Plant resistant to
The present invention provides an improved Helminthosporium turcicum -resistant plant, in particular a maize plant which comprises a polynucleotide with one or more resistance-conferring genes, for example on a truncated chromosome fragment from the accession Pepitilla, as well as a cell, a tissue, a part, grain and seeds thereof, an isolated polynucleotide which comprises one or more resistance-conferring genes against Helminthosporium turcicum , a vector, a transgenic plant cell and a transgenic plant containing this polynucleotide. Furthermore, the invention encompasses suitable markers and their use in introducing resistance or the transgene into a plant, as well as the identification of improved maize plants which comprise a truncated chromosome fragment.
1. A method for identifying a Helminthosporium turcicum -resistant maize plant, the genome of which maize plant has a chromosome fragment from a donor Pepitilla integrated therein, the method comprising:
detecting at least two alleles in the genome of the plant;
(i) wherein at least one allele is localized in a genomic segment which is flanked by a marker in a first marker region, a second marker region, a third marker region, or a fourth marker region and by a polynucleotide which confers resistance against Helminthosporium turcicum in the maize plant;
(ii) wherein at least one allele is localized in a genomic segment which is flanked by the polynucleotide which confers resistance against Helminthosporium turcicum and by a marker in a sixth marker region or a fifth marker region, and
wherein:
the first marker region is flanked by the markers SYN14136 and PZE-108076510, the second marker region is flanked by the markers SYN24931 and PZE-108077560, the third marker region is flanked by the markers PZE-108093423 and PZE-108093748, the fourth marker region is flanked by the markers MA0004 and MA0005, the fifth marker region is flanked by the markers MA0006 and PZE-108097482, and the sixth marker region is flanked by the markers PZE-108107671 and SYN4196.
2. The method as claimed in claim 1 , wherein the detection of a Helminthosporium turcicum -resistant maize plant comprises the use of an oligonucleotide which comprises a nucleotide sequence selected from the group consisting of SEQ ID NOs: 17-52, 62-64, and 244-246.
3. The method as claimed in claim 1 , wherein the detection of a Helminthosporium turcicum -resistant maize plant comprises the use of an oligonucleotide which comprises a nucleotide sequence selected from the group consisting of SEQ ID NOs: 17-52, wherein the oligonucleotide corresponds to at least one of the first marker region which is flanked by the markers SYN14136 and PZE-108076510, the second marker region which is flanked by the markers SYN24931 and PZE-108077560, the third marker region which is flanked by the markers PZE-108093423 and PZE-108093748, the fourth marker region which is flanked by the markers MA0004 and MA0005, the fifth marker region, which is flanked by the markers MA0006 and PZE-108097482, and the sixth marker region which is flanked by the markers PZE-108107671 and SYN4196.
4. The method as claimed in claim 1 , wherein the chromosome fragment comprising the interval of the donor further comprises the interval of the donor which exhibits at least the donor allele of the marker MA0008.
5. The method as claimed in claim 1 , wherein the chromosome fragment does not comprise:
a) an interval of the donor between a marker in the first marker region which is flanked by the markers SYN14136 and PZE-108076510 and a marker in the second marker region which is flanked by the markers SYN24931 and PZE-108077560,
b) an interval of the donor between a marker in the third marker region which is flanked by the markers PZE-108093423 and PZE-108093748 and a marker in the fourth marker region which is flanked by the markers MA0004 and MA0005, and
c) an interval of the donor between a marker in the fifth marker region which is flanked by the markers MA0006 and PZE-108097482 and a marker in the sixth marker region which is flanked by the markers PZE-108107671 and SYN4196.
6. The method as claimed in claim 1 , wherein the chromosome fragment does not comprise:
a) an interval of the donor defined by the marker SYN14136 and the marker SYN24931;
b) an interval of the donor defined by the marker PZE-108093748 and the marker MA0005; and
c) an interval of the donor defined by the marker MA0006 and the marker PZE-108107671.
7. The method as claimed in claim 1 , wherein the chromosome fragment does not comprise an interval of the donor defined by and including the marker SYN14136 and the marker SYN24931.
8. The method as claimed in claim 1 , wherein the chromosome fragment does not comprise an interval of the donor defined by and including the marker PZE-108093748 and the marker MA0005.
9. The method as claimed in claim 1 , wherein the chromosome fragment does not comprise an interval of the donor defined by and including the marker MA0006 and the marker PZE-108107671.