Guard column and method for producing guard column
Provided is a guard column including a filling part having a length of 2.0 cm to 3.5 cm formed of a filler, in which the filler is made of porous silica gel having a hydrophilized surface and an average particle size of 1.5 μm to 2.5 μm, and a pressure difference when an aqueous solution is fed at a linear flow rate of 2.1 cm/min is 4.0 MPa or more.
1. A guard column, comprising
a filling part having a length of 2.0 cm to 3.5 cm formed of a filler, wherein
the filler includes a porous silica gel,
the porous silica gel has a hydrophilized surface, an average particle size of 1.5 μm to 2.5 μm, an average pore size of 25 nm to 35 nm, and a specific surface area of 100 m 2 /g to 300 m 2 /g, and
a pressure difference when an aqueous solution is fed to the guard column at a linear flow rate of 2.1 cm/min is 4.0 MPa or more.
2. A guard column, comprising a filling part having a length of 2.0 cm to 3.5 cm formed of a filler, wherein
the filler includes a porous silica gel having a hydrophilized surface and an average particle size of 1.5 μm to 2.5 μm,
a pressure difference when an aqueous solution is fed at a linear flow rate of 2.1 cm/min is 4.0 MPa or more, and
the guard column is used for size-exclusion chromatography in which a column using a filler containing porous silica gel and a light scattering detector are used with the guard column.
3. A liquid chromatograph comprising the guard column according to claim 1 , a size-exclusion chromatography column filled with a filler containing porous silica gel, and a light scattering detector in this order from an upstream side.
4. A method of analyzing a polymer compound, which comprises:
analyzing the polymer compound with size-exclusion chromatography using the liquid chromatograph according to claim 3 .
5. A method for producing a guard column according to claim 1 , comprising:
a step of filling the filler including the porous silica gel having a hydrophilized surface and the average particle size of 1.5 μm to 2.5 μm at a filling pressure of 12 MPa to 40 MPa per 1 cm in length to form the filling part having a length of 2.0 cm to 3.5 cm.
6. The guard column according to claim 1 , wherein a diameter of the filling part is 0.4 cm to 0.9 cm.
7. A guard column, comprising a filling part having a length of 2.0 cm to 3.5 cm formed of a filler, wherein
the filler includes a porous silica gel having a hydrophilized surface and an average particle size of 1.5 μm to 2.5 μm,
a pressure difference when an aqueous solution is fed at a linear flow rate of 2.1 cm/min is 4.0 MPa or more, and
wherein the porous silica gel having a hydrophilized surface is a silica gel which is obtained by treating porous silica gel, whose surface is not hydrophilized, with a silane coupling agent to form a functional group derived from the silane coupling agent and further reacting the functional group with a hydrophilic compound.
8. The guard column according to claim 7 , wherein
the silane coupling agent has an epoxy group, and
the hydrophilic compound is water, a polyhydric alcohol compound, or a compound having an epoxy group.
9. The guard column according to claim 1 , wherein the porous silica gel having a hydrophilized surface is a silica gel which is obtained by surface-treating porous silica gel, whose surface is not hydrophilized, with glycidoxypropyltrimethoxysilane and ring-opening an epoxy group formed on a surface thereof with water.
10. The method of analyzing a polymer compound according to claim 4 , wherein the polymer compound is a protein.
11. The column according to claim 1 , wherein the guard column is connected to an analytical column.
12. The column according to claim 11 , wherein
the guard column is disposed on an upstream side of an analytical column,
the analytical column is filled with a filler containing a porous silica gel.
13. The column according to claim 11 , wherein
the pressure difference is a difference between a pressure A and a pressure B, wherein
the pressure A is a pressure when an aqueous solution is fed at a linear flow rate of 2.1 cm/min into the guard column which is connected with an analyst column, and
the pressure B is a pressure when the aqueous solution is fed at the linear flow rate of 2.1 cm/min into an empty column which is connected with the analyst column, wherein the empty column is the guard column from which the filler has been removed.
14. A size-exclusion chromatography column comprising the guard column according to claim 1 , and an analytical column.