IP Library › Granted Patent US 12,391,624
Granted Patent B2
US 12,391,624 · App. 17/258,024 · Granted Aug 19, 2025

Temporally and spatially targeted dynamic nitrogen delivery by remodeled microbes

Inventors: Alvin Tamsir (Berkeley, CA); Sarah Bloch (Berkeley, CA); Mark Reisinger (Berkeley, CA); Ernest Sanders (Berkeley, CA); Richard Broglie (Berkeley, CA); Rosemary Clark (Berkeley, CA); Karsten Temme (Berkeley, CA)
Assignee: Pivot Bio, Inc.
C05C3/00C05C9/005C05F11/08
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12,391,624
App. No.
17/258,024
Filed
Jan 5, 2021
Granted
Aug 19, 2025
Kind
B2
Art Unit
1655
USPC
435/410
Abstract

The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmospheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer, by mitigating or eliminating the need for exogenous nitrogen-containing fertilizers. The remodeled microbes have unique colonization and nitrogen fixation abilities, which enable the microbes to deliver nitrogen to a cereal plant in a spatially targeted (e.g. rhizospheric) and temporally targeted (e.g. during advantageous stages of plants life cycle) manner. The microbes are able to replace the standard agricultural practice of sidedressing and enable a more environmentally sustainable form of farming. The present disclosure also provides methods of using non-intergeneric remodeled microbes, for example, to fix atmospheric nitrogen by reducing or eliminating the need for exogenous nitrogen-containing fertilizers, to increase yield, and to reduce infield variability in the yield.

Claims (60)

1. A method of providing fixed atmospheric nitrogen to a cereal plant, comprising:

a. providing to a locus a plurality of non-intergeneric remodeled bacteria that each produce fixed N of at least about 5.49×10 −13 mmol of N per CFU per hour, wherein the plurality of non-intergeneric remodeled bacteria comprise at least one genetic variation introduced into at least one gene, or non-coding polynucleotide, of the nitrogen fixation or assimilation genetic regulatory network that results in one or more of: increased expression or activity of NifA or glutaminase; decreased expression or activity of NifL, NtrB, glutamine synthetase, GlnB, GlnK, DraT, AmtB; decreased adenylyl-removing activity of GlnE; or decreased uridylyl-removing activity of GlnD; and

b. providing to the locus a plurality of cereal plants,

wherein said plurality of non-intergeneric remodeled bacteria colonize the root surface of said plurality of cereal plants and supply the cereal plants with fixed N, and wherein said plurality of non-intergeneric remodeled bacteria produce in the aggregate at least about 15 pounds of fixed N per acre over the course of at least about 10 days to about 60 days.

2. The method according to claim 1 , wherein the plurality of non-intergeneric remodeled bacteria each produce fixed N of at least about 2.75×10 −12 mmol of N per CFU per hour.

3. The method according to claim 1 , wherein the plurality of non-intergeneric remodeled bacteria colonize the root surface of the plurality of cereal plants at a total aggregate CFU per acre concentration of about 5×10 13 for at least about 20 days.

4. The method according to claim 1 , wherein exogenous nitrogen is not applied to said locus after the plurality of non-intergeneric remodeled bacteria and cereal plants are applied.

5. The method according to claim 1 , wherein said cereal plant is corn, rice, wheat, barley, sorghum , millet, oat, rye, or triticale.

6. The method according to claim 1 , wherein each member of the plurality of non-intergeneric remodeled bacteria comprises at least one genetic variation introduced into at least one gene, or non-coding polynucleotide, involved in a pathway selected from the group consisting of: the nitrogen fixation or assimilation genetic regulatory network, exopolysaccharide production, endo-polygalaturonase production, trehalose production, and glutamine conversion.

7. The method according to claim 1 , wherein each member of the plurality of non-intergeneric remodeled bacteria comprises an introduced control sequence operably linked to at least one gene of the nitrogen fixation or assimilation genetic regulatory network.

8. The method according to claim 1 , wherein each member of the plurality of non-intergeneric remodeled bacteria comprises a heterologous promoter operably linked to at least one gene of the nitrogen fixation or assimilation genetic regulatory network.

9. The method according to claim 1 , wherein each member of the plurality of non-intergeneric remodeled bacteria comprises at least one genetic variation introduced into a member selected from the group consisting of: nifA, nifL, ntrB, ntrC, polynucleotide encoding glutamine synthetase, glnA, glnB, glnK, drat, amtB, polynucleotide encoding glutaminase, glnD, glnE, nifJ, nifH, nifD, nifK, nifY, nifE, nifN, nifU, nifS, nifV, nifW, nifZ, nifM, nifF, nifB, nifQ, a gene associated with biosynthesis of a nitrogenase enzyme, bcsii, bcsiii, yjbE, fhaB, pehA, otsB, treZ, glsA2, and combinations thereof.

10. The method according to claim 1 , wherein each member of the plurality of non-intergeneric remodeled bacteria comprises at least one of: a mutated nifL gene that has been altered to comprise a heterologous promoter inserted into said nifL gene; a mutated glnE gene that results in a truncated GlnE protein lacking an adenylyl-removing (AR) domain; a mutated amtB gene that results in the lack of expression of said amtB gene; and combinations thereof.

11. The method according to claim 1 , wherein the plurality of non-intergeneric remodeled bacteria comprise bacteria selected from: Rahnella aquatilis, Klebsiella variicola, Achromobacter spiritinus, Achromobacter marplatensis, Microbacterium murale, Kluyvera intermedia, Kosakonia pseudosacchari, Enterobacter sp., Azospirillum lipoferum, Kosakonia sacchari , and combinations thereof.

12. The method according to claim 1 , wherein the plurality of non-intergeneric remodeled bacteria comprise bacteria selected from: a bacteria deposited as NCMA 201701002, a bacteria deposited as NCMA 201708004, a bacteria deposited as NCMA 201708003, a bacteria deposited as NCMA 201708002, a bacteria deposited as NCMA 201712001, a bacteria deposited as NCMA 201712002, and combinations thereof.

13. The method according to claim 1 , wherein the plurality of non-intergeneric remodeled bacteria comprise bacteria comprising a nucleic acid sequence that shares at least about 90% sequence identity to a nucleic acid sequence selected from SEQ ID NOs: 177-260, 296-303, and 458-469.

14. A method for increasing corn yield per acre in agriculturally challenging soil, comprising:

a. providing to a locus located in an agriculturally challenging soil a plurality of non-intergeneric remodeled bacteria that each produce fixed N of at least about 5.49×10 −13 mmol of N per CFU per hour; and

b. providing to the locus located in an agriculturally challenging soil a plurality of corn plants,

wherein said plurality of non-intergeneric remodeled bacteria colonize the root surface of said plurality of corn plants and supply the corn plants with fixed N, and

wherein said agriculturally challenging soil comprises a soil that comprises at least about 30% sand, and

wherein said plurality of corn plants achieve at least a 1 bushel per acre yield increase, as compared to a control plurality of corn plants when the control plurality of corn plants is provided to the locus.

