IP Library › Granted Patent US 12,331,230
Granted Patent B2
US 12,331,230 · App. 17/270,095 · Granted Jun 17, 2025

Protein-based adhesive and its modification

Inventors: Julie C. Liu (West Lafayette, IN); Sydney E. Hollingshead (Cleveland, OH); Charng-Yu Lin (West Lafayette, IN); Jonathan J. Wilker (West Lafayette, IN)
C09J189/00A61L24/108C07K14/78C09J11/04
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Quick Facts
Patent No.
US 12,331,230
App. No.
17/270,095
Granted
Jun 17, 2025
Kind
B2
Abstract

This invention relates to protein-based adhesives that are capable to adhere to a substrate in a dry, wet, moist, or an aqueous environment. Particularly, said adhesives comprise a crosslinking agent and an elastin-like polypeptide (ELP) that contain tyrosine, lysine, cysteine, dihydroxyphenylalanine (DOPA) or trihydroxyphenylalanine (TOPA) amino acid residue, or a combination thereof, on the polypeptide chain of said ELP. Among others, the adhesives disclosed herein may find broad applications in medical treatments and surgical operations. Compositions and methods of use are within the scope of this application.

Claims (7)

1. An adhesive comprising (i) a crosslinking agent selected from tetrakis(hydroxymethyl)phosphonium chloride (THPC), an N-hydroxysuccinyl ester (NHS), genipin, pyridyl disulfide, a maleimide, a vinylsulfone, a haloacetyl, and a combination thereof, (ii) an elastin-like polypeptide (ELP), wherein polypeptide chain of said ELP comprises (a) a tyrosine residue, a derivative thereof, or a combination thereof and (b) at least one of a lysine residue, a cysteine residue, or a combination thereof, and (iii) an oxidizing agent selected from a ferric salt, a hydrogen peroxide, and a sodium periodate, wherein the ELP comprises at least one of SEQ ID NOs: 4, 6, 8 or 10.

2. The adhesive of claim 1 , wherein the derivative of the tyrosine residue is dihydroxyphenylalanine (DOPA), trihydroxyphenylalanine (TOPA), or a combination thereof.

3. A method of generating an adhesive comprising the steps of:

providing a crosslinking agent selected from tetrakis(hydroxymethyl)phosphonium chloride (THPC), an N-hydroxysuccinyl ester (NHS), genipin, pyridyl disulfide, a maleimide, a vinylsulfone, a haloacetyl, and a combination thereof;

adding an elastin-like polypeptide (ELP), wherein the polypeptide chain of said ELP comprises (a) a tyrosine residue, a derivative thereof, or a combination thereof and (b) at least one of a lysine residue, a cysteine residue, or a combination thereof; and

adding an oxidizing agent selected from a ferric salt, a hydrogen peroxide, and a sodium periodate, wherein the ELP comprises at least one of SEQ ID NOs: 4, 6, 8 or 10.

4. The method according to claim 3 , wherein the derivative of the tyrosine residue is dihydroxyphenylalanine (DOPA), trihydroxyphenylalanine (TOPA), or a combination thereof.

Assignments (2)
CONFIRMATORY LICENSE Recorded Feb 23, 2022
From: PURDUE UNIVERSITY
To: NATIONAL SCIENCE FOUNDATION
Reel/Frame 059315/0585 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 1, 2021
From: WILKER, JONATHAN JAMES; LIU, JULIE C; LIN, CHARNG-YU; HOLLINGSHEAD, SYDNEY E.
To: PURDUE RESEARCH FOUNDATION
Reel/Frame 057664/0819 →
Continuity (2)
Provisional Application 62721112 · Aug 22, 2018
Related Publication 20210207013A1 · Jul 8, 2021
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