Cell culture methods and devices utilizing gas permeable materials

1. A method of culturing animal cells in a cell culture device

comprising a single compartment, at least the bottom of said single compartment being comprised at least in part of a non porous gas permeable material, said gas permeable material is in contact with ambient gas; the method comprising

adding medium and animal cells into said cell culture device; and

placing said cell culture device in a cell culture location that includes ambient gas at a composition for cell culture, wherein said cell culture device is oriented in a position such that the uppermost location of said medium is more than 2.0 cm higher than the lowest location of said medium, and said device is in a state of static cell culture.

2. The method of claim 1 wherein said medium is at least 2.5 cm higher than the lowest location of said medium.

3. The method of claim 1 wherein said medium is at least 3.2 cm higher than the lowest location of said medium.

4. The method of claim 1 wherein said medium is at least 4.0 cm higher than the lowest location of said medium.

5. The method of claim 1 wherein said medium is at least 5.09 cm higher than the lowest location of said medium.

6. The method of claim 1 wherein said medium is at least 7.0 cm higher than the lowest location of said medium.

7. The method of claim 1 wherein a portion of said medium resides in an area not directly above the cells being cultured.

8. The method of claim 1 wherein said cell culture device is further comprising at least one gas permeable material support in contact with said non porous gas permeable material.

9. The method of claim 1 wherein said compartment includes a first sidewall comprised at least in part of non porous gas permeable material and wherein, at some point in time after said state of static culture has been initiated, said cell culture device is oriented into a position in which cells gravitate to said first sidewall.

10. A method of culturing animal cells comprising:

a. adding medium and animal cells into a cell culture device, the cell culture device comprising a single compartment, at least the bottom of said single compartment being comprised at least in part of non porous gas permeable material that is in contact with ambient gas; and

b. placing said cell culture device in a cell culture location that includes ambient gas at a composition for cell culture, wherein said cell culture device is oriented in a position such that the said bottom is horizontal, the uppermost location of said medium is beyond 2.0 cm above the lowest location of said medium, and said device is in a state of static cell culture.

11. The method of claim 10 wherein said medium is at least 2.5 cm above the lowest location of said medium.

12. The method of claim 10 wherein said medium is at least 3.2 cm above the lowest location of said medium.

13. The method of claim 10 wherein said medium is at least 4.0 cm above the lowest location of said medium.

14. The method of claim 10 wherein said medium is at least 5.09 cm above the lowest location of said medium.

15. The method of claim 10 wherein said medium is at least 7.0 cm above the lowest location of said medium.

16. The method of claim 10 wherein said medium is at least 8.0 cm above the lowest location of said medium.

17. The method of claim 10 wherein said medium is at least 9.0 cm above the lowest location of said medium.

18. The method of claim 10 wherein said medium is at least 10.2 cm above the lowest location of said medium.

19. A method of culturing animal cells in a cell culture device

comprising a single compartment, at least the bottom of said single compartment being comprised at least in part of non porous gas permeable material that is in contact with ambient gas; the method comprising:

adding medium and animal cells into said cell culture device; and

placing said cell culture device in a cell culture location that includes ambient gas at a composition for cell culture, wherein said cell culture device is oriented in a position such that the said bottom is horizontal, the uppermost location of said medium is beyond 2.0 cm above the lowest location of said medium, and said device is and said device is not subjected to mixing or perfusion.

20. The method of claim 19 wherein said medium is at least 2.5 cm above the lowest location of said medium.

21. The method of claim 19 wherein said medium is at least 3.2 cm above the lowest location of said medium.

22. The method of claim 19 wherein said medium is at least 4.0 cm above the lowest location of said medium.

23. The method of claim 19 wherein said medium is at least 5.09 cm above the lowest location of said medium.

24. The method of claim 19 wherein said medium is at least 7.0 cm above the lowest location of said medium.

25. The method of claim 19 wherein said medium is at least 8.0 cm above the lowest location of said medium.

26. The method of claim 19 wherein said medium is at least 9.0 cm above the lowest location of said medium.

27. The method of claim 19 wherein said medium is at least 10.2 cm above the lowest location of said medium.

28. A method of culturing animal cells comprising:

adding medium and animal cells into a cell culture device comprising a single compartment, at least the bottom of said single compartment being comprised at least in part of non porous gas permeable material that is in contact with ambient gas and performing static cell culture with the device in a cell culture location that includes ambient gas at a composition for animal cell culture, wherein said bottom is horizontal, the uppermost location of said medium is beyond 2.0 cm above the lowest location of said medium, and said device is not subjected to mixing or perfusion.

29. The method of claim 28 wherein said medium is at least 3.2 cm above the lowest location of said medium.

30. The method of claim 28 wherein said medium is at least 5.09 cm above the lowest location of said medium.