15. The method according to claim 14 , wherein said agriculturally challenging soil comprises at least one of: a) at least about 40% sand; b) less than about 30% silt; c) less than about 20% clay; d) a pH of about 5 to 8; and e) an organic matter content of about 0.40 to about 2.8.

16. The method according to claim 14 , wherein said agriculturally challenging soil is a sandy loam or loam soil.

17. The method according to claim 14 , wherein said plurality of corn plants achieve at least a 5 bushel per acre yield increase, as compared to a control plurality of corn plants.

18. The method according to claim 14 , wherein exogenous nitrogen is not applied to said locus after the plurality of non-intergeneric remodeled bacteria and corn plants are supplied.

19. The method according to claim 14 , wherein exogenous nitrogen is not applied to said locus after the plurality of non-intergeneric remodeled bacteria and corn plants are supplied, but wherein exogenous nitrogen is applied to the control plurality of corn plants, said control plurality of corn plants not being provided with a plurality of non-intergeneric remodeled bacteria.

20. The method according to claim 14 , wherein said plurality of non-intergeneric remodeled bacteria produce in the aggregate at least about 15 pounds of fixed N per acre over the course of at least about 10 days to about 60 days.

21. The method according to claim 14 , wherein the plurality of non-intergeneric remodeled bacteria each produce fixed N of at least about 2.75×10 −12 mmol of N per CFU per hour.

22. The method according to claim 14 , wherein the plurality of non-intergeneric remodeled bacteria colonize the root surface of the plurality of corn plants at a total aggregate CFU per acre concentration of about 5×10 13 for at least about 20 days.

23. The method according to claim 14 , wherein each member of the plurality of non-intergeneric remodeled bacteria comprises at least one genetic variation introduced into at least one gene, or non-coding polynucleotide, involved in a pathway selected from the group consisting of: the nitrogen fixation or assimilation genetic regulatory network, exopolysaccharide production, endo-polygalaturonase production, trehalose production, and glutamine conversion.

24. The method according to claim 14 , wherein each member of the plurality of non-intergeneric remodeled bacteria comprises an introduced control sequence operably linked to at least one gene of the nitrogen fixation or assimilation genetic regulatory network.

25. The method according to claim 14 , wherein each member of the plurality of non-intergeneric remodeled bacteria comprises a heterologous promoter operably linked to at least one gene of the nitrogen fixation or assimilation genetic regulatory network.

26. The method according to claim 14 , wherein each member of the plurality of non-intergeneric remodeled bacteria comprises at least one genetic variation introduced into a member selected from the group consisting of: nifA, nifL, ntrB, ntrC, polynucleotide encoding glutamine synthetase, glnA, glnB, glnK, drat, amtB, polynucleotide encoding glutaminase, glnD, glnE, nifJ, nifH, nifD, nifK, nifY, nifE, nifN, nifU, nifS, nifV, nifW, nifZ, nifM, nifF, nifB, nifQ, a gene associated with biosynthesis of a nitrogenase enzyme, bcsii, bcsiii, yjbE, fhaB, pehA, otsB, treZ, glsA2, and combinations thereof.

27. The method according to claim 14 , wherein each member of the plurality of non-intergeneric remodeled bacteria comprises at least one genetic variation introduced into at least one gene, or non-coding polynucleotide, of the nitrogen fixation or assimilation genetic regulatory network that results in one or more of: increased expression or activity of NifA or glutaminase; decreased expression or activity of NifL, NtrB, glutamine synthetase, GlnB, GlnK, DraT, AmtB; decreased adenylyl-removing activity of GlnE; or decreased uridylyl-removing activity of GlnD.

28. The method according to claim 14 , wherein each member of the plurality of non-intergeneric remodeled bacteria comprises at least one of: a mutated nifL gene that has been altered to comprise a heterologous promoter inserted into said nifL gene; a mutated glnE gene that results in a truncated GlnE protein lacking an adenylyl-removing (AR) domain; a mutated amtB gene that results in the lack of expression of said amtB gene; and combinations thereof.

29. The method according to claim 14 , wherein the plurality of non-intergeneric remodeled bacteria comprise bacteria selected from: Rahnella aquatilis, Klebsiella variicola, Achromobacter spiritinus, Achromobacter marplatensis, Microbacterium murale, Kluyvera intermedia, Kosakonia pseudosacchari, Enterobacter sp., Azospirillum lipoferum, Kosakonia sacchari , and combinations thereof.

30. The method according to claim 14 , wherein the plurality of non-intergeneric remodeled bacteria comprise bacteria selected from: a bacteria deposited as NCMA 201701002, a bacteria deposited as NCMA 201708004, a bacteria deposited as NCMA 201708003, a bacteria deposited as NCMA 201708002, a bacteria deposited as NCMA 201712001, a bacteria deposited as NCMA 201712002, and combinations thereof.

31. The method according to claim 14 , wherein the plurality of non-intergeneric remodeled bacteria comprise bacteria with a nucleic acid sequence that shares at least about 90% sequence identity to a nucleic acid sequence selected from SEQ ID NOs: 177-260, 296-303, and 458-469.

32. A method for reducing infield variability for corn yield per acre, comprising:

a. providing to a locus a plurality of non-intergeneric remodeled bacteria that each produce fixed N of at least about 5.49×10 −13 mmol of N per CFU per hour; and

b. providing to the locus a plurality of corn plants,

wherein said plurality of non-intergeneric remodeled bacteria colonize the root surface of said plurality of corn plants and supply the corn plants with fixed N, and

wherein the standard deviation of corn mean yield measured across the locus as measured in bushels per acre is lower for the plurality of corn plants colonized by said non-intergeneric remodeled bacteria, as compared to a control plurality of corn plants when the control plurality of corn plants is provided to the locus.

33. The method according to claim 32 , wherein the standard deviation for the plurality of corn plants colonized by said non-intergeneric remodeled bacteria is less than 19 bushels per acre, as compared to the control plurality of corn plants, said control plurality of corn plants not being colonized by non-intergeneric remodeled bacteria.

34. The method according to claim 32 , wherein the yield between the plurality of corn plants colonized by the non-intergeneric remodeled bacteria is within 1-10% of the yield of the control plurality of corn plants, said control plurality of corn plants not being colonized by non-intergeneric remodeled bacteria.

35. The method according to claim 32 , wherein exogenous nitrogen is not applied to said locus after the plurality of non-intergeneric remodeled bacteria and corn plants are supplied, but wherein exogenous nitrogen is applied to the control plurality of corn plants, said control plurality of corn plants not being provided with a plurality of non-intergeneric remodeled bacteria.

36. The method according to claim 32 , wherein said plurality of non-intergeneric remodeled bacteria produce in the aggregate at least about 15 pounds of fixed N per acre over the course of at least about 10 days to about 60 days.

37. The method according to claim 32 , wherein exogenous nitrogen is not applied as a sidedressing.

38. The method according to claim 32 , wherein the plurality of non-intergeneric remodeled bacteria each produce fixed N of at least about 2.75×10 −12 mmol of N per CFU per hour.

