Apparatus and method for processing samples containing biological cells
An apparatus for the automated processing of samples containing biological cells—in particular, of blood samples or other cell samples is provided. The apparatus has at least the following components: a sample receiving device configured to receive one or more samples; an auxiliary material receiving device configured to receive one or more auxiliary materials; a sample carrier receiving device configured to receive one or more sample carriers; a discharge device configured to discharge one or more samples from a discharge position; and a capture device configured to capture one or more discharged samples in a capture position to obtain one or more prepared samples; wherein the distance between the discharge position and the capture position can be adjusted to a prespecified height, and the apparatus further includes at least one temperature control device for controlling the temperature of at least one of the sample carriers.
1 . A method for an automated processing of a cell-containing sample containing biological cells, the method comprising:
wetting a temperature-controlled sample carrier with at least one auxiliary material, the sample carrier being a microscopic glass slide;
positioning the cell-containing sample in a discharging position at a prespecified height relative to a capture position;
discharging the cell-containing sample from the discharging position onto the wetted temperature-controlled microscopic glass slide positioned at the capture position, wherein the prespecified height is selected such that the biological cells at least partially burst when the biological cells hit the microscopic glass slide, wherein the cell-containing sample travels from the discharge position to the capture position along a discharge path, and wherein a region adjacent to the discharge path is at least partially shielded from said discharge path to prevent contamination with sample sprays; and
immobilizing the at least partially burst biological cells on the microscopic glass slide.
2 . The method according to claim 1 , wherein the sample containing the biological cells is a blood sample or another cell sample.
3 . The method according to claim 1 , further comprising:
cooling the temperature-controlled sample carrier to a temperature below room temperature.
4 . The method according to claim 1 , further comprising:
inclining the temperature-controlled sample carrier at a prespecified angle of inclination relative to a horizontal plane before the sample is discharged onto the temperature-controlled sample carrier.
5 . The method according to claim 1 , further comprising:
cooling the sample to a temperature below room temperature before discharging the sample onto the temperature-controlled sample carrier.
6 . The method according to claim 1 , wherein the sample undergoes an analysis after being discharged onto the temperature-controlled sample carrier.