Granted Patent
B2
US 12,311,053 · App. 17/121,578 · Granted May 27, 2025
Nanotube-vesicle compositions and uses thereof
Inventors:
Aleksandr Noy (Livermore, CA); Nga Thuy Ho (Oakland, CA); Gerhard Hummer (Munich, DE); Marc Siggel (Munich, DE)
Assignees:
LAWRENCE LIVERMORE NATIONAL SECURITY, LLC; THE REGENTS OF THE UNIVERSITY OF CALIFORNIA; MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.
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Abstract
Disclosed herein is an engineered lipid-based vesicle optionally used for delivery of one or more payloads. The vesicle comprises a nanotube dimer or complex embedded with a lipid bilayer of the lipid-based vesicle. Also described herein are compositions, e.g., pharmaceutical compositions, and kits comprising the engineered lipid-based vesicle. In additional embodiments, further described herein are use of the engineered lipid-based vesicle for treating a disease or condition, for delivery to a target, or for labeling of a cell.
Claims (24)
1. A method of fusing lipid bilayers, the method comprising:
contacting (a) an engineered unilamellar liposome comprising a first lipid bilayer and a nanotube dimer embedded in a circumference of the first lipid bilayer with (b) a second lipid bilayer, wherein:
said contacting results in fusing the first lipid bilayer with the second lipid bilayer into a single lipid bilayer,
the nanotube dimer comprises a first nanotube and a second nanotube, which is essentially parallel to the first nanotube, and
the nanotube dimer has an asymmetric cross section perpendicular to a length direction of the first nanotube and the second nanotube, so that the dimer has a wider hydrophobic facet and a narrower facet, each of the wider hydrophobic facet and the narrower facet extends along the length direction of the first nanotube and the second nanotube, and the wider facet has a larger dimension in the asymmetric cross section than the narrower facet.
2. The method of claim 1 , wherein the liposome has a diameter across the major axis of the liposome of at least about 28 nm.
3. The method of claim 1 , wherein the liposome has a diameter across the major axis of the liposome of at least about 300 nm.
4. The method of claim 1 , wherein each of the first nanotube and the second nanotube is a nanotube selected from the group consisting of a carbon nanotube, a boron nitride nanotube, a MoS 2 nanotube, a MoS 2 -carbon nanotube hybrid, and a carbon-MoS 2 —WS 2 nanotube hybrid.
5. The method of claim 1 , wherein each of the first nanotube and the second nanotube is a carbon nanotube.
6. The method of claim 1 , wherein each of the first nanotube and the second nanotube is a single wall carbon nanotube.
7. The method of claim 1 , wherein each of the first nanotube and the second nanotube has an outer diameter of from about 0.7 nm to about 2 nm.
8. The method of claim 1 , wherein each of the first nanotube and the second nanotube a length from about 6 nm to about 30 nm.
9. The method of claim 1 , wherein each of the first nanotube and the second nanotube comprises a terminal COOH group and wherein the dimer comprises a carbodiimide crosslinker linking the first nanotube to the second nanotubes through respective terminal COOH groups.
10. The method of claim 1 , wherein the dimer is formed by reacting a terminal alkyne group on the first nanotube with a terminal azide group of the second nanotube.
11. The method of claim 1 , wherein the second lipid bilayer is a lipid bilayer of a cell.
12. The method of claim 11 , wherein the engineered unilamellar liposome comprises a payload and wherein said fusing delivers the payload to the cell.
13. The method of claim 12 , wherein the payload is a drug.
14. The method of claim 12 , wherein the payload is a small molecule, a protein, a polypeptide, a nucleic acid molecule, a protein conjugate, polypeptide conjugate, a nucleic acid molecule conjugate, a polymer, a dye, or a gene-editing system.
15. The method of claim 14 , wherein the payload is an antitumor agent, an antimicrobial agent, a contrast agent, an antioxidant, or an anti-inflammatory agent.
16. The method of claim 12 , wherein the payload is doxorubicin.
17. The method of claim 12 , wherein the cell is a cancer cell, a cell that is infected with a pathogen, a cell that is associated with an autoimmune disease, or a cell that is associated with an inflammation.
18. The method of claim 1 , wherein the first lipid bilayer comprises one or more phospholipids.
19. The method of claim 18 , wherein the first lipid bilayer further comprises cholesterol.
20. The method of claim 1 , wherein the first lipid bilayer comprises one or more PEG-lipids.
Assignments (4)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Aug 15, 2023
From: HO, NGA THUY
To: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Reel/Frame 064597/0503 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Aug 15, 2023
From: NOY, ALEKSANDR
To: LAWRENCE LIVERMORE NATIONAL SECURITY, LLC
Reel/Frame 064597/0529 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Apr 28, 2021
From: HUMMER, GERHARD; SIGGEL, MARC
To: MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.
Reel/Frame 056073/0686 →
CONFIRMATORY LICENSE (SEE DOCUMENT FOR DETAILS)
Recorded Mar 22, 2021
From: LAWRENCE LIVERMORE NATIONAL SECURITY, LLC
To: U.S. DEPARTMENT OF ENERGY
Reel/Frame 055677/0673 →
Continuity (3)
Continuation
PCTUS2020064707
· Dec 11, 2020
Provisional Application
62948169
· Dec 13, 2019
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