39. The method according to claim 32 , wherein the plurality of non-intergeneric remodeled bacteria colonize the root surface of the plurality of corn plants at a total aggregate CFU per acre concentration of about 5×10 13 for at least about 20 days.

40. The method according to claim 32 , wherein each member of the plurality of non-intergeneric remodeled bacteria comprises at least one genetic variation introduced into at least one gene, or non-coding polynucleotide, involved in a pathway selected from the group consisting of: the nitrogen fixation or assimilation genetic regulatory network, exopolysaccharide production, endo-polygalaturonase production, trehalose production, and glutamine conversion.

41. The method according to claim 32 , wherein each member of the plurality of non-intergeneric remodeled bacteria comprises an introduced control sequence operably linked to at least one gene of the nitrogen fixation or assimilation genetic regulatory network.

42. The method according to claim 32 , wherein each member of the plurality of non-intergeneric remodeled bacteria comprises a heterologous promoter operably linked to at least one gene of the nitrogen fixation or assimilation genetic regulatory network.

43. The method according to claim 32 , wherein each member of the plurality of non-intergeneric remodeled bacteria comprises at least one genetic variation introduced into a member selected from the group consisting of: nifA, nifL, ntrB, ntrC, polynucleotide encoding glutamine synthetase, glnA, glnB, glnK, drat, amtB, polynucleotide encoding glutaminase, glnD, glnE, nifJ, nifH, nifD, nifK, nifY, nifE, nifN, nifU, nifS, nifV, nifW, nifZ, nifM, nifF, nifB, nifQ, a gene associated with biosynthesis of a nitrogenase enzyme, bcsii, bcsiii, yjbE, fhaB, pehA, otsB, treZ, glsA2, and combinations thereof.

44. The method according to claim 32 , wherein each member of the plurality of non-intergeneric remodeled bacteria comprises at least one genetic variation introduced into at least one gene, or non-coding polynucleotide, of the nitrogen fixation or assimilation genetic regulatory network that results in one or more of: increased expression or activity of NifA or glutaminase; decreased expression or activity of NifL, NtrB, glutamine synthetase, GlnB, GlnK, DraT, AmtB; decreased adenylyl-removing activity of GlnE; or decreased uridylyl-removing activity of GlnD.

45. The method according to claim 32 , wherein each member of the plurality of non-intergeneric remodeled bacteria comprises at least one of: a mutated nifL gene that has been altered to comprise a heterologous promoter inserted into said nifL gene; a mutated glnE gene that results in a truncated GlnE protein lacking an adenylyl-removing (AR) domain; a mutated amtB gene that results in the lack of expression of said amtB gene; and combinations thereof.

46. The method according to claim 32 , wherein the plurality of non-intergeneric remodeled bacteria comprise bacteria selected from: Rahnella aquatilis, Klebsiella variicola, Achromobacter spiritinus, Achromobacter marplatensis, Microbacterium murale, Kluyvera intermedia, Kosakonia pseudosacchari, Enterobacter sp., Azospirillum lipoferum, Kosakonia sacchari , and combinations thereof.

47. The method according to claim 32 , wherein the plurality of non-intergeneric remodeled bacteria comprise bacteria selected from: a bacteria deposited as NCMA 201701002, a bacteria deposited as NCMA 201708004, a bacteria deposited as NCMA 201708003, a bacteria deposited as NCMA 201708002, a bacteria deposited as NCMA 201712001, a bacteria deposited as NCMA 201712002, and combinations thereof.

48. The method according to claim 32 , wherein the plurality of non-intergeneric remodeled bacteria comprise bacteria with a nucleic acid sequence that shares at least about 90% sequence identity to a nucleic acid sequence selected from SEQ ID NOs: 177-260, 296-303, and 458-469.

