Granted Patent B2

US 12,629,683 · App. 17/200,538 · Granted May 19, 2026

Inventors: Kandaswamy Vijayan (San Diego, CA); Andrew Boyd Macconnell (San Diego, CA); Joseph Franklin Rokicki (Del Mar, CA); Michael Van Nguyen (San Diego, CA)

Assignee: Plexium, Inc.

B01L3/50853C12N15/1034C12N15/1065C12N15/1068C12N15/1075C12Q1/6869C12Q1/6876C40B30/04C40B30/06C40B50/04C40B50/14C40B50/16B01L2300/0829C12Q2600/16

Abstract

Claims (40)

1 . A bead complex comprising:

a bead;

a plurality of same compounds directly bound to the bead at a first position via a cleavable linker; and

directly bound to the bead at a second position different from the first position, in a sequential order:

a linker;

a primer directly bound to the linker;

an encoding region comprising a single-stranded DNA barcode, wherein the single-stranded DNA barcode is directly bound to the primer; and

a nucleic acid capturing group comprising a poly-T sequence, the nucleic acid capturing group being single-stranded and directly bound to the single-stranded DNA barcode, wherein:

the encoding region encodes a structure of each same compound of the plurality of same compounds or synthetic steps used to make the each corresponding same compound.

2 . The bead complex of claim 1 , wherein the cleavable linker comprises a photo-cleavable linker, an acid cleavable linker, a base cleavable linker, or a temperature cleavable linker.

3 . The bead complex of claim 1 , wherein the plurality of the same compounds each comprise multiple compound building blocks.

4 . The bead complex of claim 3 , wherein the encoding region encodes the synthetic steps used to make the each same compound.

5 . The bead complex of claim 1 , wherein the nucleic acid capturing group is configured to capture messenger RNA (mRNA).

6 . The bead complex of claim 1 , wherein the nucleic acid capturing group is configured to capture microRNA (miRNA).

7 . A plurality of bead complexes comprising a first bead complex and a second bead complex, the first bead complex comprising:

a first bead;

a first plurality of same compounds directly bound to the first bead at a first position via a first cleavable linker; and

directly bound to the first bead at a second position different from the first position, in a sequential order:

a linker;

a first primer directly bound to the first linker;

a first encoding region comprising a first single-stranded DNA barcode, wherein the first single-stranded DNA barcode is directly bound to the first primer; and

a first nucleic acid capturing group comprising a first poly-T sequence, the first nucleic acid capturing group being single-stranded and directly bound to the first single-stranded DNA barcode, wherein:

the first encoding region encodes a structure of each first same compound of the first plurality of same compounds or synthetic steps used to make the each first same compound; and the second bead complex comprising:

a second bead;

a second plurality of second same compounds directly bound to the second bead at a third position via a second cleavable linker; and

directly bound to the second bead at a fourth position different from the third position, in a sequential order:

a second linker;

a second primer directly bound to the second linker;

a second encoding region comprising a second single-stranded DNA barcode, wherein the second single-stranded DNA barcode is directly bound to the first primer; and

a second nucleic acid capturing group comprising a second poly-T sequence, the second nucleic acid capturing group being single-stranded and directly bound to the second single-stranded DNA barcode, wherein:

the second encoding region encodes a structure of each second same compound of the second plurality of same compounds or synthetic steps used to make the each second same compound, the second plurality of second same compounds are different from the first plurality of first same compounds, and the second single-stranded DNA barcode is different from the first single-stranded DNA barcode.

8 . The plurality of bead complexes of claim 7 , wherein the first cleavable linker comprises a photo-cleavable linker, an acid cleavable linker, a base cleavable linker, or a temperature cleavable linker.

9 . The plurality of bead complexes of claim 7 , wherein the first plurality of the first same compounds comprise multiple compound building blocks.

10 . The plurality of bead complexes of claim 7 , wherein the first nucleic acid capturing group is configured to capture messenger RNA (mRNA).

11 . The plurality of bead complexes of claim 7 , wherein the first nucleic acid capturing group is configured to capture microRNA (miRNA).

12 . The bead complex of claim 1 , wherein the each same first compound comprises a benzimidazole compound, imidazolidinone compound, quinazolinone compound, isoindolinone compound, thiazole compound, or an imidazopyridine compound.

13 . The bead complex of claim 1 , wherein the each same first compound comprises a lenalidomide or a stereoisomer or an enantiomer thereof.

14 . The bead complex of claim 1 , wherein the plurality of same compounds are screened for activity of inhibiting or activating a protein or a cellular function.

15 . The bead complex of claim 1 , wherein the plurality of same compounds comprise non-nucleic acid molecules.

16 . The bead complex of claim 1 , wherein the plurality of same compounds comprise amino acids.

Assignments (1)

Continuity (5)

References Cited (199)