IP Library › Granted Patent US 12,227,750
Granted Patent B2
US 12,227,750 · App. 18/456,080 · Granted Feb 18, 2025

Recombinant micelle and method of in vivo assembly

Inventor: Cory J. Tobin (Pasadena, CA)
Assignee: Mozza Foods, Inc.
C12N15/8202B01D61/145B01D61/147B01D61/149C12N15/8218B01D2315/16
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Quick Facts
Patent No.
US 12,227,750
App. No.
18/456,080
Granted
Feb 18, 2025
Kind
B2
Abstract

A method of in vivo assembly of a recombinant micelle including: introducing a plasmid into a plant cell, wherein: the plasmid includes a segment of deoxyribonucleic acid (DNA) for encoding a ribonucleic acid (RNA) for a protein in a casein micelle, the segment of DNA is transcribed and translated; forming recombinant casein proteins in the plant cell, wherein: the recombinant casein proteins include a κ-casein and at least one of an αS 1 -casein, an αS 2 -casein, a β-casein; and assembling in vivo a recombinant micelle within the plant cell, wherein: an outer layer of the recombinant micelle is enriched with the κ-casein, an inner matrix of the recombinant micelle include at least one of the αS 1 -casein, the αS 2 -casein, the β-casein.

Claims (11)

1. A method of expressing casein protein in a soybean plant, comprising:

a) expressing casein protein in a plant cell;

b) accumulating casein protein in a protein storage vacuole; and

c) suppressing expression of a native plant protein or a native plant peptide in the plant cell, wherein the suppressing expression of the native plant protein or native plant peptide in the plant cell increases expression of casein protein in the plant cell.

2. The method in claim 1 , wherein the casein protein is a bovine k-casein.

3. The method in claim 1 , wherein the casein protein is a bovine αS1-casein.

4. The method in claim 1 , wherein the casein protein is a bovine αS2-casein.

5. The method in claim 1 , wherein the casein protein is a bovine β-casein.

6. The method in claim 1 , wherein suppressing expression of a native plant or native plant peptide comprises using RNAi to target messenger RNA of the native plant protein or the native plant peptide.

7. The method in claim 1 , wherein the native plant protein or the native plant peptide is β-conglycinin.

8. The method in claim 1 , wherein the native plant protein or the native plant peptide is glycinin.

Continuity (5)
Continuation 18075250 · Dec 5, 2022
Continuation 17717000 · Apr 8, 2022
Continuation 16741680 · Jan 13, 2020
Provisional Application 62939247 · Nov 22, 2019
Related Publication 20230407319A1 · Dec 21, 2023
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