Assignments (2)
SECURITY INTEREST Recorded Dec 22, 2025
From: PIVOT BIO, INC.
To: HSBC BANK USA, N.A.
Reel/Frame 073285/0020 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 29, 2021
From: TAMSIR, ALVIN; BLOCH, SARAH; REISINGER, MARK; SANDERS, ERNEST; BROGLIE, RICHARD; CLARK, ROSEMARY; TEMME, KARSTEN
To: PIVOT BIO, INC.
Reel/Frame 058227/0702 →
Continuity (3)
Provisional Application 62801504 · Feb 5, 2019
Provisional Application 62696452 · Jul 11, 2018
Related Publication 20220396530A1 · Dec 15, 2022
References Cited (400)
US 1520545A · Murphy · 1924 [cited by applicant]
US 4782022A · Puhler et al. · 1988 [cited by applicant]
US 4832728A · Allan et al. · 1989 [cited by applicant]
US 5071743A · Slilaty et al. · 1991 [cited by applicant]
US 5188960A · Payne et al. · 1993 [cited by applicant]
US 5229291A · Nielsen et al. · 1993 [cited by applicant]
US 5354670A · Nickoloff et al. · 1994 [cited by applicant]
US 5427785A · Ronson et al. · 1995 [cited by applicant]
US 5780270A · Lesley · 1998 [cited by applicant]
US 5789166A · Bauer et al. · 1998 [cited by applicant]
US 5877012A · Estruch et al. · 1999 [cited by applicant]
US 5880275A · Fischhoff et al. · 1999 [cited by applicant]
US 5916029A · Smith et al. · 1999 [cited by applicant]
US 6033861A · Schafer et al. · 2000 [cited by applicant]
US 6033874A · Baum et al. · 2000 [cited by applicant]
US 6083499A · Narva et al. · 2000 [cited by applicant]
US 6107279A · Estruch et al. · 2000 [cited by applicant]
US 6114148A · Seed et al. · 2000 [cited by applicant]
US 6127180A · Narva et al. · 2000 [cited by applicant]
US 6137033A · Estruch et al. · 2000 [cited by applicant]
US 6218188B1 · Cardineau et al. · 2001 [cited by applicant]
US 6248535B1 · Danenberg et al. · 2001 [cited by applicant]
US 6326351B1 · Donovan et al. · 2001 [cited by applicant]
US 6340593B1 · Cardineau et al. · 2002 [cited by applicant]
US 6391548B1 · Bauer et al. · 2002 [cited by applicant]
US 6399330B1 · Donovan et al. · 2002 [cited by applicant]
US 6548289B1 · Beynon et al. · 2003 [cited by applicant]
US 6548291B1 · Narva et al. · 2003 [cited by applicant]
US 6596509B1 · Bauer et al. · 2003 [cited by applicant]
US 6624145B1 · Narva et al. · 2003 [cited by applicant]
US 6673610B2 · Miyawaki et al. · 2004 [cited by applicant]
US 6713063B1 · Malvar et al. · 2004 [cited by applicant]
US 6713285B2 · Bauer et al. · 2004 [cited by applicant]
US 6773900B2 · Short et al. · 2004 [cited by applicant]
US 6841358B1 · Locht et al. · 2005 [cited by applicant]
US 6949626B2 · Donovan et al. · 2005 [cited by applicant]
US 6962705B2 · Malvar et al. · 2005 [cited by applicant]
US 7064249B2 · Corbin et al. · 2006 [cited by applicant]
US 7070982B2 · Malvar et al. · 2006 [cited by applicant]
US 7084331B2 · Isawa et al. · 2006 [cited by applicant]
US 7105332B2 · Abad et al. · 2006 [cited by applicant]
US 7132265B2 · Bauer et al. · 2006 [cited by applicant]
US 7244820B2 · Miles et al. · 2007 [cited by applicant]
US 7329736B2 · Abad et al. · 2008 [cited by applicant]
US 7374753B1 · Farmer · 2008 [cited by examiner]
US 7378499B2 · Abad et al. · 2008 [cited by applicant]
US 7385107B2 · Donovan et al. · 2008 [cited by applicant]
US 7419698B2 · Jones · 2008 [cited by applicant]
US 7438901B2 · Loessner · 2008 [cited by examiner]
US 7449552B2 · Abad et al. · 2008 [cited by applicant]
US 7462760B2 · Abad et al. · 2008 [cited by applicant]
US 7470427B2 · Cocking · 2008 [cited by applicant]
US 7476781B2 · Abad et al. · 2009 [cited by applicant]
US 7485451B2 · Vandergheynst et al. · 2009 [cited by applicant]
US 7491698B2 · Hey et al. · 2009 [cited by applicant]
US 7491869B2 · Abad et al. · 2009 [cited by applicant]
US 7504229B2 · Donovan et al. · 2009 [cited by applicant]
US 7615686B2 · Miles et al. · 2009 [cited by applicant]
US 7803943B2 · Mao et al. · 2010 [cited by applicant]
US 7858849B2 · Cerf et al. · 2010 [cited by applicant]
US 7923602B2 · Carozzi et al. · 2011 [cited by applicant]
US 8076142B2 · Huang et al. · 2011 [cited by applicant]
US 8084416B2 · Sampson et al. · 2011 [cited by applicant]
US 8084418B2 · Hey et al. · 2011 [cited by applicant]
US 8137665B2 · Cocking · 2012 [cited by applicant]
US 8236757B2 · Carozzi et al. · 2012 [cited by applicant]
US 8237020B2 · Miles et al. · 2012 [cited by applicant]
US 8268584B1 · Harwood et al. · 2012 [cited by applicant]
US 8304604B2 · Lira et al. · 2012 [cited by applicant]
US 8304605B2 · Lira et al. · 2012 [cited by applicant]
US 8318900B2 · Sampson et al. · 2012 [cited by applicant]
US 8319019B2 · Abad et al. · 2012 [cited by applicant]
US 8334366B1 · Hughes et al. · 2012 [cited by applicant]
US 8334431B2 · Sampson et al. · 2012 [cited by applicant]
US 8377671B2 · Cournac et al. · 2013 [cited by applicant]
US 8481026B1 · Woodruff et al. · 2013 [cited by applicant]
US 8513494B2 · Wu et al. · 2013 [cited by applicant]
US 8530411B2 · Cerf et al. · 2013 [cited by applicant]
US 8575433B2 · Cerf et al. · 2013 [cited by applicant]
US 8686233B2 · Cerf et al. · 2014 [cited by applicant]
US 8759619B2 · Sampson et al. · 2014 [cited by applicant]
US 8795965B2 · Zhang · 2014 [cited by applicant]
US 8802933B2 · Abad et al. · 2014 [cited by applicant]
US 8802934B2 · Abad et al. · 2014 [cited by applicant]
US 9150851B2 · Wigley et al. · 2015 [cited by applicant]
US 9321697B2 · Das et al. · 2016 [cited by applicant]
US 9487451B2 · Doty et al. · 2016 [cited by applicant]
US 9512431B2 · Mirsky et al. · 2016 [cited by applicant]
US 9657298B2 · Soto, Sr. et al. · 2017 [cited by applicant]
US 9957509B2 · Mirsky et al. · 2018 [cited by applicant]
US 9975817B2 · Temme et al. · 2018 [cited by applicant]
US 10384983B2 · Temme et al. · 2019 [cited by applicant]
US 10556839B2 · Temme et al. · 2020 [cited by applicant]
US 10662432B2 · Mirsky et al. · 2020 [cited by applicant]
US 10919814B2 · Temme et al. · 2021 [cited by applicant]
US 10934226B2 · Temme et al. · 2021 [cited by applicant]
US 10968446B2 · Zhao et al. · 2021 [cited by applicant]
US 11479516B2 · Voigt et al. · 2022 [cited by applicant]
US 11565979B2 · Temme et al. · 2023 [cited by applicant]
US 11678667B2 · Reisinger et al. · 2023 [cited by applicant]
US 11678668B2 · Reisinger et al. · 2023 [cited by applicant]
US 11739032B2 · Temme et al. · 2023 [cited by applicant]
US 11946162B2 · Zhao et al. · 2024 [cited by applicant]
US 11963530B2 · Reisinger et al. · 2024 [cited by applicant]
