Granted Patent
B2
US 12,558,395 · App. 17/175,845 · Granted Feb 24, 2026
Stable dry probiotic compositions for special dietary uses
Inventors:
Majid Keshtmand (Baltimore, MD); Mordechai Harel (Pikesville, MD); Trisha D. Rice (Columbia, MD)
Assignee:
Advanced Bionutrition Corp.
A61K38/011A23L2/66A23L33/125A23L33/135A23L33/15A23L33/185A23L33/19A23L33/40A61K9/0056A61K9/0095A61K31/355A61K31/7016A61K31/721A61K31/733A61K31/736A61K35/745A61K35/747A61K38/018A61K47/22A23V2002/00A23V2400/113A23V2400/175A61K2035/115
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Abstract
A dry stable probiotic composition is provided. The composition comprises one or more viable probiotic microorganisms, one or more hydrolyzed proteins, one or more disaccharides, one or more oligosaccharides, and one or more polysaccharides, but not trehalose. The composition has viability of at least 1×10 10 CFU/g, and a viability loss of less than 1 log unit/g after one month at a temperature of 40° C. and a relative humidity of 33%. Also provided are methods for preparing the dry stable probiotic composition.
Claims (23)
1 . A dry composition comprising one or more viable probiotic microorganisms, one or more hydrolyzed proteins at 45-65%, one or more disaccharides at 5-30%, one or more oligosaccharides, and one or more polysaccharides, each percentage based on the total dry weight of the composition, wherein the composition does not comprise trehalose, and wherein the one or more viable probiotic microorganisms are selected from the group consisting of live probiotic bacteria, wherein the probiotic bacteria have viability of at least 1×10 10 CFU/g, and wherein the probiotic bacteria have a viability loss of less than 1 log unit/g after one month at a temperature of 40° C. and a relative humidity of 33%.
2 . The composition of claim 1 , wherein the composition provides a probiotic benefit to a host in a special dietary product.
3 . The composition of claim 2 , wherein the special dietary product is selected from the group consisting of an infant formula, a follow-on formula, processed cereal based food, canned baby food, and special food for a medical purpose.
4 . The composition of claim 2 , wherein the special dietary product is an infant formula.
5 . The composition of claim 1 , wherein the composition comprises the one or more hydrolyzed proteins at 55-60% based on the total dry weight of the composition.
6 . The composition of claim 1 , wherein the one or more hydrolyzed proteins are selected from the group consisting of milk proteins, plant proteins, and combinations thereof.
7 . The composition of claim 1 , wherein the one or more hydrolyzed proteins are selected from the group consisting of hydrolyzed casein, hydrolyzed whey protein, hydrolyzed pea protein, hydrolyzed soy protein, and combinations thereof.
8 . The composition of claim 1 , wherein the composition comprises the one or more disaccharides at 10-25% based on the total dry weight of the composition.
9 . The composition of claim 1 , wherein the one or more disaccharides are selected from the group consisting of sucrose, lactose, and combinations thereof.
10 . The composition of claim 1 , wherein the composition comprises the one or more oligosaccharides at 1-30% based on the total dry weight of the composition.
11 . The composition of claim 1 , wherein the one or more oligosaccharides are selected from the group consisting of inulin, maltodextrins, dextrans, fructo-oligosaccharides (FOS), galacto-oligosaccharides (GOS), mannan-oligosaccharides (MOS), and combinations thereof.
12 . The composition of claim 1 , wherein the composition comprises of the one or more polysaccharides at 0.1-40% based on the total dry weight of the composition.
13 . The composition of claim 1 , wherein the one or more polysaccharides are selected from the group consisting of carrageenan, guar gum, gum acacia, locust bean gum, starches, modified starches, and combinations thereof.
14 . The composition of claim 1 , further comprising one or more additional agents.
15 . The composition of claim 14 , wherein the composition comprises the one or more additional agents at 0.5-20% based on the total dry weight of the composition.
16 . The composition of claim 14 , wherein the one or more additional agents are selected from the group consisting of carboxylic acid salts, tocopherols, and combinations thereof.
17 . The composition of claim 16 , wherein the carboxylic acid salts are selected from the group consisting of ascorbic acid salts and citric acid salts.
18 . The composition of claim 14 , wherein the one or more additional agents comprise one or more tocopherols and one or more carboxylic acid salts at a weight ratio from 1:4 to 4:1.
19 . A method for preparing the composition of claim 1 , comprising:
(a) combining the one or more viable probiotic microorganisms, the one or more hydrolyzed proteins, the one or more disaccharides, the one or more oligosaccharide, and the one or more polysaccharides in an alkali aqueous solvent to form a slurry;
(b) snap-freezing the slurry in liquid nitrogen to form solid frozen particles in the form of beads, droplets or strings;
(c) primary drying step of the solid frozen particles by evaporation, under vacuum, while maintaining the temperature of the particles above their freezing temperature, whereby a primarily dried formulation is formed; and
(d) secondary drying of the primarily dried formulation at full strength vacuum and a heat source temperature of 20° C. or higher for a time sufficient to reduce the water activity of the primarily dried formulation to 0.3 Aw or lower, whereby the composition of claim 1 is prepared.
Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Feb 15, 2021
From: KESHTMAND, MAJID; HAREL, MORDECHAI; RICE, TRISHA D.
To: ADVANCED BIONUTRITION CORP.
Reel/Frame 055260/0106 →
Continuity (3)
Continuation In Part
15747579
Provisional Application
62198220
· Jul 29, 2015
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