Granted Patent B2

US 12,350,664 · App. 17/331,554 · Granted Jul 8, 2025

Inventors: Brian Alexander Pesch (San Francisco, CA); Fabian Gerlinghaus (South San Francisco, CA); Nicholas Scott Reimnitz (South San Francisco, CA)

Assignee: Cellares Corporation

B01L3/502715A61L2/04A61L2/20A61L2/206A61L2/208B01J19/004B01L13/00C12M23/44C12M23/50C12M41/48C12N5/00C12N5/0081A61L2/08A61L2202/11A61L2202/122A61L2202/123A61L2202/14B01J2219/0002B01J2219/0074B01L2200/027B01L2300/049

Abstract

Claims (33)

1. A cartridge for use in cell processing, comprising:

a plurality of cell processing modules defining an enclosed housing, wherein at least one module of the plurality of cell processing modules is selected from the group consisting of a cell selection module, an electroporation module, and a counterflow centrifugal elutriation (CCE) module, and at least one module of the plurality of cell processing modules comprises a bioreactor module;

a liquid transfer bus comprising a manifold, at least one fluid conduit, and at least one valve; and

a pump system comprising an opening in the enclosed housing and tubing in fluidic communication with the liquid transfer bus, wherein at least a portion of the tubing is externally exposed from the opening of the housing so as to be engageable by a pump actuator of an instrument within a cell processing workcell to move fluid within the cartridge,

wherein each cell processing module is fluidically coupled to the manifold of the liquid transfer bus, and

wherein the cartridge is sized and shaped to pass through a feedthrough of the cell processing workcell.

2. The cartridge of claim 1 , wherein the cartridge comprises one or more sterile liquid transfer ports.

3. The cartridge of claim 1 , wherein the plurality of cell processing modules of the cartridge comprises the cell selection module.

4. The cartridge of claim 3 , wherein the cell selection module is a magnetic-activated cell selection module.

5. The cartridge of claim 1 , wherein the plurality of cell processing modules of the cartridge further comprises a cell sorting module.

6. The cartridge of claim 5 , wherein the cell sorting module is a fluorescence activated cell sorting module.

7. The cartridge of claim 1 , wherein the plurality of cell processing modules of the cartridge comprises the electroporation module.

8. The cartridge of claim 1 , wherein the plurality of cell processing modules of the cartridge comprises the CCE module.

9. The cartridge of claim 1 , wherein the cartridge comprises a second bioreactor module.

10. The cartridge of claim 1 , wherein the plurality of cell processing modules of the cartridge comprises the bioreactor module, the cell selection module, and one or more of the CCE module and the electroporation module.

11. The cartridge of claim 1 , wherein one or more of the plurality of cell processing modules are moveable within the cartridge.

12. The cartridge of claim 1 , wherein actuation of the actuator of the instrument causes the at least one valve to move between a first position and a second position.

13. The cartridge of claim 8 , wherein the CCE module is configured to separate cells based on one or more of cell size and density.

14. The cartridge of claim 8 , wherein the CCE module comprises a subhousing comprising a rotor configured to separate cells from a fluid.

15. The cartridge of claim 14 , wherein the subhousing comprises one or more apertures configured to facilitate visualization of the rotor.

16. The cartridge of claim 14 , wherein one or more portions of the rotor is optically transparent.

17. The cartridge of claim 14 , wherein the rotor comprises a first side comprising a first fluid port and a second side comprising a second fluid port, the second side opposite the first side.

18. The cartridge of claim 17 , wherein at least a portion of the first fluid port and the second fluid port extend coaxially in parallel with each other.

19. The cartridge of claim 14 , wherein the rotor is configured to magnetically-engage a magnet of a corresponding instrument of the workcell.

20. The cartridge of claim 14 , wherein the rotor comprises an asymmetric shape.

21. The cartridge of claim 4 , wherein the magnetic-activated cell selection module comprises a flow cell.

22. The cartridge of claim 21 , wherein the flow cell comprises at least one elongate cavity.

23. The cartridge of claim 22 , wherein the at least one elongate cavity of the flow cell comprises a set of linear channels.

24. The cartridge of claim 21 , wherein the flow cell comprises a plurality of inlets and a plurality of outlets.

25. The cartridge of claim 1 , wherein the cartridge is movable relative to the instrument within the workcell by a robot.

26. The cartridge of claim 22 , wherein the elongate cavity is substantially empty prior to flow of biological material therethrough.

27. The cartridge of claim 1 , wherein the manifold is disposed within an exterior wall of the housing of the cartridge.

28. The cartridge of claim 1 , wherein the tubing is arranged around a circumference of the opening of the housing.

Assignments (2)

Continuity (4)

References Cited (287)

Cited By (5)

US 1,109,621 US 12,497,587 US 12,551,887 US 12,559,710 US 12,570,948