US 11993778B2 · Tamsir et al. · 2024 [cited by applicant]
US 12151988B2 · Tamsir et al. · 2024 [cited by applicant]
US 20040197916A1 · Carozzi et al. · 2004 [cited by applicant]
US 20040197917A1 · Carozzi et al. · 2004 [cited by applicant]
US 20040210964A1 · Carozzi et al. · 2004 [cited by applicant]
US 20040210965A1 · Carozzi et al. · 2004 [cited by applicant]
US 20040216186A1 · Carozzi et al. · 2004 [cited by applicant]
US 20040235663A1 · Cocking · 2004 [cited by applicant]
US 20040241847A1 · Okuyama et al. · 2004 [cited by applicant]
US 20040250311A1 · Carozzi et al. · 2004 [cited by applicant]
US 20050081262A1 · Cook et al. · 2005 [cited by applicant]
US 20050266541A1 · Dillon · 2005 [cited by applicant]
US 20060112447A1 · Bogdanova et al. · 2006 [cited by applicant]
US 20060127988A1 · Wood et al. · 2006 [cited by applicant]
US 20060191034A1 · Baum et al. · 2006 [cited by applicant]
US 20060243011A1 · Someus · 2006 [cited by applicant]
US 20080295207A1 · Baum et al. · 2008 [cited by applicant]
US 20080311632A1 · Figge et al. · 2008 [cited by applicant]
US 20090105076A1 · Stewart et al. · 2009 [cited by applicant]
US 20090137390A1 · Triplett · 2009 [cited by applicant]
US 20090144852A1 · Tomso et al. · 2009 [cited by applicant]
US 20090162477A1 · Nadel et al. · 2009 [cited by applicant]
US 20090258404A1 · Mikkelsen et al. · 2009 [cited by applicant]
US 20090308121A1 · Reddy et al. · 2009 [cited by applicant]
US 20100005543A1 · Sampson et al. · 2010 [cited by applicant]
US 20100017914A1 · Hart et al. · 2010 [cited by applicant]
US 20100028870A1 · Welch et al. · 2010 [cited by applicant]
US 20100197592A1 · Heinrichs · 2010 [cited by applicant]
US 20100267147A1 · Qiao · 2010 [cited by applicant]
US 20100298211A1 · Carozzi et al. · 2010 [cited by applicant]
US 20110023184A1 · Desai et al. · 2011 [cited by applicant]
US 20110064710A1 · Benson et al. · 2011 [cited by applicant]
US 20110104690A1 · Yu et al. · 2011 [cited by applicant]
US 20110263488A1 · Carozzi et al. · 2011 [cited by applicant]
US 20120015806A1 · Paikray et al. · 2012 [cited by applicant]
US 20120107889A1 · Doty et al. · 2012 [cited by applicant]
US 20120192605A1 · McSpadden Gardener et al. · 2012 [cited by applicant]
US 20120266332A1 · Kuykendall · 2012 [cited by applicant]
US 20120278954A1 · Bowen et al. · 2012 [cited by applicant]
US 20120284813A1 · Olivier et al. · 2012 [cited by applicant]
US 20120311745A1 · Meade et al. · 2012 [cited by applicant]
US 20120311746A1 · Meade et al. · 2012 [cited by applicant]
US 20120317681A1 · Meade et al. · 2012 [cited by applicant]
US 20120317682A1 · Meade et al. · 2012 [cited by applicant]
US 20120324605A1 · Meade et al. · 2012 [cited by applicant]
US 20120324606A1 · Meade et al. · 2012 [cited by applicant]
US 20120331589A1 · Meade et al. · 2012 [cited by applicant]
US 20120331590A1 · Meade et al. · 2012 [cited by applicant]
US 20130116170A1 · Graser et al. · 2013 [cited by applicant]
US 20130126428A1 · Jones et al. · 2013 [cited by applicant]
US 20130167268A1 · Narva et al. · 2013 [cited by applicant]
US 20130167269A1 · Narva et al. · 2013 [cited by applicant]
US 20140011261A1 · Wang et al. · 2014 [cited by applicant]
US 20140155283A1 · Venkateswaran et al. · 2014 [cited by applicant]
US 20140182018A1 · Lang et al. · 2014 [cited by applicant]
US 20140223598A1 · Sampson et al. · 2014 [cited by applicant]
US 20140223599A1 · Sampson et al. · 2014 [cited by applicant]
US 20140230504A1 · Finlayson et al. · 2014 [cited by applicant]
US 20140273226A1 · Wu · 2014 [cited by applicant]
US 20140301990A1 · Gregory et al. · 2014 [cited by applicant]
US 20140329326A1 · Mirsky et al. · 2014 [cited by applicant]
US 20140336050A1 · Soto, Sr. et al. · 2014 [cited by applicant]
US 20150080261A1 · Wigley et al. · 2015 [cited by applicant]
US 20150101373A1 · Munusamy et al. · 2015 [cited by applicant]
US 20150128670A1 · Das · 2015 [cited by applicant]
US 20150237807A1 · Valiquette · 2015 [cited by applicant]
US 20150239789A1 · Kang et al. · 2015 [cited by applicant]
US 20150315570A1 · Zhao et al. · 2015 [cited by applicant]
US 20160174570A1 · Vujanovic et al. · 2016 [cited by applicant]
US 20160264929A1 · Barney et al. · 2016 [cited by applicant]
US 20160292355A1 · Lou et al. · 2016 [cited by applicant]
US 20160295868A1 · Jones et al. · 2016 [cited by applicant]
US 20170016013A1 · Fu et al. · 2017 [cited by applicant]
US 20170152519A1 · Mirsky et al. · 2017 [cited by applicant]
US 20170367349A1 · Gruver et al. · 2017 [cited by applicant]
US 20180020671A1 · Wigley et al. · 2018 [cited by applicant]
US 20180060771A1 · Mangin · 2018 [cited by applicant]
US 20180065896A1 · Van Iersel et al. · 2018 [cited by applicant]
US 20180073028A1 · Mirsky et al. · 2018 [cited by applicant]
US 20180290942A1 · Voigt et al. · 2018 [cited by applicant]
US 20190039964A1 · Temme et al. · 2019 [cited by applicant]
US 20190144352A1 · Temme et al. · 2019 [cited by applicant]
US 20190185871A1 · Zhou et al. · 2019 [cited by applicant]
US 20190325534A1 · Perry et al. · 2019 [cited by applicant]
US 20190339964A1 · Young et al. · 2019 [cited by applicant]
US 20200115715A1 · Mirsky et al. · 2020 [cited by applicant]
US 20200299637A1 · Voigt et al. · 2020 [cited by applicant]
US 20200308594A1 · Tamsir et al. · 2020 [cited by applicant]
US 20200331820A1 · Tamsir et al. · 2020 [cited by applicant]
US 20210009483A1 · Temme et al. · 2021 [cited by applicant]
US 20210163374A1 · Bloch et al. · 2021 [cited by applicant]
US 20210214282A1 · Temme et al. · 2021 [cited by applicant]
US 20210284995A1 · Zhao et al. · 2021 [cited by applicant]
US 20210315212A1 · Rezaei et al. · 2021 [cited by applicant]
US 20210345618A1 · Bloch et al. · 2021 [cited by applicant]
US 20220017911A1 · Temme et al. · 2022 [cited by applicant]
US 20220079163A1 · Reisinger et al. · 2022 [cited by applicant]
US 20220090095A1 · Higgins et al. · 2022 [cited by applicant]
US 20220106238A1 · Rezaei et al. · 2022 [cited by applicant]
US 20220127627A1 · Bloch et al. · 2022 [cited by applicant]
US 20220162544A1 · Voigt et al. · 2022 [cited by applicant]
US 20220211048A1 · Temme et al. · 2022 [cited by applicant]
US 20220282340A1 · Ryu et al. · 2022 [cited by applicant]
US 20220411344A1 · Voigt et al. · 2022 [cited by applicant]
US 20230019267A1 · Hapes et al. · 2023 [cited by applicant]
US 20230033451A1 · Reisinger · 2023 [cited by examiner]
