Recombinant nitrogen-fixing bacterial strain, inoculum containing the same and application methods
Recombinant bacterial strains comprising heterologous nif genes in its genome, and capable of fixing nitrogen. The strain may be, for example, a recombinant Pseudomonas fluorescens strain comprising heterologous nif genes in its genome. An inoculum and a method for increasing plant productivity are further described.
1. A method for increasing nitrogen fixation in plants, comprising applying to soil 1.6×10 8 -4×10 8 per liter of soil of a recombinant strain of Pseudomonas fluorescens that is capable of fixing nitrogen, said recombinant strain comprising the genomic segments PST_1302 to PST_1306 (SEQ ID NO: 5) and PST_1313 to PST_1359 (SEQ ID NO: 6) obtained from Pseudomonas stutzeri strain A1501.
2. The method according to claim 1 , wherein the plant is a monocotyledon.
3. The method according to claim 1 , wherein the plant is a dicotyledon.
4. The method according to claim 3 , wherein the plant is selected from the group consisting of Arabidopsis and Alfalfa.
5. The method according to claim 2 , wherein the plant is Fescue.
6. The method according to claim 1 , wherein said genomic segments consist of PST_1302 to PST_1306 (SEQ ID NO: 5) and PST_1313 to PST_1359 (SEQ ID NO: 6).