Recombinant fusion proteins for producing milk proteins in plants
Provided herein are compositions and methods for producing milk proteins in plants, which allow for safe, sustainable and humane production of milk proteins for commercial use, such as use in food compositions. The disclosure provides recombinant fusion proteins comprising a milk protein, or fragment thereof and a structured mammalian, avian, plant, or fungal protein, or fragment thereof. The disclosure also provides methods for producing the recombinant fusions proteins, and food compositions comprising the same.
1. A food composition, comprising: a fusion protein, said fusion protein comprising i) a bovine casein component and ii) a bovine β-lactoglobulin component.
2. The food composition of claim 1 , wherein the food composition is selected from the group consisting of: cheese, processed cheese product, fermented dairy product, directly acidified counterpart of fermented dairy product, cottage cheese, dressing, frozen dairy product, frozen dessert, dessert, baked good, topping, icing, filling, low-fat spread, dairy-based dry mix, soup, sauce, salad dressing, geriatric nutrition, cream, creamer, analog dairy product, follow-up formula, baby formula, infant formula, milk, dairy beverage, acid dairy drink, smoothie, milk tea, butter, margarine, butter alternative, growing up milk, low-lactose product, low-lactose beverage, medical and clinical nutrition product, protein bar, nutrition bar, sport beverage, confection, meat product, analog meat product, meal replacement beverage, weight management food and beverage, dairy product, cultured buttermilk, sour cream, yogurt, skyr, Leben, lassi, kefir, powder containing a milk protein, and low-lactose product.
3. The food composition of claim 1 , wherein the food composition is a solid.
4. The food composition of claim 1 , wherein the food composition is a liquid.
5. The food composition of claim 1 , wherein the food composition is a powder.
6. The food composition of claim 1 , wherein the food composition is a dairy product.
7. The food composition of claim 1 , wherein the food composition is an analog dairy product.
8. The food composition of claim 1 , wherein the food composition is a low lactose product.
9. The food composition of claim 1 , wherein the food composition is a milk.
10. The food composition of claim 1 , wherein the food composition is a cheese.
11. The food composition of claim 1 , wherein the food composition is fermented.
12. A food composition, comprising: a plant expressed fusion protein, said plant expressed fusion protein comprising i) a bovine casein component and ii) a bovine β-lactoglobulin component.
13. The food composition of claim 12 , wherein the food composition is selected from the group consisting of: cheese, processed cheese product, fermented dairy product, directly acidified counterpart of fermented dairy product, cottage cheese, dressing, frozen dairy product, frozen dessert, dessert, baked good, topping, icing filling low-fat spread, dairy-based dry mix, soup, sauce, salad dressing, geriatric nutrition, cream, creamer, analog dairy product, follow-up formula, baby formula, infant formula, milk, dairy beverage, acid dairy drink, smoothie, milk tea, butter, margarine, butter alternative, growing up milk, low-lactose product, low-lactose beverage, medical and clinical nutrition product, protein bar, nutrition bar, sport beverage, confection, meat product, analog meat product, meal replacement beverage, weight management food and beverage, dairy product, cultured buttermilk, sour cream, yogurt, skyr, leben, lassi, kefir, powder containing a milk protein, and low-lactose product.
14. The food composition of claim 12 , wherein the food composition is a solid.
15. The food composition of claim 12 , wherein the food composition is a liquid.
16. The food composition of claim 12 , wherein the food composition is a powder.
17. The food composition of claim 12 , wherein the food composition is a dairy product.
18. The food composition of claim 12 , wherein the food composition is an analog dairy product.
19. The food composition of claim 12 , wherein the food composition is a low lactose product.
20. The food composition of claim 12 , wherein the food composition is a milk.
21. The food composition of claim 12 , wherein the food composition is a cheese.
22. The food composition of claim 12 , wherein the food composition is fermented.
23. The food composition of claim 12 , wherein the plant expressed fusion protein is a soybean expressed fusion protein.