US 20230062568A1 · Temme et al. · 2023 [cited by applicant]
US 20230148607A1 · Rezaei et al. · 2023 [cited by applicant]
US 20230175959A1 · Wood et al. · 2023 [cited by applicant]
US 20230257317A1 · Temme et al. · 2023 [cited by applicant]
US 20230276807A1 · Reisinger et al. · 2023 [cited by applicant]
US 20230295559A1 · Ozaydin Eskiyenenturk et al. · 2023 [cited by applicant]
US 20240010576A1 · Temme et al. · 2024 [cited by applicant]
US 20240196903A1 · Reisinger et al. · 2024 [cited by applicant]
US 20240294953A1 · Eskiyenenturk et al. · 2024 [cited by applicant]
US 20240298647A1 · Reisinger et al. · 2024 [cited by applicant]
US 20240327851A1 · Tamsir et al. · 2024 [cited by applicant]
US 20240397955A1 · Belcher et al. · 2024 [cited by applicant]
BR 112020026676A2 · 2021 [cited by applicant]
CA 2051071A1 · 1993 [cited by applicant]
CN 101774854A · 2010 [cited by applicant]
CN 101899430A · 2010 [cited by applicant]
CN 102747002A · 2012 [cited by applicant]
CN 102770543A · 2012 [cited by applicant]
CN 103183572A · 2013 [cited by applicant]
CN 106086042A · 2016 [cited by applicant]
CN 108027849A · 2018 [cited by applicant]
CN 108271339A · 2018 [cited by applicant]
CN 108602729A · 2018 [cited by applicant]
FR 2910230A1 · 2008 [cited by applicant]
JP 2018507708A · 2018 [cited by applicant]
RU 2511299C2 · 2014 [cited by applicant]
RU 2588473C1 · 2016 [cited by applicant]
WO WO8704182A1 · 1987 [cited by applicant]
WO WO9305154A1 · 1993 [cited by applicant]
WO WO9320685A1 · 1993 [cited by applicant]
WO WO9801088A1 · 1998 [cited by applicant]
WO WO9909834A2 · 1999 [cited by applicant]
WO WO0057183A1 · 2000 [cited by applicant]
WO WO0107567A1 · 2001 [cited by applicant]
WO WO2004074462A2 · 2004 [cited by applicant]
WO WO2005021585A2 · 2005 [cited by applicant]
WO WO2005038032A1 · 2005 [cited by applicant]
WO WO2006005100A1 · 2006 [cited by applicant]
WO WO2006083891A2 · 2006 [cited by applicant]
WO WO2006119457A1 · 2006 [cited by applicant]
WO WO2007027776A2 · 2007 [cited by applicant]
WO WO2009060012A2 · 2009 [cited by applicant]
WO WO2009091557A1 · 2009 [cited by applicant]
WO WO2011099019A1 · 2011 [cited by applicant]
WO WO2011099024A1 · 2011 [cited by applicant]
WO WO2011103247A2 · 2011 [cited by applicant]
WO WO2011103248A2 · 2011 [cited by applicant]
WO WO2011154960A1 · 2011 [cited by applicant]
WO WO2012139004A2 · 2012 [cited by applicant]
WO WO2012174271A2 · 2012 [cited by applicant]
WO WO2013076687A2 · 2013 [cited by applicant]
WO WO2013090628A1 · 2013 [cited by applicant]
WO WO2013132518A1 · 2013 [cited by applicant]
WO WO2013148290A1 · 2013 [cited by applicant]
WO WO2014042517A2 · 2014 [cited by applicant]
WO WO2014071182A1 · 2014 [cited by applicant]
WO WO2014078647A1 · 2014 [cited by applicant]
WO WO2014121366A1 · 2014 [cited by applicant]
WO WO2014201044A2 · 2014 [cited by applicant]
WO WO2016016629A1 · 2016 [cited by applicant]
WO WO2016016630A1 · 2016 [cited by applicant]
WO WO2016100727A1 · 2016 [cited by applicant]
WO WO2016108976A1 · 2016 [cited by applicant]
WO WO2016146955A1 · 2016 [cited by applicant]
WO WO2016178580A2 · 2016 [cited by applicant]
WO WO2016179046A1 · 2016 [cited by applicant]
WO WO2016181228A2 · 2016 [cited by applicant]
WO WO2016191828A1 · 2016 [cited by applicant]
WO WO2016200987A1 · 2016 [cited by applicant]
WO WO2017011602A1 · 2017 [cited by applicant]
WO WO2017042833A1 · 2017 [cited by applicant]
WO WO2017062412A1 · 2017 [cited by applicant]
WO WO2017069717A1 · 2017 [cited by applicant]
WO WO2018132774A1 · 2018 [cited by applicant]
WO WO2019032926A1 · 2019 [cited by applicant]
WO WO2019084059A2 · 2019 [cited by applicant]
WO WO2019084342A1 · 2019 [cited by applicant]
WO WO2019140125A1 · 2019 [cited by applicant]
WO WO2020006064A2 · 2020 [cited by applicant]
WO WO2020006246A1 · 2020 [cited by applicant]
WO WO2020014498A1 · 2020 [cited by applicant]
WO WO2020061363A1 · 2020 [cited by applicant]
WO WO2020092940A1 · 2020 [cited by applicant]
WO WO2020118111A1 · 2020 [cited by applicant]
WO WO2020132632A2 · 2020 [cited by applicant]
WO WO2020146372A1 · 2020 [cited by applicant]
WO WO2020163251A1 · 2020 [cited by applicant]
WO WO2020190363A1 · 2020 [cited by applicant]
WO WO2020191201A1 · 2020 [cited by applicant]
WO WO2020219893A2 · 2020 [cited by applicant]
WO WO2020219932A1 · 2020 [cited by applicant]
WO WO2021113352A1 · 2021 [cited by applicant]
WO WO2021146209A1 · 2021 [cited by applicant]
WO WO2021221689A1 · 2021 [cited by applicant]
WO WO2021221690A1 · 2021 [cited by applicant]
WO WO2021222567A2 · 2021 [cited by applicant]
WO WO2021222643A1 · 2021 [cited by applicant]
WO WO2021231449A2 · 2021 [cited by applicant]
WO WO2022029661A1 · 2022 [cited by applicant]
WO WO2022029661A9 · 2022 [cited by applicant]
WO WO2022140656A1 · 2022 [cited by applicant]
WO WO2022260676A1 · 2022 [cited by applicant]
WO WO2022261433A1 · 2022 [cited by applicant]
WO WO2023278804A1 · 2023 [cited by applicant]
WO WO2023147050A1 · 2023 [cited by applicant]
WO WO2023154805A2 · 2023 [cited by applicant]
WO WO2024006524A1 · 2024 [cited by applicant]
WO WO2024015230A1 · 2024 [cited by applicant]
WO WO2024137259A1 · 2024 [cited by applicant]
US 8,476,226 B2, 07/2013, Lira et al. (withdrawn) [cited by applicant]
Bali, et al., Excretion of Ammonium by a nifL Mutant of Azotobacter vinelandii fixing Nitrogen. Applied and Environmental Microbiology, May 1992, p. 1711-1718. [cited by applicant]
Barney et al., Gene deletions resulting in increased nitrogen release by azotobacter vinelandii: application of a novel nitrogen biosensor. Appl. Environ. Microbiol. 2015; 81(13):4316-4328. Published online Apr. 17, 201… [cited by applicant]
Baum, et al. “Control of coleopteran insect pests through RNA interference.” Nature Biotechnology (2007); 25(11): 1322-1326. Epub Nov. 4, 2007. [cited by applicant]
Blanco, et al. Sequence and molecular analysis of the nifL gene of Azotobacter vinelandii. Mol Microbiol. Aug. 1993;9(4):869-79. [cited by applicant]
Bosmans, Frank, et al. “Sea anemone venom as a source of insecticidal peptides acting on voltage-gated Na+ channels”, Toxicon; 49(4):550-560, 15 pages (Mar. 2007). [cited by applicant]
Butzen, S., “Corn Seeding Rate Considerations,” Mar. 2019, 4 pages; www.pioneer.com/home/site/us/agronomy/library/corn-seeding-rate-considerations/. [cited by applicant]
