Compositions and methods for controlling plant pests and improving plant health
Compositions and methods for controlling plant pests and/or improving at least one agronomic trait of interest in a plant are provided. Such compositions and methods comprise a bacterial strain that can be used as an inoculant for plants. Therefore, methods for growing a plant susceptible to a plant pest and/or plant disease caused by a plant pest and methods for controlling plant pests and/or plant disease on a plant susceptible to the plant pest and/or plant disease are also provided.
1. A method for controlling a plant pest population comprising contacting said population with a composition comprising an effective amount of a biocontrol agent, wherein the biocontrol agent comprises a Pseudomonas chlororaphis bacterial strain AIP059286 (Northern Region Research Laboratory (NRRL) No. B-67653), wherein said composition controls said plant pest.
2. The method of claim 1 , wherein the bacterial strain is present in the composition at about 10 5 colony forming units per gram (CFU/gram) to about 10 12 CFU/gram or in about 10 5 CFU/ml to about 10 12 CFU/ml.
3. The method of claim 2 , wherein said effective amount of the bacterial strain comprises at least about 10 4 to 10 16 CFU per hectare.
4. The method of claim 1 , wherein the composition further comprises a pesticide, a fungicide, an insecticide, or a herbicide.
5. The method of claim 4 , wherein the fungicide comprises prothioconazole, azoxystrobin, fluopicolide, chlorothalonil, fosetyl, fenhexamid, flutriafol, difenoconazole, tebuconazole, tetraconazole, pyraclostrobin, trifloxystrobin, propiconazole, fluoxastrobin, flutolanil, metconazole, or metrafenone.
6. A method for growing a plant susceptible to a plant pest comprising applying to a plant, a plant part, a seed, or an area of cultivation a biocontrol agent, wherein the biocontrol agent comprises:
(a) an effective amount of a Pseudomonas chlororaphis bacterial strain AIP059286 (NRRL No. B-67653); or
(b) an effective amount of a supernatant, fermentation product, filtrate, or extract derived from a whole cell culture of a bacterial strain deposited as NRRL No. B-67653;
wherein said effective amount controls the plant pest.
7. The method of claim 6 , wherein said effective amount of the bacterial strain comprises at least about 10 4 to 10 16 CFU per hectare.
8. A method of controlling a plant pest comprising applying to a plant, a plant part, a seed, or an area of cultivation a biocontrol agent, wherein the biocontrol agent comprises:
(a) an effective amount of a Pseudomonas chlororaphis bacterial strain AIP059286 (NRRL No. B-67653); or
(b) an effective amount of a supernatant, fermentation product, filtrate, or extract derived from a whole cell culture of a bacterial strain deposited as NRRL No. B-67653;
wherein said effective amount controls the plant pest.
9. The method of claim 8 , wherein said effective amount of the bacterial strain comprises at least about 10 4 to 10 16 CFU per hectare.
10. The method of claim 8 , wherein the biocontrol agent is applied to the plant or plant part after harvest.
11. A method of treating or preventing a plant disease comprising applying to a plant or plant part having a plant pest or plant disease or at risk of developing a plant pest or plant disease:
(a) an effective amount of a biocontrol agent comprising a Pseudomonas chlororaphis bacterial strain AIP059286 (NRRL No. B-67653); or
(b) an effective amount of a supernatant, fermentation product, filtrate, or extract derived from a whole cell culture of a bacterial strain deposited as NRRL No. B-67653;
wherein the effective amount controls the plant pest.
12. A stable spray-dried formulation comprising a biocontrol agent, wherein the biocontrol agent comprises
(a) a Pseudomonas chlororaphis bacterial strain AIP059286 (NRRL No. B-67653); or
(b) a supernatant, fermentation product, filtrate, or extract derived from a whole cell culture of a bacterial strain deposited as NRRL No. B-67653.
13. The stable spray-dried formulation of claim 12 , wherein said spray-dried formulation is dried to a water activity of 0.3 or less.
14. The stable spray-dried formulation of claim 12 , wherein the formulation is a wettable powder or a granule.
15. The stable spray-dried formulation of claim 12 , wherein the biocontrol agent is present at about 10 5 CFU/gram to about 10 12 CFU/gram or at about 10 5 CFU/ml to about 10 12 CFU/ml.
16. The stable spray-dried formulation of claim 12 , wherein the formulation further comprises a pesticide, a fungicide, an insecticide or an herbicide.
17. The stable spray-dried formulation of claim 16 , wherein the fungicide comprises prothioconazole, azoxystrobin, fluopicolide, chlorothalonil, fosetyl, fenhexamid, flutriafol, difenoconazole, tebuconazole, tetraconazole, pyraclostrobin, trifloxystrobin, propiconazole, fluoxastrobin, flutolanil, metconazole, or metrafenone.
18. A coated seed comprising a seed and a coating on the seed, wherein the coating comprises a spray-dried formulation comprising a biocontrol agent, wherein the biocontrol agent comprises a Pseudomonas chlororaphis bacterial strain AIP059286 (NRRL No. B-67653).
19. The coated seed of claim 18 , wherein the coating further comprises a pesticide, a fungicide, an insecticide or an herbicide.
20. The coated seed of claim 19 , wherein the fungicide comprises prothioconazole, azoxystrobin, fluopicolide, chlorothalonil, fosetyl, fenhexamid, flutriafol, difenoconazole, tebuconazole, tetraconazole, pyraclostrobin, trifloxystrobin, propiconazole, fluoxastrobin, flutolanil, metconazole, or metrafenone.