less than 0.01 inches.

15 . The cell culture vessel of claim 1 , wherein the membrane comprises silicon.

16 . The cell culture vessel of claim 1 , further comprising a mesh sheet disposed between the bottom plate and the membrane.

17 . The cell culture vessel of claim 16 , wherein the mesh sheet comprises polyester mesh.

18 . The cell culture vessel of claim 1 , further comprising an O-ring to seal the cell culture vessel when the vessel body is coupled to the bottom plate.

19 . The cell culture vessel of claim 1 , further comprising an air filter.

Assignments (2)

Continuity (2)

References Cited (228)