Cera. “GM Crop Database. Center for Environmental Risk Assessment (CERA)”, ILSI Research Foundation, at cera-gmc.org/index.php?action=gm_crop_database, 1 page (2010). [cited by applicant]
Chakroun et al., “Bacterial Vegetative Insecticidal Proteins (Vip) from Entomopathogenic Bacteria”, Microbiology and Molecular Biology Reviews; 80(2):329-350 (Mar. 2, 2016). [cited by applicant]
Chiang, et al., “Mutagenic Oligonucleotide-directed PCR Amplification (Mod-PCR): An Efficient Method for Generating Random Base Substitution Mutations in a DNA sequence element,” PCT methods and applications, 1993; 2:21… [cited by applicant]
Ciampitti, I. A., et al., “Physiological perspectives of changes over time in maize yield dependency on nitrogen uptake and associated nitrogen efficiences: A review,” Field Crops Research 133, pp. 48-67, doi:10.1016/j.… [cited by applicant]
Compant, et al., 2010, Plant growth-promoting bacteria in the rhizo- and endosphere of plants: Their role, colonization mechanisms involved and prospects for utilization, Soil Biology and Biochemistry, 42(5), 669-678, d… [cited by applicant]
Crickmore, et al., “Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins”. Microbiol Mol Biol Rev. (Sep. 1998); 62(3): 807-813. [cited by applicant]
Crickmore, N. et al., “Bacillus thuringiensis toxin nomenclature,” Web page, https://web.archive.org/web/20161110143346/http://www.btnomenclature.info/, 2016, 1 page, retrieved from internet on Nov. 30, 2022. [cited by applicant]
Crickmore, N., The VIP nomenclature, http://www.lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/vip.html, retireved from the internet Nov. 11, 2022 (4 total pages). [cited by applicant]
Danhorn, T., et al., 2007, “Biofilm formation by plant-associated bacteria,” Annual Review of Microbiology, 61, 401-422, doi.org/10.1146/annurev.micro.61.080706.093316. [cited by applicant]
Dixon, R. and Kahn, D., “Genetic regulation of biological nitrogen fixation,” Nature Reviews 2004; 2: 621-631. [cited by applicant]
Extended European Search Report, dated Mar. 14, 2022, for European Application No. 19833252.0 (6 total pages). [cited by applicant]
Partial supplementary European Search Report and Search Opinion, dated Nov. 10, 2022, for European Application No. 20751885.3 (16 total pages). [cited by applicant]
Eyraud, Vanessa, et al. “Expression and biological activity of the cystine knot bioinsecticide PA1b (Pea Albumin 1 Subunit b).” PloS ONE, 8(12):e81619, 9 pages (Dec. 2013). [cited by applicant]
First Examination Report, dated Sep. 6, 2022, for Indian Patent Application No. 202117002885 (5 total pages). [cited by applicant]
First Office Action, issued Jun. 22, 2022, for Chinese Patent Application No. 201980053956.9 (English translation) (21 total pages). [cited by applicant]
First Office Action, issued May 27, 2022, for Chinese Patent Application No. 202080027155.8 (English translation) (16 total pages). [cited by applicant]
Gaby et al., “A comprehensive aligned nifH gene database: a multipurpose tool for studies of nitrogen-fixing bacteria,” Database, vol. 2014, Article ID bau001, doi: 10.1093/databse/bau001, 8 pages (Feb. 2014). [cited by applicant]
Glick, B. R. et al., “Plant Growth-Promoting Bacteria: Mechanisms and Applications,” Scientifica, vol. 2012, ID 963401, http://doi.org/10.6064/2012/963401, 15 pages (Oct. 2012). [cited by applicant]
Hidaka, et al. Promotion of the Growth of Rice by Inoculation of Nitrogen-Fixing-Activity-Enhanced Bacteria to the Rhizosphere. In Nitrogen Fixation: From Molecules to Crop Productivity (Part of the Current Plant Scienc… [cited by applicant]
Hoeschle-Zeledon, I. et al., “Regulatory challenges for biological control,” The CGIAR Systemwide Program on Integrated Pest Management, Jan. 2013, SP-IPM Secretariat, International Institute of Tropical Agriculture (II… [cited by applicant]
International Search Report and Written Opinion for Patent Application No. PCT/IB2021/057171, mailed Nov. 5, 2021, 13 pages. [cited by applicant]
International Search Report and Written Opinion, mailed Dec. 3, 2019, for International Application No. PCT/US2019/041429 (19 total pages). [cited by applicant]
International Search Report and Written Opinion, mailed Jun. 25, 2020, for International Application No. PCT/US2020/016471 (18 total pages). [cited by applicant]
Invitation to Pay Additional Fees, and Where Applicable, Protest Fee, dated Apr. 30, 2020, for International Application No. PCT/US2020/016471 (3 total pages). [cited by applicant]
Invitation to Pay Additional Fees, and Where Applicable, Protest Fee, dated Oct. 7, 2019, for International Application No. PCT/US2019/041429 (4 total pages). [cited by applicant]
Ji, K., et al., “Bacterial cellulose synthesis mechanism of facultative anaerobe [cited by applicant]
Kabaluk, J. Todd, et al., eds. The use and regulation of microbial pesticides in representative jurisdictions worldwide. International Organization for Biological Control of Noxious Animals and Plants (IOBC); pp. 1-99 (… [cited by applicant]
Kant, et al., Understanding plant response to nitrogen limitation for the improvement of crop nitrogen use efficiency. Journal of Experimental Botany, 2011; vol. 62, No. 4, pp. 1499-1509. [cited by applicant]
Kim, et al. Constitutive expression of nitrogenase system in Klebsiella oxytoca by gene targeting mutation to the chromosomal nifLA operon. Journal of Biotechnology. vol. 10, Issues 3-4, Jun. 1989, pp. 293-301. [cited by applicant]
King, Glenn F., et al. “Spider-venom peptides: structure, pharmacology, and potential for control of insect pests”, Annual Review of Entomology; 58:475-496 (2013). [cited by applicant]
Kiss, S., et al., “Effect of Urease Inhibitors on Other Enyme Activities, Microbial Counts and Biomass as well as on Respiration and Other Microbial Processes in Soils,” Chapter 8, Improving Efficiency of Urea Fertilize… [cited by applicant]
Lin, Ping, et al. “PC, a novel oral insecticidal toxin from Bacillus bombysepticus involved in host lethality via APN and BtR-175”, Scientific Reports; 5(1): Article 11101 pp. 1-14 (Jun. 9, 2015). [cited by applicant]
Mabrouk, Y. et al., “Chapter 6: Potential of Rhizobia in Improving Nitrogen Flixation and Yields of Legumes,” Symbiosis, IntechOpen, pp. 1-15, (May 2018) https://www.intechopen.com/books/symbiosis/potential-of-rhizobia-… [cited by applicant]
Mao, et al. “Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol.” Nature Biotechnology (2007); 25(11): 1307-1313. Epub Nov. 4, 2007. [cited by applicant]
Marroqui et al. “Enhanced Symbiotic Performance by Rhizobium tropici Glycogen Synthase 17, 18 Mutants,” Journal of Bacteriology, Feb. 1, 2001, vol. 183, No. 3, pp. 854-864. [cited by applicant]
Martinelli, Anne HS, et al. “Structure-function studies on jaburetox, a recombinant insecticidal peptide derived from jack bean ( [cited by applicant]
Marx, et al. Broad-host-range ere-lox system for antibiotic marker recycling in gram-negative bacteria. Biotechniques. Nov. 2002;33(5):1062-1067. [cited by applicant]
Mengel, D., Roots, growth and nutrient uptake. Dept. of Agronomy publication #AGRY-95-08, pp. 1-8 (Rev. May 1995). [cited by applicant]
Naimov, et al., “Solubilization, Activation, and Insecticidal Activity of Bacillus thuringiensis Serovar thompsoni HD542 Crystal Proteins”. Applied and Environmental Microbiology (Dec. 2008); 74(23): 7145-7151. [cited by applicant]
Nam, H. S., et al., 2006, “The dctA gene of Pseudomonas chlororaphisO6 is under RpoN control and is required for effective root colonization and induction of systemic resistance,” FEMS Microbiology Letters, 256(1), 98-1… [cited by applicant]
Nestmann, E.R., Mutagenesis by nitrosoguanidine, ethyl methanesulfonate, and mutator gene mutH in continuous cultures of [cited by applicant]
Office Action for Indonesian Application No. IDP00202100176, dated Oct. 10, 2022, 4 pages. [cited by applicant]
Ortiz-Marquez, J. C. et al., 2014, “Metabolic engineering of ammonium release for nitrogen-fixing multispecies microbial cell-factories,” Metabolic Engineering, 23, pp. 154-164, doi.org/10.1016/j.ymben.2014.03.002. [cited by applicant]
Parker and Feil, “Pore-forming protein toxins: from structure to function”. Prog Biophys Mol Biol. (May 2005); 88(1): 91-142. [cited by applicant]
Purcell, et al., “Cholesterol oxidase: a potent insecticidal protein active against boll weevil larvae”. Biochem Biophys Res Commun. (Nov. 15, 1993); 196(3):1406-1413. [cited by applicant]
Qaim and Zilberman, “Yield Effects of Genetically Modified Crops in Developing Countries”, Science (Feb. 7, 2003); 299(5608): 900-902. [cited by applicant]
Qiu, et al. Construction of genetically engineered strains of Enterobacter cloacae (nifL˜(-)A ˜(c)), Acta Phytophysiologica Sinica, Jan. 1, 1999, 25(3):269-273. [cited by applicant]
Rodríguez-Navarro, D. N., et al., “Attachment of bacteria to the roots of higher plants,” FEMS Microbio Lett 272, pp. 127-136, DOI: 10.1111/j.1574-6968.2007.00761.x (2007). [cited by applicant]
Sanahuja, et al., “Bacillus thuringiensis: a century of research, development and commercial applications”. Plant Biotechnol J. (Apr. 2011); 9(3): 283-300. Epub Feb. 2, 2011. [cited by applicant]
Schuler, et al., “Insect-resistant transgenic plants”. Trends Biotechnol. (Apr. 1, 1998); 16(4): 168-175. [cited by applicant]
Setten, et al., Engineering Pseudomonas protegens Pf-5 for Nitrogen Fixation and its application to improve plant growth under nitrogen-deficient conditions, PLOS One 2013; 8(5): 1-14. [cited by applicant]
Shamseldin, A., “The Role of Different Genes Involved in Symbiotic Nitrogen Fixation—Review,” Global Journal of Biotechnology & Biochemistry, 8(4):84-94 (2013). [cited by applicant]
Stemmer, W.P., “DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution.” Proceedings of the National Academy of Sciences (Oct. 25, 1994); 91(22): 10747-10751. [cited by applicant]
Stemple, D. L., “Tilling—a high-throughput harvest for functional genomics,” Nature Reviews Genetics, 5, pp. 145-150, 7 pages (Feb. 2004). [cited by applicant]
Uhart, S. A., et al., “Nitrogen Deficiency in Maize: I. Effects on Crop Growth, Development, Dry Matter Partitioning, and Kernel Set,” Crop Sci., 35:1376-1383 (Sep. 1995). [cited by applicant]
Van Heeswijk et al. “Nitrogen Assimilation in [cited by applicant]
Watson, C. J., “Urease Activity and Inhibition—Principles and Practice,” Proceedings, No. 454, pp. 1-40, presented to the International Fertiliser Society, London, Nov. 28, 2000. [cited by applicant]
Yarza, Pablo, et al., “Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences.” Nature Reviews Microbiology (Sep. 2014); 12.9: 635-645. [cited by applicant]
Zehr lab NifH database, wwwzehr.pmc.ucsc.edu/nifH_Database_Public, Apr. 4, 2014, 8 pages. [cited by applicant]
Zhang, T., et al., “Involvement of the ammonium transporter AmtB in nitrogenase regulation and ammonium excretion in Pseudomonas stutzeri A1501,” Research in Microbiology 163, pp. 332-339, doi:10.1016/j.resmic.2012.05.0… [cited by applicant]
Extended European Search Report and Search Opinion, dated Jul. 30, 2024, for European Patent Application No. 21854590.3, 7 pages. [cited by applicant]
Office Action and Search Report, dated Dec. 1, 2023, for Chinese Patent Application No. 202080027155.8, 8 pages. [cited by applicant]
Office Action, dated Aug. 26, 2024, for Korean Patent Application No. 10-2021-7003763, 8 pages. [cited by applicant]
Office Action, dated Oct. 2, 2024, for Japanese Patent Application No. 2021-545687, 5 pages. [cited by applicant]
Office Action for Australian Patent Application No. 2019302747 dated Nov. 29, 2024, 4 pages. [cited by applicant]
Office Action for Canadian Patent Application No. 3128253 dated Sep. 5, 2024, 3 pages. [cited by applicant]
Office Action for Chinese Patent Application No. 201980053956.9 dated Sep. 9, 2023, 18 pages. [cited by applicant]
Office Action for Chinese Patent Application No. 202080027155.8 dated Sep. 25, 2024, 13 pages. [cited by applicant]
Office Action for Chinese Patent Application No. 202080027155.8 dated Feb. 28, 2024, 8 pages. [cited by applicant]
Office Action for European Patent Application No. 19833252.0 dated Oct. 23, 2024, 5 pages. [cited by applicant]
Office Action for Japanese Patent Application No. 2023-189380 dated Oct. 9, 2024, 4 pages. [cited by applicant]
Clancy et al., “The domains carrying the opposing activities in adenylyl transferase are separated by a central regulatory domain,” FEBS Journal, 2007, 274:2865-2877. [cited by applicant]
Communication pursuant to Article 94(3) EPC, mailed Feb. 16, 2023, for European Patent Application No. 19833252.0 (6 total pages). [cited by applicant]
Examination Report, dated Jul. 31, 2023, for Canadian Patent Application No. 3,105,529 (5 pages). [cited by applicant]
Cited By (3)
US 12,610,956 US 12,692,203 US 12,735,713